Structural features of Dnase1L3 responsible for serum antigen clearance

McCord, Jon J.; Engavale, Minal; Masoumzadeh, Elahe; Villarreal, Johanna; Mapp, Britney; Latham, Michael P.; Keyel, Peter A.; Sutton, R. Bryan

doi:10.1038/s42003-022-03755-5

Structural features of Dnase1L3 responsible for serum antigen clearance

Journal Article · Tue Aug 16 00:00:00 EDT 2022 · Communications Biology

DOI:https://doi.org/10.1038/s42003-022-03755-5· OSTI ID:1903977

^[1]; Engavale, Minal ^[2]; Masoumzadeh, Elahe ^[3]; Villarreal, Johanna ^[4]; Mapp, Britney ^[2]; ^[3]; ^[2]; ^[4]

Texas Tech Univ., Lubbock, TX (United States). Health Sciences Center. Dept. of Cell Physiology and Molecular Biophysics; OSTI
Texas Tech Univ., Lubbock, TX (United States). Dept. of Biological Sciences
Texas Tech Univ., Lubbock, TX (United States). Dept. of Chemistry & Biochemistry
Texas Tech Univ., Lubbock, TX (United States). Health Sciences Center. Dept. of Cell Physiology and Molecular Biophysics

Autoimmunity develops when extracellular DNA released from dying cells is not cleared from serum. While serum DNA is primarily digested by Dnase1 and Dnase1L3, Dnase1 cannot rescue autoimmunity arising from Dnase1L3 deficiencies. Dnase1L3 uniquely degrades antigenic forms of cell-free DNA, including DNA complexed with lipids and proteins. The distinct activity of Dnase1L3 relies on its unique C-terminal Domain (CTD), but the mechanism is unknown. We used multiple biophysical techniques and functional assays to study the interplay between the core catalytic domain and the CTD. While the core domain resembles Dnase1, there are key structural differences between the two enzymes. First, Dnase1L3 is not inhibited by actin due to multiple differences in the actin recognition site. Second, the CTD augments the ability of the core to bind DNA, thereby facilitating the degradation of complexed DNA. Together, these structural insights will inform the development of Dnase1L3-based therapies for autoimmunity.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1903977

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 5; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

References (71)

