Metabolic flux modeling of Gluconobacter oxydans enables improved production of bioleaching organic acids

Aston, John E.; Thompson, Vicki S.; Fujita, Yoshiko; Reed, David W.

doi:10.1016/j.procbio.2022.10.027

Metabolic flux modeling of Gluconobacter oxydans enables improved production of bioleaching organic acids

Journal Article · Wed Oct 26 00:00:00 EDT 2022 · Process Biochemistry

DOI:https://doi.org/10.1016/j.procbio.2022.10.027· OSTI ID:1899149

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Idaho National Lab. (INL), Idaho Falls, ID (United States)

Bioleaching with organic acids is an option for recovery of rare earth elements (REE) and or other valuable metals from consumer or industrial wastes. Biohydrometallurgy can play an economically and environmentally sustainable role in REE recycling, particularly if agriculture or food wastes are used as the carbon substrates for production of the bioleaching agents. A challenge for organic acid based REE bioleaching is the incomplete conversion of the carbon substrate to an optimal organic acid mixture with high REE extraction capability. To help meet this challenge a metabolic flux model was developed for Gluconobacter oxydans to identify options for improving sugar conversion to chelating organic acids. Here, model predictions were consistent with laboratory data for the microbial consumption of glucose and production of gluconic acid and suggested that providing growth conditions with relatively high carbon to nitrogen molar ratios could result in production of lower pH biolixiviant and thus improve REE bioleaching yields.

View Accepted Manuscript (DOE)

Research Organization:: Idaho National Laboratory (INL), Idaho Falls, ID (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office

Grant/Contract Number:: AC07-05ID14517

OSTI ID:: 1899149

Report Number(s):: INL/JOU-20-55818-Rev002

Journal Information:: Process Biochemistry, Journal Name: Process Biochemistry Journal Issue: Part 2 Vol. 122; ISSN 1359-5113

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
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metabolic modeling
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Metabolic flux modeling of Gluconobacter oxydans enables improved production of bioleaching organic acids

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