Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins

Adler, Benjamin A.; Chamakura, Karthik; Carion, Heloise; Krog, Jonathan; Deutschbauer, Adam M.; Young, Ry; Mutalik, Vivek K.; Arkin, Adam P.

doi:10.1038/s41589-023-01269-7

Title: Multicopy suppressor screens reveal convergent evolution of single-gene lysis proteins

Journal Article · Mon Feb 20 00:00:00 EST 2023 · Nature Chemical Biology

DOI:https://doi.org/10.1038/s41589-023-01269-7· OSTI ID:1960236

Adler, Benjamin A. ^[1];

^[2]; Carion, Heloise ^[1]; Krog, Jonathan ^[1];

^[3]; Young, Ry ^[2];

^[4];

^[4]

Univ. of California, Berkeley, CA (United States)
Texas A & M Univ., College Station, TX (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division
Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division

Single-strand RNA (ssRNA) Fiersviridae phages cause host lysis with a product of single gene (sgl for single-gene lysis; product Sgl) that induces autolysis. Many different Sgls have been discovered, but the molecular targets of only a few have been identified. In this study, we used a high-throughput genetic screen to uncover genome-wide host suppressors of diverse Sgls. In addition to validating known molecular mechanisms, we discovered that the Sgl of PP7, an ssRNA phage of Pseudomonas aeruginosa, targets MurJ, the flippase responsible for lipid II export, previously shown to be the target of the Sgl of coliphage M. These two Sgls, which are unrelated and predicted to have opposite membrane topology, thus represent a case of convergent evolution. We extended the genetic screens to other uncharacterized Sgls and uncovered a common set of multicopy suppressors, suggesting that these Sgls act by the same or similar mechanism.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Laboratory Directed Research and Development (LDRD) Program; National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231; R35GM136396; S10RR029668; S10RR027303; OD018174

OSTI ID:: 1960236

Journal Information:: Nature Chemical Biology, Vol. 19, Issue 6; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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