DNASE1L3Mutations in Hypocomplementemic Urticarial Vasculitis Syndrome Özçakar, Z. Birsin; Foster, Joseph; Diaz-Horta, Oscar Arthritis & Rheumatism, Vol. 65, Issue 8 https://doi.org/10.1002/art.38010	journal	July 2013
Taming hyperactive hDNase I: Stable inducible expression of a hyperactive salt- and actin-resistant variant of human deoxyribonuclease I in CHO cells Lam, Cynthia; Santell, Lydia; Wilson, Blair Biotechnology Progress, Vol. 33, Issue 2 https://doi.org/10.1002/btpr.2439	journal	February 2017
Comparative Protein Structure Modeling Using MODELLER Webb, Benjamin; Sali, Andrej Current Protocols in Bioinformatics, Vol. 54, Issue 1 https://doi.org/10.1002/cpbi.3	journal	June 2016
CHARMM-GUI: A web-based graphical user interface for CHARMM Jo, Sunhwan; Kim, Taehoon; Iyer, Vidyashankara G. Journal of Computational Chemistry, Vol. 29, Issue 11 https://doi.org/10.1002/jcc.20945	journal	March 2008
Mutational analysis of human DNase I at the DNA binding interface: Implications for DNA recognition, catalysis, and metal ion dependence Pan, Clark Q.; Uumer, Jana S.; Herzka, Andrea Protein Science, Vol. 7, Issue 3 https://doi.org/10.1002/pro.5560070312	journal	March 1998
The CCPN data model for NMR spectroscopy: Development of a software pipeline Vranken, Wim F.; Boucher, Wayne; Stevens, Tim J. Proteins: Structure, Function, and Bioinformatics, Vol. 59, Issue 4 https://doi.org/10.1002/prot.20449	journal	April 2005
A simple clustering algorithm can be accurate enough for use in calculations of pKs in macromolecules Myers, Jonathan; Grothaus, Greg; Narayanan, Shivaram Proteins: Structure, Function, and Bioinformatics, Vol. 63, Issue 4 https://doi.org/10.1002/prot.20922	journal	February 2006
DelPhiPKa: Including salt in the calculations and enabling polar residues to titrate Pahari, Swagata; Sun, Lexuan; Basu, Sankar Proteins: Structure, Function, and Bioinformatics, Vol. 86, Issue 12 https://doi.org/10.1002/prot.25608	journal	October 2018
Characterizing the Use of Perdeuteration in NMR Studies of Large Proteins:13C,15N and1H Assignments of Human Carbonic Anhydrase II Venters, Ronald A.; Farmer II, Bennett T.; Fierke, Carol A. Journal of Molecular Biology, Vol. 264, Issue 5 https://doi.org/10.1006/jmbi.1996.0699	journal	December 1996
Site-directed Mutagenesis of the Catalytic Residues of Bovine Pancreatic Deoxyribonuclease I Jones, Stephen J.; Worrall, Andrew F.; Connolly, Bernard A. Journal of Molecular Biology, Vol. 264, Issue 5 https://doi.org/10.1006/jmbi.1996.0703	journal	December 1996
Deoxyribonuclease I-like III Is an Inducible Macrophage Barrier to Liposomal Transfection Wilber, Andrew; Lu, Michael; Schneider, Michael C. Molecular Therapy, Vol. 6, Issue 1 https://doi.org/10.1006/mthe.2002.0625	journal	July 2002
NMRPipe: A multidimensional spectral processing system based on UNIX pipes Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW. Journal of Biomolecular NMR, Vol. 6, Issue 3 https://doi.org/10.1007/bf00197809	journal	November 1995
Sparse multidimensional iterative lineshape-enhanced (SMILE) reconstruction of both non-uniformly sampled and conventional NMR data Ying, Jinfa; Delaglio, Frank; Torchia, Dennis A. Journal of Biomolecular NMR, Vol. 68, Issue 2 https://doi.org/10.1007/s10858-016-0072-7	journal	November 2016
1H1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins Bax, Ad; Clore, G. Marius; Gronenborn, Angela M. Journal of Magnetic Resonance (1969), Vol. 88, Issue 2 https://doi.org/10.1016/0022-2364(90)90202-K	journal	June 1990
Crystallographic refinement and structure of DNase I at 2 Å resolution Oefner, Christian; Suck, Dietrich Journal of Molecular Biology, Vol. 192, Issue 3 https://doi.org/10.1016/0022-2836(86)90280-9	journal	December 1986
DNase I-induced DNA conformation Lahm, Armin; Suck, Dietrich Journal of Molecular Biology, Vol. 222, Issue 3 https://doi.org/10.1016/0022-2836(91)90502-W	journal	December 1991
X-ray structure of the DNase I-d(GGTATACC)2 complex at 2·3Å resolution Weston, S. A.; Lahm, A.; Suck, D. Journal of Molecular Biology, Vol. 226, Issue 4 https://doi.org/10.1016/0022-2836(92)91064-V	journal	August 1992
Overproduction of the toxic protein, bovine pancreatic DNasel, in Escherichia coli using a tightly controlled T7-promoter-based vector Doherty, Aidan J.; Connolly, Bernard A.; Worrall, Andrew F. Gene, Vol. 136, Issue 1-2 https://doi.org/10.1016/0378-1119(93)90491-K	journal	December 1993
