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Title: Highly multiplexed DNA sequencing by capillary electrophoresis

Abstract

It is obvious that irrespective of whichever basic technology is eventually selected to sequence the entire human genome there are substantial gains to be made if a high degree of multiplexing of parallel runs can be implemented. Such multiplexing should not involve expensive instrumentation and should not require additional personnel, or else the main objective of cost reduction will not be satisfied even though the total time for sequencing is reduced. In the last two years, several research groups have shown that capillary electrophoresis (CE) is an attractive alternative for DNA sequencing. Part of the improvement in sequencing speed in CE is counteracted by the inherent ability of slab gels for accommodating multiple lanes in a single run. Recently, the authors have developed several excitation schemes for highly multiplexed capillary electrophoresis. Detection at the pM level was demonstrated. The authors report here the use of a novel excitation geometry to simultaneously monitor 100 capillary tubes during electrophoresis. This represents a truly parallel multiplexing scheme for high-speed DNA sequencing.

Authors:
; ;  [1]
  1. Iowa State Univ., Ames, IA (United States); and others
Publication Date:
OSTI Identifier:
191742
Report Number(s):
CONF-941098-
TRN: 95:006733-0098
Resource Type:
Conference
Resource Relation:
Conference: FACSS XXI: 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), St. Louis, MO (United States), 2-7 Oct 1994; Other Information: PBD: 1994; Related Information: Is Part Of 21st annual conference of the Federation of Analytical Chemistry and Spectroscopy Societies; PB: 257 p.
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCING; OPTIMIZATION; ELECTROPHORESIS; COST

Citation Formats

Yeung, E S, Ueno, K, and Chang, H T. Highly multiplexed DNA sequencing by capillary electrophoresis. United States: N. p., 1994. Web.
Yeung, E S, Ueno, K, & Chang, H T. Highly multiplexed DNA sequencing by capillary electrophoresis. United States.
Yeung, E S, Ueno, K, and Chang, H T. 1994. "Highly multiplexed DNA sequencing by capillary electrophoresis". United States.
@article{osti_191742,
title = {Highly multiplexed DNA sequencing by capillary electrophoresis},
author = {Yeung, E S and Ueno, K and Chang, H T},
abstractNote = {It is obvious that irrespective of whichever basic technology is eventually selected to sequence the entire human genome there are substantial gains to be made if a high degree of multiplexing of parallel runs can be implemented. Such multiplexing should not involve expensive instrumentation and should not require additional personnel, or else the main objective of cost reduction will not be satisfied even though the total time for sequencing is reduced. In the last two years, several research groups have shown that capillary electrophoresis (CE) is an attractive alternative for DNA sequencing. Part of the improvement in sequencing speed in CE is counteracted by the inherent ability of slab gels for accommodating multiple lanes in a single run. Recently, the authors have developed several excitation schemes for highly multiplexed capillary electrophoresis. Detection at the pM level was demonstrated. The authors report here the use of a novel excitation geometry to simultaneously monitor 100 capillary tubes during electrophoresis. This represents a truly parallel multiplexing scheme for high-speed DNA sequencing.},
doi = {},
url = {https://www.osti.gov/biblio/191742}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1994},
month = {12}
}

Conference:
Other availability
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