Systematic Analysis of Metabolic Bottlenecks in the Methylerythritol 4-Phosphate (MEP) Pathway of Zymomonas mobilis

Khana, Daven B.; Tatli, Mehmet; Rivera Vazquez, Julio; Weraduwage, Sarathi M.; Stern, Noah; Hebert, Alexander S.; Angelica Trujillo, Edna; Stevenson, David M.; Coon, Joshua J.; Sharky, Thomas D.; Amador-Noguez, Daniel; Porto, ed., Carla

doi:10.1128/msystems.00092-23

Systematic Analysis of Metabolic Bottlenecks in the Methylerythritol 4-Phosphate (MEP) Pathway of Zymomonas mobilis

Journal Article · Thu Apr 27 00:00:00 EDT 2023 · mSystems

DOI:https://doi.org/10.1128/msystems.00092-23· OSTI ID:1964201

Khana, Daven B. ^[1]; Tatli, Mehmet ^[2]; Rivera Vazquez, Julio ^[1]; Weraduwage, Sarathi M. ^[3]; Stern, Noah ^[2]; Hebert, Alexander S. ^[4]; Angelica Trujillo, Edna ^[5]; Stevenson, David M. ^[2]; Coon, Joshua J. ^[6]; Sharky, Thomas D. ^[3]; ^[2]; Porto, ed., Carla

DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA, Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA, Microbiology Doctoral Training Program, University of Wisconsin-Madison, Wisconsin, USA
DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA, Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA
MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan, USA
DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA, Genome Center of Wisconsin, Madison, Wisconsin, USA
DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA, Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA
DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA, Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA, Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA, Morgridge Institute for Research, Madison, Wisconsin, USA

Engineered microorganisms have the potential to convert renewable substrates into biofuels and valuable bioproducts, which offers an environmentally sustainable alternative to fossil-fuel-derived products. Isoprenoids are a diverse class of biologically derived compounds that have commercial applications as various commodity chemicals, including biofuels and biofuel precursor molecules.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0018998; SC0018409

OSTI ID:: 1964201

Alternate ID(s):: OSTI ID: 2420812

Journal Information:: mSystems, Journal Name: mSystems Journal Issue: 2 Vol. 8; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (81)

