Adaptive responses of marine diatoms to zinc scarcity and ecological implications

Kellogg, Riss M.; Moosburner, Mark A.; Cohen, Natalie R.; Hawco, Nicholas J.; McIlvin, Matthew R.; Moran, Dawn M.; DiTullio, Giacomo R.; Subhas, Adam V.; Allen, Andrew E.; Saito, Mak A.

doi:10.1038/s41467-022-29603-y

Title: Adaptive responses of marine diatoms to zinc scarcity and ecological implications

Journal Article · Thu Apr 14 00:00:00 EDT 2022 · Nature Communications

DOI:https://doi.org/10.1038/s41467-022-29603-y· OSTI ID:1904389

^[1]; Moosburner, Mark A. ^[2];

^[3];

^[4]; McIlvin, Matthew R. ^[5]; Moran, Dawn M. ^[5];

^[6]; Subhas, Adam V. ^[5];

^[2];

^[5]

Woods Hole Oceanographic Institution, MA (United States)
J. Craig Venter Institute, La Jolla, CA (United States); University of California, San Diego, CA (United States). Scripps Institute of Oceanography
Woods Hole Oceanographic Institution, Woods Hole, MA (United States); Univ. of Georgia, Savannah, GA (United States)
University of Hawaii at Manoa, Honolulu, HI (United States)
Woods Hole Oceanographic Institution, Woods Hole, MA (United States)
College of Charleston, SC (United States)

Scarce dissolved surface ocean concentrations of the essential algal micronutrient zinc suggest that Zn may influence the growth of phytoplankton such as diatoms, which are major contributors to marine primary productivity. However, the specific mechanisms by which diatoms acclimate to Zn deficiency are poorly understood. Using global proteomic analysis, we identified two proteins (ZCRP-A/B, Zn/Co Responsive Protein A/B) among four diatom species that became abundant under Zn/Co limitation. Characterization using reverse genetic techniques and homology data suggests putative Zn/Co chaperone and membrane-bound transport complex component roles for ZCRP-A (a COG0523 domain protein) and ZCRP-B, respectively. Metaproteomic detection of ZCRPs along a Pacific Ocean transect revealed increased abundances at the surface (<200 m) where dZn and dCo were scarcest, implying Zn nutritional stress in marine algae is more prevalent than previously recognized. These results demonstrate multiple adaptive responses to Zn scarcity in marine diatoms that are deployed in low Zn regions of the Pacific Ocean.

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Research Organization:: J. Craig Venter Institute, Inc., Rockville, MD (United States); Woods Hole Oceanographic Institution, Woods Hole, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); National Institutes of Health (NIH); Gordon and Betty Moore Foundation; Simons Foundation

Grant/Contract Number:: SC0018344; OCE-1736599; OCE-1657766; R01GM135709; GBMF3782; 544236; OCE-1756884; MCB-1818390; GBMF3828; GBMF5006

OSTI ID:: 1904389

Journal Information:: Nature Communications, Vol. 13, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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