The chemical synthesis of a gene coding for bovine pancreatic DNase I and its cloning and expression in Escherichia coli. Worrall, A. F.; Connolly, B. A. Journal of Biological Chemistry, Vol. 265, Issue 35 https://doi.org/10.1016/S0021-9258(18)45822-X	journal	December 1990
Immunochip Analysis Identifies Multiple Susceptibility Loci for Systemic Sclerosis Mayes, Maureen D.; Bossini-Castillo, Lara; Gorlova, Olga The American Journal of Human Genetics, Vol. 94, Issue 1 https://doi.org/10.1016/j.ajhg.2013.12.002	journal	January 2014
Probing the catalytic mechanism of bovine pancreatic deoxyribonuclease I by chemical rescue Chen, Wei-Jung; Lai, Pei-Jun; Lai, Yu-Shen Biochemical and Biophysical Research Communications, Vol. 352, Issue 3 https://doi.org/10.1016/j.bbrc.2006.11.078	journal	January 2007
Caucasian-specific allele in non-synonymous single nucleotide polymorphisms of the gene encoding deoxyribonuclease I-like 3, potentially relevant to autoimmunity, produces an inactive enzyme Ueki, Misuzu; Takeshita, Haruo; Fujihara, Junko Clinica Chimica Acta, Vol. 407, Issue 1-2 https://doi.org/10.1016/j.cca.2009.06.022	journal	September 2009
Digestion of Chromatin in Apoptotic Cell Microparticles Prevents Autoimmunity Sisirak, Vanja; Sally, Benjamin; D’Agati, Vivette Cell, Vol. 166, Issue 1 https://doi.org/10.1016/j.cell.2016.05.034	journal	June 2016
Microparticles as antigenic targets of antibodies to DNA and nucleosomes in systemic lupus erythematosus Ullal, Anirudh J.; Reich, Charles F.; Clowse, Megan Journal of Autoimmunity, Vol. 36, Issue 3-4 https://doi.org/10.1016/j.jaut.2011.02.001	journal	May 2011
Enhancement of Pulmozyme activity in purulent sputum by combination with poly-aspartic acid or gelsolin Bucki, Robert; Cruz, Katrina; Pogoda, Katarzyna Journal of Cystic Fibrosis, Vol. 14, Issue 5 https://doi.org/10.1016/j.jcf.2015.02.001	journal	September 2015
Dnases in health and disease Keyel, Peter A. Developmental Biology, Vol. 429, Issue 1 https://doi.org/10.1016/j.ydbio.2017.06.028	journal	September 2017
The Rosetta All-Atom Energy Function for Macromolecular Modeling and Design Alford, Rebecca F.; Leaver-Fay, Andrew; Jeliazkov, Jeliazko R. Journal of Chemical Theory and Computation, Vol. 13, Issue 6 https://doi.org/10.1021/acs.jctc.7b00125	journal	May 2017
pH-Induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme Anderson, D. Eric; Becktel, Wayne J.; Dahlquist, Frederick W. Biochemistry, Vol. 29, Issue 9 https://doi.org/10.1021/bi00461a025	journal	March 1990
The Structure of Human DNase I Bound to Magnesium and Phosphate Ions Points to a Catalytic Mechanism Common to Members of the DNase I-like Superfamily Parsiegla, Goetz; Noguere, Christophe; Santell, Lydia Biochemistry, Vol. 51, Issue 51 https://doi.org/10.1021/bi300873f	journal	December 2012
NMR Structural Analysis of an Analog of an Intermediate Formed in the Rate-Determining Step of One Pathway in the Oxidative Folding of Bovine Pancreatic Ribonuclease A: Automated Analysis of ¹H, ¹³C, and ¹⁵N Resonance Assignments for Wild-Type and [C65S, C72S] Mutant Forms Shimotakahara, Sakurako; Ríos, Carlos B.; Laity, John H. Biochemistry, Vol. 36, Issue 23 https://doi.org/10.1021/bi963024k	journal	June 1997
Atomic structure of the actin: DNase I complex Kabsch, Wolfgang; Mannherz, Hans Georg; Suck, Dietrich Nature, Vol. 347, Issue 6288 https://doi.org/10.1038/347037a0	journal	September 1990
Loss-of-function variant in DNASE1L3 causes a familial form of systemic lupus erythematosus Al-Mayouf, Sulaiman M.; Sunker, Asma; Abdwani, Reem Nature Genetics, Vol. 43, Issue 12 https://doi.org/10.1038/ng.975	journal	October 2011
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
Fine-mapping and functional studies highlight potential causal variants for rheumatoid arthritis and type 1 diabetes Westra, Harm-Jan; Martínez-Bonet, Marta; Onengut-Gumuscu, Suna Nature Genetics, Vol. 50, Issue 10 https://doi.org/10.1038/s41588-018-0216-7	journal	September 2018
FerA is a Membrane-Associating Four-Helix Bundle Domain in the Ferlin Family of Membrane-Fusion Proteins Harsini, Faraz M.; Chebrolu, Sukanya; Fuson, Kerry L. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-29184-1	journal	July 2018