Bioorganometallic Chemistry with IspG and IspH: Structure, Function, and Inhibition of the [Fe ₄ S ₄ ] Proteins Involved in Isoprenoid Biosynthesis Wang, Weixue; Oldfield, Eric Angewandte Chemie International Edition, Vol. 53, Issue 17 https://doi.org/10.1002/anie.201306712	journal	January 2014
Improved NADPH supply for xylitol production by engineered Escherichia coli with glycolytic mutations Chin, Jonathan W.; Cirino, Patrick C. Biotechnology Progress, Vol. 27, Issue 2, p. 333-341 https://doi.org/10.1002/btpr.559	journal	February 2011
Zymomonas mobilis: an alternative ethanol producer Panesar, Parmjit S.; Marwaha, Satwinder S.; Kennedy, John F. Journal of Chemical Technology & Biotechnology, Vol. 81, Issue 4 https://doi.org/10.1002/jctb.1448	journal	January 2006
El-MAVEN: A Fast, Robust, and User-Friendly Mass Spectrometry Data Processing Engine for Metabolomics Agrawal, Shubhra; Kumar, Sahil; Sehgal, Raghav High-Throughput Metabolomics https://doi.org/10.1007/978-1-4939-9236-2_19	book	January 2019
Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS) Jannasch, Amber; Sedlak, Miroslav; Adamec, Jiri Methods in Molecular Biology https://doi.org/10.1007/978-1-61737-985-7_9	book	December 2010
High productivity ethanol fermentations with Zymomonas mobilis using continuous cell recycle Lee, K. J.; Lefebvre, M.; Tribe, D. E. Biotechnology Letters, Vol. 2, Issue 11 https://doi.org/10.1007/BF00129544	journal	November 1980
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis Julsing, Mattijs K.; Rijpkema, Michael; Woerdenbag, Herman J. Applied Microbiology and Biotechnology, Vol. 75, Issue 6 https://doi.org/10.1007/s00253-007-0953-5	journal	April 2007
Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway Zhao, Yaru; Yang, Jianming; Qin, Bo Applied Microbiology and Biotechnology, Vol. 90, Issue 6 https://doi.org/10.1007/s00253-011-3199-1	journal	April 2011
Significantly enhanced production of isoprene by ordered coexpression of genes dxs, dxr, and idi in Escherichia coli Lv, Xiaomei; Xu, Haoming; Yu, Hongwei Applied Microbiology and Biotechnology, Vol. 97, Issue 6 https://doi.org/10.1007/s00253-012-4485-2	journal	November 2012
Enhanced C30 carotenoid production in Bacillus subtilis by systematic overexpression of MEP pathway genes Xue, Dan; Abdallah, Ingy I.; de Haan, Ilse E. M. Applied Microbiology and Biotechnology, Vol. 99, Issue 14 https://doi.org/10.1007/s00253-015-6531-3	journal	April 2015
Metabolic engineering for the production of isoprene and isopentenol by Escherichia coli Li, Meijie; Nian, Rui; Xian, Mo Applied Microbiology and Biotechnology, Vol. 102, Issue 18 https://doi.org/10.1007/s00253-018-9200-5	journal	July 2018
The Ribosome Binding Site Calculator Salis, Howard M. Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00002-4	book	May 2011
Measurement of isoprenoid content in leaves of Mediterranean Quercus spp. by a novel and sensitive method and estimation of the isoprenoid partition between liquid and gas phase inside the leaves Loreto, Francesco; Ciccioli, Paolo; Brancaleoni, Enzo Plant Science, Vol. 136, Issue 1 https://doi.org/10.1016/S0168-9452(98)00092-2	journal	August 1998
Measuring dimethylallyl diphosphate available for isoprene synthesis Weise, Sean E.; Li, Ziru; Sutter, Allison E. Analytical Biochemistry, Vol. 435, Issue 1 https://doi.org/10.1016/j.ab.2012.11.031	journal	April 2013
Occurrence, biosynthesis and function of isoprenoid quinones Nowicka, Beatrycze; Kruk, Jerzy Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1797, Issue 9 https://doi.org/10.1016/j.bbabio.2010.06.007	journal	September 2010
Cloning and functional characterization of an enzyme from Helicobacter pylori that catalyzes two steps of the methylerythritol phosphate pathway for isoprenoid biosynthesis Pérez-Gil, Jordi; Bergua, María; Boronat, Albert Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1800, Issue 9 https://doi.org/10.1016/j.bbagen.2010.06.008	journal	September 2010
