Preparative and high efficiency separation of fullerenes
- Univ. of Missouri, Rolla, MO (United States)
The number of high performance liquid chromatography (HPLC) stationary phases for the separation and purification of fullerenes has increased tremendously over the past few years. Several phases were developed solely for the purpose of separating fullerenes some of which are very costly. Most of the stationary phases available give a more than adequate separation between C{sub 60} and C{sub 70} provided the optimum mobile phase is used. Duplication, overlap, and several discrepancies exist when comparing the selectivities of these columns. In this study, the selectivity, resolution, column loadability, and higher fullerene isolation of several commercial aliphatic and aromatic stationary phases were compared. The demand for larger quantities of fullerenes has led to the challenge of separating fullerenes on a preparative basis. Unfortunately, fullerenes have limited solubilities in most HPLC eluents. Recently, over 100 binary, ternary, or quaternary organic systems were prescreened on a small 5 ml scale. In several immiscible systems, the partition coefficients of C{sub 60} and C{sub 70}, were determined. With this information, a viable two phase organic system was developed which separates large quantities of fullerenes using centrifugal partition chromatography (CPC). CPC is a preparative chromatographic technique that utilizes the principles of countercurrent chromatography and liquid-liquid extraction. Over 100 mg of a saturated solution of fullerenes per injection (i.e., a 100-fold increase in the amount purified on previous HPLC stationary phases) can be separated using this methodology.
- OSTI ID:
- 191764
- Report Number(s):
- CONF-941098--
- Country of Publication:
- United States
- Language:
- English
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