SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet–plasma membrane tethering

Krawczyk, Hannah Elisa; Sun, Siqi; Doner, Nathan M.; Yan, Qiqi; Lim, Magdiel Sheng Satha; Scholz, Patricia; Niemeyer, Philipp William; Schmitt, Kerstin; Valerius, Oliver; Pleskot, Roman; Hillmer, Stefan; Braus, Gerhard H.; Wiermer, Marcel; Mullen, Robert T.; Ischebeck, Till

doi:10.1093/plcell/koac095

SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet–plasma membrane tethering

Journal Article · Mon Mar 28 00:00:00 EDT 2022 · The Plant Cell

DOI:https://doi.org/10.1093/plcell/koac095· OSTI ID:1904415

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University of Gottingen (Germany); OSTI
University of Gottingen (Germany)
Univ. of Guelph, ON (Canada)
Czech Academy of Sciences (CAS), Prague (Czech Republic). Institute of Experimental Botany
Heidelberg Univ. (Germany)
University of Gottingen (Germany); Univ. of Munster (Germany)

Membrane contact sites (MCSs) are interorganellar connections that allow for the direct exchange of molecules, such as lipids or Ca²⁺ between organelles, but can also serve to tether organelles at specific locations within cells. Here, we identified and characterized three proteins of Arabidopsis thaliana that form a lipid droplet (LD)–plasma membrane (PM) tethering complex in plant cells, namely LD-localized SEED LD PROTEIN (SLDP) 1 and SLDP2 and PM-localized LD-PLASMA MEMBRANE ADAPTOR (LIPA). Using proteomics and different protein–protein interaction assays, we show that both SLDPs associate with LIPA. Disruption of either SLDP1 and SLDP2 expression, or that of LIPA, leads to an aberrant clustering of LDs in Arabidopsis seedlings. Ectopic co-expression of one of the SLDPs with LIPA is sufficient to reconstitute LD–PM tethering in Nicotiana tabacum pollen tubes, a cell type characterized by dynamically moving LDs in the cytosolic streaming. Furthermore, confocal laser scanning microscopy revealed both SLDP2.1 and LIPA to be enriched at LD–PM contact sites in seedlings. These and other results suggest that SLDP and LIPA interact to form a tethering complex that anchors a subset of LDs to the PM during post-germinative seedling growth in Arabidopsis.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); German Research Foundation (DFG); Natural Sciences and Engineering Research Council of Canada (NSERC)

Grant/Contract Number:: SC0016536

OSTI ID:: 1904415

Journal Information:: The Plant Cell, Journal Name: The Plant Cell Journal Issue: 6 Vol. 34; ISSN 1040-4651

Publisher:: American Society of Plant Biologists (ASPB)Copyright Statement

Country of Publication:: United States

Language:: English

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LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis

Journal Article · Wed Jun 09 00:00:00 EDT 2021 · The Plant Cell · OSTI ID:1852282

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59 BASIC BIOLOGICAL SCIENCES
Biochemistry & Molecular Biology
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Plant Sciences

SEED LIPID DROPLET PROTEIN1, SEED LIPID DROPLET PROTEIN2, and LIPID DROPLET PLASMA MEMBRANE ADAPTOR mediate lipid droplet–plasma membrane tethering

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