Cloning and expression of IspDF from Mesorhizobium loti. Characterization of a bifunctional protein that catalyzes non-consecutive steps in the methylerythritol phosphate pathway Testa, Charles A.; Lherbet, Christian; Pojer, Florence Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1764, Issue 1 https://doi.org/10.1016/j.bbapap.2005.08.006	journal	January 2006
Recent applications of metabolomics to advance microbial biofuel production Martien, Julia I.; Amador-Noguez, Daniel Current Opinion in Biotechnology, Vol. 43 https://doi.org/10.1016/j.copbio.2016.11.006	journal	February 2017
fldA is an essential gene required in the 2- C -methyl- D -erythritol 4-phosphate pathway for isoprenoid biosynthesis Puan, Kia-Joo; Wang, Hong; Dairi, Tohru FEBS Letters, Vol. 579, Issue 17 https://doi.org/10.1016/j.febslet.2005.05.047	journal	June 2005
Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: Direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG Seemann, Myriam; Tse Sum Bui, Bernadette; Wolff, Murielle FEBS Letters, Vol. 580, Issue 6 https://doi.org/10.1016/j.febslet.2006.01.082	journal	February 2006
Carotenoid accumulation in bacteria with enhanced supply of isoprenoid precursors by upregulation of exogenous or endogenous pathways Rodríguez-Villalón, Antía; Pérez-Gil, Jordi; Rodríguez-Concepción, Manuel Journal of Biotechnology, Vol. 135, Issue 1 https://doi.org/10.1016/j.jbiotec.2008.02.023	journal	May 2008
Enhanced performance of the methylerythritol phosphate pathway by manipulation of redox reactions relevant to IspC, IspG, and IspH Zhou, Jia; Yang, Liyang; Wang, Chonglong Journal of Biotechnology, Vol. 248 https://doi.org/10.1016/j.jbiotec.2017.03.005	journal	April 2017
Identification and elimination of metabolic bottlenecks in the quinone modification pathway for enhanced coenzyme Q10 production in Rhodobacter sphaeroides Lu, Wenqiang; Ye, Lidan; Lv, Xiaomei Metabolic Engineering, Vol. 29 https://doi.org/10.1016/j.ymben.2015.03.012	journal	May 2015
Metabolic engineering of Escherichia coli for high-level astaxanthin production with high productivity Park, Seon Young; Binkley, Robert M.; Kim, Won Jun Metabolic Engineering, Vol. 49 https://doi.org/10.1016/j.ymben.2018.08.002	journal	September 2018
OptSSeq explores enzyme expression and function landscapes to maximize isobutanol production rate Ghosh, Indro N.; Martien, Julia; Hebert, Alexander S. Metabolic Engineering, Vol. 52 https://doi.org/10.1016/j.ymben.2018.12.008	journal	March 2019
2H and 13C metabolic flux analysis elucidates in vivo thermodynamics of the ED pathway in Zymomonas mobilis Jacobson, Tyler B.; Adamczyk, Paul A.; Stevenson, David M. Metabolic Engineering, Vol. 54 https://doi.org/10.1016/j.ymben.2019.05.006	journal	July 2019
Regulated redirection of central carbon flux enhances anaerobic production of bioproducts in Zymomonas mobilis Liu, Yang; Ghosh, Indro Neil; Martien, Julia Metabolic Engineering, Vol. 61 https://doi.org/10.1016/j.ymben.2020.06.005	journal	September 2020
Global metabolite analysis: the influence of extraction methodology on metabolome profiles of Escherichia coli Prasad Maharjan, Ram; Ferenci, Thomas Analytical Biochemistry, Vol. 313, Issue 1 https://doi.org/10.1016/s0003-2697(02)00536-5	journal	February 2003
Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the ( E )-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein Wolff, Murielle; Seemann, Myriam; Tse Sum Bui, Bernadette FEBS Letters, Vol. 541, Issue 1-3 https://doi.org/10.1016/s0014-5793(03)00317-x	journal	April 2003
Isoprene measurement by ozone-induced chemiluminescence Hills, Alan J.; Zimmerman, Patrick R. Analytical Chemistry, Vol. 62, Issue 10 https://doi.org/10.1021/ac00209a017	journal	May 1990
Acidic Acetonitrile for Cellular Metabolome Extraction from Escherichia coli Rabinowitz, Joshua D.; Kimball, Elizabeth Analytical Chemistry, Vol. 79, Issue 16 https://doi.org/10.1021/ac070470c	journal	August 2007
