Viral Preservation with Protein-Supplemented Nebulizing Media in Aerosols

Humphrey, Brittany; Tezak, Matthew; Lobitz, Mia; Hendricks, Anastasia; Sanchez, Andres; Zenker, Jake; Storch, Steven; Davis, Ryan D.; Ricken, Bryce; Cahill, Jesse; Buan, ed., Nicole R.

doi:10.1128/aem.01545-22

Viral Preservation with Protein-Supplemented Nebulizing Media in Aerosols

Journal Article · Wed Mar 29 00:00:00 EDT 2023 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.01545-22· OSTI ID:1959268

Humphrey, Brittany ^[1]; Tezak, Matthew ^[1]; Lobitz, Mia ^[1]; Hendricks, Anastasia ^[1]; Sanchez, Andres ^[1]; Zenker, Jake ^[1]; Storch, Steven ^[1]; Davis, Ryan D. ^[1]; Ricken, Bryce ^[1]; ^[1]; Buan, ed., Nicole R.

Sandia National Laboratories, Albuquerque, New Mexico, USA

We have identified common and inexpensive lab reagents that confer increased aerosol survivability on phi6 and other phages. Our results suggest that soluble protein is a key protective component in nebulizing medium.

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Sponsoring Organization:: USDOE

OSTI ID:: 1959268

Alternate ID(s):: OSTI ID: 2311709

Journal Information:: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 3 Vol. 89; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (27)

