Evaluation of the metabolic fate of munitions material (TNT & RDX) in plant systems and initial assessment of material interaction with plant genetic material (DNA). Initial assessment of plant DNA adducts as biomarkers
Genetic damage to deoxyribonucleic acid (DNA) has long been suspected of being a fundamental event leading to cancer. A variety of causal factors can result in DNA damage including photodimerization of base pairs, ionizing radiation, specific reaction of DNA with environmental pollutants, and nonspecific oxidative damage caused by the action of highly reactive oxidizing agents produced by metabolism. Because organisms depend on an unadulterated DNA template for reproduction, DNA repair mechanisms are an important defense for maintaining genomic integrity. The objective of this exploratory project was to evaluate the potential for TNT to form DNA adducts in plants. These adducts, if they exist in sufficient quantities, could be potential biomarkers of munitions exposure. The ultimate goal is to develop a simple analytical assay for the determination of blomarkers that is indicative of munitions contamination. DNA repair exists in dynamic equilibrium with DNA damage. Repair mechanisms are capable of keeping DNA damage at remarkably low concentrations provided that the repair capacity is not overwhelmed.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (United States)
- Sponsoring Organization:
- Department of Defense, Washington, DC (United States)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 195771
- Report Number(s):
- PNL--10738; ON: DE96003998
- Country of Publication:
- United States
- Language:
- English
Similar Records
Evaluation of the metabolic fate of munitions material (TNT & RDX) in plant systems. Initial assessment of plant DNA mutation spectra as a biomarker
Evaluation of the metabolic fate of munitions material (TNT & RDX) in plant systems and initial assessment of material interaction with plant genetic material. Validation of the metabolic fate of munitions materials (TNT, RDX) in mature crops
Ozonation of DNA forms adducts: A [sup 32]P-DNA labeling and thin-layer chromatography technique to measure DNA environmental biomarkers
Technical Report
·
Fri Sep 01 00:00:00 EDT 1995
·
OSTI ID:187260
Evaluation of the metabolic fate of munitions material (TNT & RDX) in plant systems and initial assessment of material interaction with plant genetic material. Validation of the metabolic fate of munitions materials (TNT, RDX) in mature crops
Technical Report
·
Fri Sep 01 00:00:00 EDT 1995
·
OSTI ID:187259
Ozonation of DNA forms adducts: A [sup 32]P-DNA labeling and thin-layer chromatography technique to measure DNA environmental biomarkers
Journal Article
·
· Archives of Environmental Health; (United States)
·
OSTI ID:7284329