Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]

Mageeney, Catherine M.; Mohammed, Hamidu T.; Dies, Marta; Anbari, Samira; Cudkevich, Netta; Chen, Yanyan; Buceta, Javier; Ware, Vassie C.

doi:10.1128/msystems.00534-20

Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack [plus supplemental information]

Journal Article · Tue Oct 06 00:00:00 EDT 2020 · mSystems

DOI:https://doi.org/10.1128/msystems.00534-20· OSTI ID:1668358

Mageeney, Catherine M. ^[1]; Mohammed, Hamidu T. ^[2]; Dies, Marta ^[2]; Anbari, Samira ^[2]; Cudkevich, Netta ^[2]; Chen, Yanyan ^[2]; Buceta, Javier ^[3]; ^[2]

Lehigh Univ., Bethlehem, PA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Lehigh Univ., Bethlehem, PA (United States)
Lehigh Univ., Bethlehem, PA (United States); Institute for Integrative Systems Biology (I2SysBio), CSIC-UV, Paterna, Valencia (Spain)

A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc²155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analyses, phage plating efficiency experiments, microscopy, and immunoprecipitation assays, we show that Butters genes located in the central region of the genome play a key role in the defense against heterotypic viral attack. Our study suggests that a two-component system, articulated by interactions between protein products of genes 30 and 31, confers defense against heterotypic phage infection by PurpleHaze (cluster A/subcluster A3) or Alma (cluster A/subcluster A9) but is insufficient to confer defense against attack by the heterotypic phage Island3 (cluster I/subcluster I1). Therefore, based on heterotypic phage plating efficiencies on the Butters lysogen, additional prophage genes required for defense are implicated and further show specificity of prophage-encoded defense systems. IMPORTANCE: Many sequenced bacterial genomes, including those of pathogenic bacteria, contain prophages. Some prophages encode defense systems that protect their bacterial host against heterotypic viral attack. Understanding the mechanisms undergirding these defense systems is crucial to appreciate the scope of bacterial immunity against viral infections and will be critical for better implementation of phage therapy that would require evasion of these defenses. Furthermore, such knowledge of prophage-encoded defense mechanisms may be useful for developing novel genetic tools for engineering phage-resistant bacteria of industrial importance.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE; Pennsylvania Department of Community and Economic Development; Lehigh University

Grant/Contract Number:: AC04-94AL85000

OSTI ID:: 1668358

Report Number(s):: SAND--2020-5059J; 686079

Journal Information:: mSystems, Journal Name: mSystems Journal Issue: 5 Vol. 5; ISSN 2379-5077

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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