Analyzing the Modification of the Shewanella oneidensis MR-1 Flagellar Filament

Bubendorfer, Sebastian; Ishihara, Mayumi; Dohlich, Kim; Heiss, Christian; Vogel, Jan; Sastre, Federico; Panico, Maria; Hitchen, Paul; Dell, Anne; Azadi, Parastoo; Thormann, Kai M.

doi:10.1371/journal.pone.0073444

Analyzing the Modification of the Shewanella oneidensis MR-1 Flagellar Filament

Journal Article · Fri Sep 06 00:00:00 EDT 2013 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0073444· OSTI ID:1904588

Bubendorfer, Sebastian ^[1]; Ishihara, Mayumi ^[2]; Dohlich, Kim ^[3]; Heiss, Christian ^[2]; Vogel, Jan ^[4]; Sastre, Federico ^[5]; Panico, Maria ^[6]; Hitchen, Paul ^[6]; Dell, Anne ^[6]; Azadi, Parastoo ^[2]; Thormann, Kai M. ^[4]

Max Planck Institute for Terrestrial Microbiology, Marburg (Germany); Max Planck Inst. for Terrestrial Microbiology, Marburg (Germany)
University of Georgia, Athens, GA (United States)
Max Planck Institute for Infection Biology, Berlin (Germany)
Max Planck Institute for Terrestrial Microbiology, Marburg (Germany)
Imperial College, London (United Kingdom); Pontificia Universidad Catolica de Chile, Santiago (Chile)
Imperial College, London (United Kingdom)

The unsheathed flagellar filament of Shewanella oneidensis MR-1 is composed of two highly homologous flagellins, FlaA, and the major structural unit, FlaB. We identified a gene cluster, SO_3261-SO_3265 (now sfmABCDE), that is required for the formation of a fully functional filament and for motility. The predicted function of the corresponding gene products strongly indicated a role in flagellin modification. Accordingly, loss of sfmABCDE results in a significant mass shift of both FlaA and FlaB. Mass spectroscopy analysis and single residue substitutions identified five serine residues in both flagellins that are modified via O-linkage. Modeling of the flagellin structures strongly suggests that at least four of the modified residues are exposed to the filament’s surface. However, none of the five serine residues solely is crucial for function and assembly. Structural analysis of the flagellin modification revealed that it likely contains a nonulosonic acid (274 Da) linked to each glycosylated serine. The putative nonulosonic acid is further substituted with a 236 Da moiety which can carry additional methyl groups (250 Da, 264 Da). In addition, at least 5 lysine residues in FlaB and one in FlaA were found to be methylated. Based on homology comparisons we suggest that smfABCDE is required for species-specific flagellin modification in S. oneidensis MR-1.

View Accepted Manuscript (DOE)

Research Organization:: University of Georgia, Athens, GA (United States)

Sponsoring Organization:: Biotechnology and Biological Sciences Research Council; Max-Planck-Gesellschaft (MPG); National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1904588

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 9 Vol. 8; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Analyzing the Modification of the Shewanella oneidensis MR-1 Flagellar Filament

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