Rapid Targeted Quantitation of Protein Overexpression with Direct Infusion Shotgun Proteome Analysis (DISPA-PRM) Trujillo, Edna A.; Hebert, Alexander S.; Rivera Vazquez, Julio C. Analytical Chemistry, Vol. 94, Issue 4 https://doi.org/10.1021/acs.analchem.1c03243	journal	January 2022
Overexpression of the Key Enzymes in the Methylerythritol 4-phosphate Pathway in Corynebacterium glutamicum for Improving Farnesyl Diphosphate-Derived Terpene Production Lim, Hyeonbae; Park, Jaehyun; Woo, Han Min Journal of Agricultural and Food Chemistry, Vol. 68, Issue 39 https://doi.org/10.1021/acs.jafc.0c04307	journal	August 2020
OptSSeq: High-Throughput Sequencing Readout of Growth Enrichment Defines Optimal Gene Expression Elements for Homoethanologenesis Ghosh, Indro Neil; Landick, Robert ACS Synthetic Biology, Vol. 5, Issue 12 https://doi.org/10.1021/acssynbio.6b00121	journal	July 2016
Metabolic Engineering of the MEP Pathway in Bacillus subtilis for Increased Biosynthesis of Menaquinone-7 Ma, Yanwei; McClure, Dale D.; Somerville, Mark V. ACS Synthetic Biology, Vol. 8, Issue 7 https://doi.org/10.1021/acssynbio.9b00077	journal	June 2019
2 C -Methyl- d -erythritol 4-Phosphate Enhances and Sustains Cyclodiphosphate Synthase IspF Activity Bitok, J. Kipchirchir; Meyers, Caren Freel ACS Chemical Biology, Vol. 7, Issue 10 https://doi.org/10.1021/cb300243w	journal	August 2012
IspH Protein of Escherichia c oli : Studies on Iron−Sulfur Cluster Implementation and Catalysis Gräwert, Tobias; Kaiser, Johannes; Zepeck, Ferdinand Journal of the American Chemical Society, Vol. 126, Issue 40 https://doi.org/10.1021/ja0471727	journal	October 2004
Eddy covariance measurement of isoprene fluxes Guenther, Alex B.; Hills, Alan J. Journal of Geophysical Research: Atmospheres, Vol. 103, Issue D11 https://doi.org/10.1029/97JD03283	journal	June 1998
Microbial engineering for the production of advanced biofuels Peralta-Yahya, Pamela P.; Zhang, Fuzhong; del Cardayre, Stephen B. Nature, Vol. 488, Issue 7411 https://doi.org/10.1038/nature11478	journal	August 2012
Rewriting yeast central carbon metabolism for industrial isoprenoid production Meadows, Adam L.; Hawkins, Kristy M.; Tsegaye, Yoseph Nature, Vol. 537, Issue 7622 https://doi.org/10.1038/nature19769	journal	September 2016
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification Cox, Jürgen; Mann, Matthias Nature Biotechnology, Vol. 26, Issue 12 https://doi.org/10.1038/nbt.1511	journal	November 2008
A unified mechanism of action for volatile isoprenoids in plant abiotic stress Vickers, Claudia E.; Gershenzon, Jonathan; Lerdau, Manuel T. Nature Chemical Biology, Vol. 5, Issue 5 https://doi.org/10.1038/nchembio.158	journal	April 2009
Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli Bennett, Bryson D.; Kimball, Elizabeth H.; Gao, Melissa Nature Chemical Biology, Vol. 5, Issue 8 https://doi.org/10.1038/nchembio.186	journal	June 2009
Unusual transformations in the biosynthesis of the antibiotic phosphinothricin tripeptide Blodgett, Joshua A. V.; Thomas, Paul M.; Li, Gongyong Nature Chemical Biology, Vol. 3, Issue 8 https://doi.org/10.1038/nchembio.2007.9	journal	July 2007
Metabolite concentrations, fluxes and free energies imply efficient enzyme usage Park, Junyoung O.; Rubin, Sara A.; Xu, Yi-Fan Nature Chemical Biology, Vol. 12, Issue 7 https://doi.org/10.1038/nchembio.2077	journal	May 2016
Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan Nature Methods, Vol. 6, Issue 5, p. 343-345 https://doi.org/10.1038/nmeth.1318	journal	April 2009
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach Bennett, Bryson D.; Yuan, Jie; Kimball, Elizabeth H. Nature Protocols, Vol. 3, Issue 8 https://doi.org/10.1038/nprot.2008.107	journal	July 2008
Near-equilibrium glycolysis supports metabolic homeostasis and energy yield Park, Junyoung O.; Tanner, Lukas B.; Wei, Monica H. Nature Chemical Biology, Vol. 15, Issue 10 https://doi.org/10.1038/s41589-019-0364-9	journal	September 2019
