Identification of a pathway for electron uptake in Shewanella oneidensis

Rowe, Annette R.; Salimijazi, Farshid; Trutschel, Leah; Sackett, Joshua; Adesina, Oluwakemi; Anzai, Isao; Kugelmass, Liat H.; Baym, Michael H.; Barstow, Buz

doi:10.1038/s42003-021-02454-x

Identification of a pathway for electron uptake in Shewanella oneidensis

Journal Article · Wed Aug 11 00:00:00 EDT 2021 · Communications Biology

DOI:https://doi.org/10.1038/s42003-021-02454-x· OSTI ID:1853493

^[1]; ^[2]; Trutschel, Leah ^[3]; Sackett, Joshua ^[3]; Adesina, Oluwakemi ^[4]; Anzai, Isao ^[4]; Kugelmass, Liat H. ^[4]; ^[5]; ^[2]

Univ. of Cincinnati, OH (United States); Cornell Univ., Ithaca, NY (United States)
Cornell Univ., Ithaca, NY (United States)
Univ. of Cincinnati, OH (United States)
Princeton Univ., NJ (United States)
Harvard Medical School, Boston, MA (United States)

Extracellular electron transfer (EET) could enable electron uptake into microbial metabolism for the synthesis of complex, energy dense organic molecules from CO₂ and renewable electricity. Theoretically EET could do this with an efficiency comparable to H₂-oxidation but without the need for a volatile intermediate and the problems it causes for scale up. However, significant gaps remain in understanding the mechanism and genetics of electron uptake. For example, studies of electron uptake in electroactive microbes have shown a role for the Mtr EET complex in the electroactive microbe Shewanella oneidensis MR-1, though there is substantial variation in the magnitude of effect deletion of these genes has depending on the terminal electron acceptor used. This speaks to the potential for previously uncharacterized and/or differentially utilized genes involved in electron uptake. To address this, we screened gene disruption mutants for 3667 genes, representing ≈99% of all nonessential genes, from the S. oneidensis whole genome knockout collection using a redox dye oxidation assay. Confirmation of electron uptake using electrochemical testing allowed us to identify five genes from S. oneidensis that are indispensable for electron uptake from a cathode. Knockout of each gene eliminates extracellular electron uptake, yet in four of the five cases produces no significant defect in electron donation to an anode. This result highlights both distinct electron uptake components and an electronic connection between aerobic and anaerobic electron transport chains that allow electrons from the reversible EET machinery to be coupled to different respiratory processes in S. oneidensis. Homologs to these genes across many different genera suggesting that electron uptake by EET coupled to respiration could be widespread. These gene discoveries provide a foundation for: studying this phenotype in exotic metal-oxidizing microbes, genetic optimization of electron uptake in S. oneidensis; and genetically engineering electron uptake into a highly tractable host like E. coli to complement recent advances in synthetic CO2 fixation.

View Accepted Manuscript (DOE)

Research Organization:: Cornell Univ., Ithaca, NY (United States); Univ. of Cincinnati, OH (United States)

Sponsoring Organization:: Air Force Office of Scientific Research (AFOSR); Burroughs Welcome Fund; Cornell University; Princeton University; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0020179

OSTI ID:: 1853493

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 4; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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