Ru(bpy){sup 3+}{sub 3} - based detection of underivatized amino acids separated by capillary electrophoresis
- Univ. of Arkansas, Fayetteville, AR (United States); and others
Recent reports have suggested that improvements are needed in the ability to sequence proteins of one to two orders-of-magnitude in order to continue advances in the areas of molecular biology and biomedical research. This talk will focus on a recently developed chemiluminescent detection technique for the analysis of underivatized amino acids in which the amino acid is reacted with tris(2,2`-bipyridyl)ruthenium(III) yielding significant chemiluminescent emission as a product of the reaction. The technology has been applied as a detection mode for capillary electrophoresis. Underivatized amino acids can be separated by CE and then sensitively detected using the Ru{sup 3+} based detection system. A carbon microelectrode positioned at the elution end of the capillary provides a source of Ru{sup 3+} through the oxidation of Ru{sup 2+}. The Ru{sup 2+} can be present in the CE buffer or added post-capillary. This presentation will focus on the chemical and instrumental parameters necessary to detect underivatized amino acids at the femtomole level, and a general strategy will be discussed suggesting how this approach can be incorporated into existing sequencing protocols.
- OSTI ID:
- 191722
- Report Number(s):
- CONF-941098--
- Country of Publication:
- United States
- Language:
- English
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