The discovery of a mevalonate-independent pathway for isoprenoid biosynthesis in bacteria, algae and higher plants† Rohmer, Michel; Rohmer, Michel Natural Product Reports, Vol. 16, Issue 5 https://doi.org/10.1039/a709175c	journal	January 1999
Intracellular crowding defines the mode and sequence of substrate uptake by Escherichia coli and constrains its metabolic activity Beg, Q. K.; Vazquez, A.; Ernst, J. Proceedings of the National Academy of Sciences, Vol. 104, Issue 31 https://doi.org/10.1073/pnas.0609845104	journal	July 2007
Glycolytic strategy as a tradeoff between energy yield and protein cost Flamholz, A.; Noor, E.; Bar-Even, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 24 https://doi.org/10.1073/pnas.1215283110	journal	April 2013
Feedback Inhibition of Deoxy-d-xylulose-5-phosphate Synthase Regulates the Methylerythritol 4-Phosphate Pathway Banerjee, Aparajita; Wu, Yan; Banerjee, Rahul Journal of Biological Chemistry, Vol. 288, Issue 23 https://doi.org/10.1074/jbc.M113.464636	journal	April 2013
Functional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis Hirabayashi, Kei; Yuda, Eiki; Tanaka, Naoyuki Journal of Biological Chemistry, Vol. 290, Issue 50 https://doi.org/10.1074/jbc.M115.680934	journal	October 2015
The SufE Protein and the SufBCD Complex Enhance SufS Cysteine Desulfurase Activity as Part of a Sulfur Transfer Pathway for Fe-S Cluster Assembly in Escherichia coli Outten, F. Wayne; Wood, Matthew J.; Muñoz, F. Michael Journal of Biological Chemistry, Vol. 278, Issue 46 https://doi.org/10.1074/jbc.M308004200	journal	August 2003
Structure and Dynamics of the Isoprenoid Pathway Network Vranová, Eva; Coman, Diana; Gruissem, Wilhelm Molecular Plant, Vol. 5, Issue 2 https://doi.org/10.1093/mp/sss015	journal	March 2012
Isoprene Emission Rate and Intercellular Isoprene Concentration as Influenced by Stomatal Distribution and Conductance Fall, Ray; Monson, Russell K. Plant Physiology, Vol. 100, Issue 2 https://doi.org/10.1104/pp.100.2.987	journal	October 1992
Metabolic Flux Analysis of Plastidic Isoprenoid Biosynthesis in Poplar Leaves Emitting and Nonemitting Isoprene Ghirardo, Andrea; Wright, Louwrance Peter; Bi, Zhen Plant Physiology, Vol. 165, Issue 1 https://doi.org/10.1104/pp.114.236018	journal	March 2014
Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis Wright, Louwrance P.; Rohwer, Johann M.; Ghirardo, Andrea Plant Physiology, Vol. 165, Issue 4 https://doi.org/10.1104/pp.114.245191	journal	July 2014
Zymomonas mobilis as a model system for production of biofuels and biochemicals Yang, Shihui; Fei, Qiang; Zhang, Yaoping Microbial Biotechnology, Vol. 9, Issue 6 https://doi.org/10.1111/1751-7915.12408	journal	September 2016
Biosynthesis of isoprenoids Gabrielsen, Mads; Rohdich, Felix; Eisenreich, Wolfgang European Journal of Biochemistry, Vol. 271, Issue 14 https://doi.org/10.1111/j.1432-1033.2004.04234.x	journal	June 2004
Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing Benjamini, Yoav; Hochberg, Yosef Journal of the Royal Statistical Society: Series B (Methodological), Vol. 57, Issue 1 https://doi.org/10.1111/j.2517-6161.1995.tb02031.x	journal	January 1995
Metabolic engineering for isoprenoid-based biofuel production Gupta, P.; Phulara, S. C. Journal of Applied Microbiology, Vol. 119, Issue 3 https://doi.org/10.1111/jam.12871	journal	July 2015
Manufacturing Molecules Through Metabolic Engineering Keasling, Jay D. Science, Vol. 330, Issue 6009, p. 1355-1358 https://doi.org/10.1126/science.1193990	journal	December 2010
Improving Mobilization of Foreign DNA into Zymomonas mobilis Strain ZM4 by Removal of Multiple Restriction Systems Lal, Piyush Behari; Wells, Fritz; Myers, Kevin S. Applied and Environmental Microbiology, Vol. 87, Issue 19 https://doi.org/10.1128/AEM.00808-21	journal	September 2021