Systemic Lupus Erythematosus Tsokos, George C. New England Journal of Medicine, Vol. 365, Issue 22 https://doi.org/10.1056/NEJMra1100359	journal	December 2011
Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly Chereau, D.; Kerff, F.; Graceffa, P. Proceedings of the National Academy of Sciences, Vol. 102, Issue 46 https://doi.org/10.1073/pnas.0507021102	journal	November 2005
Consistent blind protein structure generation from NMR chemical shift data Shen, Y.; Lange, O.; Delaglio, F. Proceedings of the National Academy of Sciences, Vol. 105, Issue 12 https://doi.org/10.1073/pnas.0800256105	journal	March 2008
Connecting actin monomers by iso-peptide bond is a toxicity mechanism of the Vibrio cholerae MARTX toxin Kudryashov, Dmitri S.; Durer, Zeynep A. Oztug; Ytterberg, A. Jimmy Proceedings of the National Academy of Sciences, Vol. 105, Issue 47 https://doi.org/10.1073/pnas.0808082105	journal	November 2008
Engineering actin-resistant human DNase I for treatment of cystic fibrosis. Ulmer, J. S.; Herzka, A.; Toy, K. J. Proceedings of the National Academy of Sciences, Vol. 93, Issue 16 https://doi.org/10.1073/pnas.93.16.8225	journal	August 1996
Improved Potency of Hyperactive and Actin-resistant Human DNase I Variants for Treatment of Cystic Fibrosis and Systemic Lupus Erythematosus Pan, Clark Q.; Dodge, Tony H.; Baker, Dana L. Journal of Biological Chemistry, Vol. 273, Issue 29 https://doi.org/10.1074/jbc.273.29.18374	journal	July 1998
ATP Hydrolysis by RAD50 Protein Switches MRE11 Enzyme from Endonuclease to Exonuclease Majka, Jerzy; Alford, Brian; Ausio, Juan Journal of Biological Chemistry, Vol. 287, Issue 4 https://doi.org/10.1074/jbc.M111.307041	journal	January 2012
Autoantibody-mediated impairment of DNASE1L3 activity in sporadic systemic lupus erythematosus Hartl, Johannes; Serpas, Lee; Wang, Yueyang Journal of Experimental Medicine, Vol. 218, Issue 5 https://doi.org/10.1084/jem.20201138	journal	March 2021
Small-angle scattering studies of biological macromolecules in solution Svergun, Dmitri I.; Koch, Michel H. J. Reports on Progress in Physics, Vol. 66, Issue 10 https://doi.org/10.1088/0034-4885/66/10/R05	journal	September 2003
Bio3d: an R package for the comparative analysis of protein structures Grant, B. J.; Rodrigues, A. P. C.; ElSawy, K. M. Bioinformatics, Vol. 22, Issue 21 https://doi.org/10.1093/bioinformatics/btl461	journal	August 2006
H++: a server for estimating pKas and adding missing hydrogens to macromolecules Gordon, J. C.; Myers, J. B.; Folta, T. Nucleic Acids Research, Vol. 33, Issue Web Server https://doi.org/10.1093/nar/gki464	journal	July 2005
H++ 3.0: automating pK prediction and the preparation of biomolecular structures for atomistic molecular modeling and simulations Anandakrishnan, R.; Aguilar, B.; Onufriev, A. V. Nucleic Acids Research, Vol. 40, Issue W1 https://doi.org/10.1093/nar/gks375	journal	May 2012
FoXS, FoXSDock and MultiFoXS: Single-state and multi-state structural modeling of proteins and their complexes based on SAXS profiles Schneidman-Duhovny, Dina; Hammel, Michal; Tainer, John A. Nucleic Acids Research, Vol. 44, Issue W1 https://doi.org/10.1093/nar/gkw389	journal	May 2016
Mechanism and Catalytic Site Atlas (M-CSA): a database of enzyme reaction mechanisms and active sites Ribeiro, António J. M.; Holliday, Gemma L.; Furnham, Nicholas Nucleic Acids Research, Vol. 46, Issue D1 https://doi.org/10.1093/nar/gkx1012	journal	November 2017
Determination of the regularization parameter in indirect-transform methods using perceptual criteria Svergun, D. I. Journal of Applied Crystallography, Vol. 25, Issue 4, p. 495-503 https://doi.org/10.1107/S0021889892001663	journal	August 1992
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
ATSAS 3.0 : expanded functionality and new tools for small-angle scattering data analysis Manalastas-Cantos, Karen; Konarev, Petr V.; Hajizadeh, Nelly R. Journal of Applied Crystallography, Vol. 54, Issue 1 https://doi.org/10.1107/S1600576720013412	journal	February 2021
Biological functions of the disulfides in bovine pancreatic deoxyribonuclease Chen, W. -J. Protein Science, Vol. 13, Issue 4 https://doi.org/10.1110/ps.03438204	journal	April 2004
