Soluble Iron Enhances Extracellular Electron Uptake by Shewanella oneidensis MR‐1

Abuyen, Karla; El‐Naggar, Mohamed Y.

doi:10.1002/celc.202200965

Title: Soluble Iron Enhances Extracellular Electron Uptake by Shewanella oneidensis MR‐1

Journal Article · Wed Jan 11 00:00:00 EST 2023 · ChemElectroChem

DOI:https://doi.org/10.1002/celc.202200965· OSTI ID:1908877

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Molecular and Computational Biology Section Department of Biological Sciences University of Southern California Los Angeles CA 90089 USA) Website
Molecular and Computational Biology Section Department of Biological Sciences University of Southern California Los Angeles CA 90089 USA) Website, Department of Physics and Astronomy University of Southern California Los Angeles CA 90089 USA, Department of Chemistry University of Southern California Los Angeles CA 90089 USA

Abstract Extracellular electron transfer (EET) is a process that microorganisms use to reduce or oxidize external insoluble electron acceptors or donors. Much of our mechanistic understanding of this process is derived from studies of transmembrane cytochrome complexes and extracellular redox shuttles that mediate outward EET to anodes and external electron acceptors. In contrast, there are knowledge gaps concerning the reverse process of inward EET from external electron donors to cells. Here, we describe a role for soluble iron (exogenous FeCl ₂ ) in enhancing EET from cathodes to the model EET bacterium Shewanella oneidensis MR‐1, with fumarate serving as the intracellular electron acceptor. This iron concentration‐dependent electron uptake was eradicated upon addition of an iron chelator and occurred only in the presence of fumarate reductase, confirming an electron pathway from cathodes to this periplasmic enzyme. Moreover, S. oneidensis mutants lacking specific outer membrane and periplasmic cytochromes exhibited significantly decreased current levels relative to wild‐type. These results indicate that soluble iron can function as an electron carrier to the EET machinery of S. oneidensis .

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-13ER16415; SC0010609

OSTI ID:: 1908877

Alternate ID(s):: OSTI ID: 1924829

Journal Information:: ChemElectroChem, Journal Name: ChemElectroChem Vol. 10 Journal Issue: 4; ISSN 2196-0216

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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