Review of indoor aerosol generation, transport, and control in the context of COVID‐19 Kohanski, Michael A.; Lo, L. James; Waring, Michael S. International Forum of Allergy & Rhinology, Vol. 10, Issue 10 https://doi.org/10.1002/alr.22661	journal	July 2020
Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2 Mendoza, Emelissa J.; Manguiat, Kathy; Wood, Heidi Current Protocols in Microbiology, Vol. 57, Issue 1 https://doi.org/10.1002/cpmc.105	journal	May 2020
Collection efficiencies of aerosol samplers for virus-containing aerosols Tseng, Chun-Chieh; Li, Chih-Shan Journal of Aerosol Science, Vol. 36, Issue 5-6 https://doi.org/10.1016/j.jaerosci.2004.12.004	journal	May 2005
Inactivation of influenza A viruses in the environment and modes of transmission: A critical review Weber, Thomas P.; Stilianakis, Nikolaos I. Journal of Infection, Vol. 57, Issue 5 https://doi.org/10.1016/j.jinf.2008.08.013	journal	November 2008
Role of Buffers in Protein Formulations Zbacnik, Teddy J.; Holcomb, Ryan E.; Katayama, Derrick S. Journal of Pharmaceutical Sciences, Vol. 106, Issue 3 https://doi.org/10.1016/j.xphs.2016.11.014	journal	March 2017
Susceptibility of an Airborne Common Cold Virus to Relative Humidity Niazi, Sadegh; Groth, Robert; Cravigan, Luke Environmental Science & Technology, Vol. 55, Issue 1 https://doi.org/10.1021/acs.est.0c06197	journal	December 2020
Evaluation of Phi6 Persistence and Suitability as an Enveloped Virus Surrogate Aquino de Carvalho, Nathalia; Stachler, Elyse N.; Cimabue, Nicole Environmental Science & Technology, Vol. 51, Issue 15 https://doi.org/10.1021/acs.est.7b01296	journal	July 2017
Survival of the enveloped bacteriophage Phi6 (a surrogate for SARS-CoV-2) in evaporated saliva microdroplets deposited on glass surfaces Fedorenko, Aliza; Grinberg, Maor; Orevi, Tomer Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-79625-z	journal	December 2020
Transmission of viruses via contact in ahousehold setting: experiments using bacteriophage φX174 as a model virus Rheinbaben, F. v.; Schünemann, S.; Groß, T. Journal of Hospital Infection, Vol. 46, Issue 1 https://doi.org/10.1053/jhin.2000.0794	journal	September 2000
TinyLev: A multi-emitter single-axis acoustic levitator Marzo, Asier; Barnes, Adrian; Drinkwater, Bruce W. Review of Scientific Instruments, Vol. 88, Issue 8 https://doi.org/10.1063/1.4989995	journal	August 2017
Influence of organic films on the evaporation and condensation of water in aerosol Davies, J. F.; Miles, R. E. H.; Haddrell, A. E. Proceedings of the National Academy of Sciences, Vol. 110, Issue 22 https://doi.org/10.1073/pnas.1305277110	journal	May 2013
Evidence for a semisolid phase state of aerosols and droplets relevant to the airborne and surface survival of pathogens Huynh, Erik; Olinger, Anna; Woolley, David Proceedings of the National Academy of Sciences, Vol. 119, Issue 4 https://doi.org/10.1073/pnas.2109750119	journal	January 2022
The dynamics of SARS-CoV-2 infectivity with changes in aerosol microenvironment Oswin, Henry P.; Haddrell, Allen E.; Otero-Fernandez, Mara Proceedings of the National Academy of Sciences, Vol. 119, Issue 27 https://doi.org/10.1073/pnas.2200109119	journal	June 2022
Assessing suspension and infectivity times of virus-loaded aerosols involved in airborne transmission Merhi, Tania; Atasi, Omer; Coetsier, Clémence Proceedings of the National Academy of Sciences, Vol. 119, Issue 32 https://doi.org/10.1073/pnas.2204593119	journal	August 2022
Spread of viral infections by aerosols Sattar, Syed A.; Ijaz, Mohammad Khalid Critical Reviews in Environmental Control, Vol. 17, Issue 2 https://doi.org/10.1080/10643388709388331	journal	January 1987
The effect of environmental parameters on the survival of airborne infectious agents Tang, Julian W. Journal of The Royal Society Interface, Vol. 6, Issue suppl_6 https://doi.org/10.1098/rsif.2009.0227.focus	journal	September 2009
Physico-chemical characteristics of evaporating respiratory fluid droplets Vejerano, Eric P.; Marr, Linsey C. Journal of The Royal Society Interface, Vol. 15, Issue 139 https://doi.org/10.1098/rsif.2017.0939	journal	February 2018
In situ measurements of human cough aerosol hygroscopicity Groth, Robert; Cravigan, Luke T.; Niazi, Sadegh Journal of The Royal Society Interface, Vol. 18, Issue 178 https://doi.org/10.1098/rsif.2021.0209	journal	May 2021
Survival of Airborne MS2 Bacteriophage Generated from Human Saliva, Artificial Saliva, and Cell Culture Medium Zuo, Zhili; Kuehn, Thomas H.; Bekele, Aschalew Z. Applied and Environmental Microbiology, Vol. 80, Issue 9 https://doi.org/10.1128/AEM.00056-14	journal	May 2014
Effects of Relative Humidity and Spraying Medium on UV Decontamination of Filters Loaded with Viral Aerosols Woo, Myung-Heui; Grippin, Adam; Anwar, Diandra Applied and Environmental Microbiology, Vol. 78, Issue 16 https://doi.org/10.1128/AEM.00465-12	journal	August 2012
Survival of the Enveloped Virus Phi6 in Droplets as a Function of Relative Humidity, Absolute Humidity, and Temperature Prussin, Aaron J.; Schwake, David Otto; Lin, Kaisen Applied and Environmental Microbiology, Vol. 84, Issue 12 https://doi.org/10.1128/AEM.00551-18	journal	June 2018
Comparison of Five Bacteriophages as Models for Viral Aerosol Studies Turgeon, Nathalie; Toulouse, Marie-Josée; Martel, Bruno Applied and Environmental Microbiology, Vol. 80, Issue 14 https://doi.org/10.1128/AEM.00767-14	journal	July 2014
Mechanisms by Which Ambient Humidity May Affect Viruses in Aerosols Yang, Wan; Marr, Linsey C. Applied and Environmental Microbiology, Vol. 78, Issue 19 https://doi.org/10.1128/AEM.01658-12	journal	October 2012
Survival of coxsackievirus B3 under diverse environmental conditions McGeady, M. L.; Siak, J. S.; Crowell, R. L. Applied and Environmental Microbiology, Vol. 37, Issue 5 https://doi.org/10.1128/aem.37.5.972-977.1979	journal	May 1979
Inactivation of Baceriophage T ₃ in Aerosols: Effect of Prehumidification on Survival After Spraying from Solutions of Salt, Peptone, and Saliva Trouwborst, T.; Kuyper, Sjoukje Applied Microbiology, Vol. 27, Issue 5 https://doi.org/10.1128/am.27.5.834-837.1974	journal	May 1974
Survival of MS2 and Φ6 viruses in droplets as a function of relative humidity, pH, and salt, protein, and surfactant concentrations Lin, Kaisen; Schulte, Chase R.; Marr, Linsey C. PLOS ONE, Vol. 15, Issue 12 https://doi.org/10.1371/journal.pone.0243505	journal	December 2020
Bacteriophages Adams, Mark Hancock https://doi.org/10.5962/bhl.title.6966	journal	January 1959

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