Murine serum nucleases - contrasting effects of plasmin and heparin on the activities of DNase1 and DNase1-like 3 (DNase1l3) Napirei, Markus; Ludwig, Sebastian; Mezrhab, Jamal FEBS Journal, Vol. 276, Issue 4 https://doi.org/10.1111/j.1742-4658.2008.06849.x	journal	January 2009
Host DNases prevent vascular occlusion by neutrophil extracellular traps Jiménez-Alcázar, Miguel; Rangaswamy, Chandini; Panda, Rachita Science, Vol. 358, Issue 6367 https://doi.org/10.1126/science.aam8897	journal	December 2017
Arg206Cys substitution in DNASE1L3 causes a defect in DNASE1L3 protein secretion that confers risk of systemic lupus erythematosus Coke, Latanya N.; Wen, Hongxiu; Comeau, Mary Annals of the Rheumatic Diseases, Vol. 80, Issue 6 https://doi.org/10.1136/annrheumdis-2020-218810	journal	January 2021
Improved treatment response to dornase alfa in cystic fibrosis patients using controlled inhalation Bakker, E. M.; Volpi, S.; Salonini, E. European Respiratory Journal, Vol. 38, Issue 6 https://doi.org/10.1183/09031936.00006211	journal	July 2011
Quantitative approaches to monitor protein-nucleic acid interactions using fluorescent probes Pagano, J. M.; Clingman, C. C.; Ryder, S. P. RNA, Vol. 17, Issue 1 https://doi.org/10.1261/rna.2428111	journal	November 2010
Two N-Linked Glycosylation Sites (Asn18 and Asn106) Are Both Required for Full Enzymatic Activity, Thermal Stability, and Resistance to Proteolysis in Mammalian Deoxyribonuclease I Fujihara, Junko; Yasuda, Toshihiro; Kunito, Takashi Bioscience, Biotechnology, and Biochemistry, Vol. 72, Issue 12 https://doi.org/10.1271/bbb.80376	journal	December 2008
pH-Induced Denaturation of Proteins: A Single Salt Bridge Contributes 3-5 kcal/mol to the Free Energy of Folding of T4 Lysozyme Anderson, D. Eric; Becktel, Wayne J.; Dahlquist, Frederick W. Biological Magnetic Resonance Bank https://doi.org/10.13018/bmr887	dataset	January 1999
How Cations Can Assist DNase I in DNA Binding and Hydrolysis Guéroult, Marc; Picot, Daniel; Abi-Ghanem, Joséphine PLoS Computational Biology, Vol. 6, Issue 11 https://doi.org/10.1371/journal.pcbi.1001000	journal	November 2010
The Ability to Enhance the Solubility of Its Fusion Partners Is an Intrinsic Property of Maltose-Binding Protein but Their Folding Is Either Spontaneous or Chaperone-Mediated Raran-Kurussi, Sreejith; Waugh, David S. PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0049589	journal	November 2012
Differential Dynamic Engagement within 24 SH3 Domain: Peptide Complexes Revealed by Co-Linear Chemical Shift Perturbation Analysis Stollar, Elliott J.; Lin, Hong; Davidson, Alan R. PLoS ONE, Vol. 7, Issue 12 https://doi.org/10.1371/journal.pone.0051282	journal	December 2012
Increased Concentrations of Antibody-Bound Circulatory Cell-Free DNA in Rheumatoid Arthritis Zhong, Xiao-Yan; von Mühlenen, Ines; Li, Ying Clinical Chemistry, Vol. 53, Issue 9 https://doi.org/10.1373/clinchem.2006.084509	journal	September 2007
Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion Shi, Guilan; Abbott, Kennady N.; Wu, Wenbo Frontiers in Immunology, Vol. 8 https://doi.org/10.3389/fimmu.2017.00522	journal	May 2017
Self-DNA at the Epicenter of SLE: Immunogenic Forms, Regulation, and Effects Soni, Chetna; Reizis, Boris Frontiers in Immunology, Vol. 10 https://doi.org/10.3389/fimmu.2019.01601	journal	July 2019
Actin-Resistant DNase1L2 as a Potential Therapeutics for CF Lung Disease Delfino, Danila; Mori, Giulia; Rivetti, Claudio Biomolecules, Vol. 11, Issue 3 https://doi.org/10.3390/biom11030410	journal	March 2021
Dnase1 Family in Autoimmunity Engavale, Minal; McCord, Jon; Mapp, Britney Encyclopedia, Vol. 1, Issue 3 https://doi.org/10.3390/encyclopedia1030044	journal	July 2021
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated Raran-Kurussi, Sreejith; Waugh, David S. Plos One, Vol. 7, Issue 11, e49589 https://doi.org/10.3410/f.717968797.793468506	journal	November 2012
Association of the DNASE1L3 rs35677470 polymorphism with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis: Structural biological insights Zervou, Maria; Andreou, Athena; Matalliotakis, Michail Molecular Medicine Reports, Vol. 22, Issue 6 https://doi.org/10.3892/mmr.2020.11547	journal	September 2020

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Structural features of Dnase1L3 responsible for serum antigen clearance

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