Inducible-Expression Plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans Ind, Alice C.; Porter, Steven L.; Brown, Mostyn T. Applied and Environmental Microbiology, Vol. 75, Issue 20 https://doi.org/10.1128/AEM.01587-09	journal	August 2009
Enhancing Isoprene Production by Genetic Modification of the 1-Deoxy- d -Xylulose-5-Phosphate Pathway in Bacillus subtilis Xue, Junfeng; Ahring, Birgitte K. Applied and Environmental Microbiology, Vol. 77, Issue 7 https://doi.org/10.1128/AEM.02341-10	journal	April 2011
Toward Biosynthetic Design and Implementation of Escherichia coli-Derived Paclitaxel and Other Heterologous Polyisoprene Compounds Jiang, Ming; Stephanopoulos, Gregory; Pfeifer, Blaine A. Applied and Environmental Microbiology, Vol. 78, Issue 8 https://doi.org/10.1128/AEM.07391-11	journal	April 2012
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Glycolytic flux in Zymomonas mobilis: enzyme and metabolite levels during batch fermentation. Osman, Y. A.; Conway, T.; Bonetti, S. J. Journal of Bacteriology, Vol. 169, Issue 8 https://doi.org/10.1128/jb.169.8.3726-3736.1987	journal	January 1987
Content and composition of hopanoids in Zymomonas mobilis under various growth conditions. Hermans, M. A.; Neuss, B.; Sahm, H. Journal of Bacteriology, Vol. 173, Issue 17 https://doi.org/10.1128/jb.173.17.5592-5595.1991	journal	September 1991
Systems-Level Analysis of Oxygen Exposure in Zymomonas mobilis : Implications for Isoprenoid Production Martien, Julia I.; Hebert, Alexander S.; Stevenson, David M. mSystems, Vol. 4, Issue 1 https://doi.org/10.1128/mSystems.00284-18	journal	February 2019
Network Analysis of the MVA and MEP Pathways for Isoprenoid Synthesis Vranová, Eva; Coman, Diana; Gruissem, Wilhelm Annual Review of Plant Biology, Vol. 64, Issue 1 https://doi.org/10.1146/annurev-arplant-050312-120116	journal	April 2013
Zymomonas mobilis: a novel platform for future biorefineries He, Ming; Wu, Bo; Qin, Han Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/1754-6834-7-101	journal	January 2014
Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars Yang, Shihui; Mohagheghi, Ali; Franden, Mary Ann Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0606-y	journal	September 2016
Complete genome sequence and the expression pattern of plasmids of the model ethanologen Zymomonas mobilis ZM4 and its xylose-utilizing derivatives 8b and 2032 Yang, Shihui; Vera, Jessica M.; Grass, Jeff Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1116-x	journal	May 2018
Consistent Estimation of Gibbs Energy Using Component Contributions Noor, Elad; Haraldsdóttir, Hulda S.; Milo, Ron PLoS Computational Biology, Vol. 9, Issue 7 https://doi.org/10.1371/journal.pcbi.1003098	journal	July 2013
Metabolite Profiling Identified Methylerythritol Cyclodiphosphate Efflux as a Limiting Step in Microbial Isoprenoid Production Zhou, Kang; Zou, Ruiyang; Stephanopoulos, Gregory PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0047513	journal	November 2012
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria Farasat, I.; Kushwaha, M.; Collens, J. Molecular Systems Biology, Vol. 10, Issue 6, p. 731-731 https://doi.org/10.15252/msb.20134955	journal	June 2014
Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. Wanke, M.; Skorupinska-Tudek, K.; Swiezewska, E. Acta Biochimica Polonica, Vol. 48, Issue 3 https://doi.org/10.18388/abp.2001_3901	journal	September 2001
Enhancing β-Carotene Production in Escherichia coli by Perturbing Central Carbon Metabolism and Improving the NADPH Supply Wu, Yuanqing; Yan, Panpan; Li, Yang Frontiers in Bioengineering and Biotechnology, Vol. 8 https://doi.org/10.3389/fbioe.2020.00585	journal	June 2020
A Markerless Method for Genome Engineering in Zymomonas mobilis ZM4 Lal, Piyush Behari; Wells, Fritz M.; Lyu, Yucai Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02216	journal	October 2019

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