Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha

Dierschke, Tom; Flores-Sandoval, Eduardo; Rast-Somssich, Madlen I.; Althoff, Felix; Zachgo, Sabine; Bowman, John L.

doi:10.7554/elife.57088

Title: Gamete expression of TALE class HD genes activates the diploid sporophyte program in Marchantia polymorpha

Journal Article · Fri Sep 17 00:00:00 EDT 2021 · eLife

DOI:https://doi.org/10.7554/elife.57088· OSTI ID:1904155

^[1]; Flores-Sandoval, Eduardo ^[2]; Rast-Somssich, Madlen I. ^[2]; Althoff, Felix ^[3]; Zachgo, Sabine ^[3];

^[2]

Monash University, Melbourne, VIC (Australia); University of Osnabruck (Germany)
Monash University, Melbourne, VIC (Australia)
University of Osnabruck (Germany)

Eukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga Chlamydomonas, KNOX and BELL TALE-homeodomain genes mediate this transition. We demonstrate that in the liverwort Marchantia polymorpha, paternal (sperm) expression of three of five phylogenetically diverse BELL genes, MpBELL234, and maternal (egg) expression of both MpKNOX1 and MpBELL34 mediate the haploid-to-diploid transition. Loss-of-function alleles of MpKNOX1 result in zygotic arrest, whereas a loss of either maternal or paternal MpBELL234 results in variable zygotic and early embryonic arrest. Expression of MpKNOX1 and MpBELL34 during diploid sporophyte development is consistent with a later role for these genes in patterning the sporophyte. These results indicate that the ancestral mechanism to activate diploid gene expression was retained in early diverging land plants and subsequently co-opted during evolution of the diploid sporophyte body.

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Research Organization:: USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Monash Univ., Melbourne, VIC (Australia)

Sponsoring Organization:: USDOE Office of Science (SC); Australian Research Council; German Research Foundation (DFG)

Grant/Contract Number:: AC02-05CH11231; FF0551326; DP130100177; DP170100049; DP210101423; SFB944, P13

OSTI ID:: 1904155

Journal Information:: eLife, Vol. 10; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: English

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