Gene editing of Gluconobacter oxydans for improved xylose metabolism and bioleaching
Conference
·
OSTI ID:1546711
- Idaho National Laboratory
Rare earth elements can be recycled using bioleaching methods that employ agricultural waste as the carbon source. One of the most abundant sources of this waste is corn stover, which contains both glucose and xylose sugars. The chosen bacteria, Gluconobacter oxydans (G. ox), has low efficiency for metabolizing xylose, which can affect the concentration of organic acids produced. This research focused on the introduction of a plasmid for xylose metabolism into the G. ox genome. A triparental mating method was used. Success of the conjugation was verified with a plasmid prep and restriction digest. The restriction digest suggested some unexpected damage to the plasmid DNA, which will require some backtracking to remedy. In the future, the cells with altered genetics will be used in experimentation with the production of corn stover lixiviant.
- Research Organization:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- DOE Contract Number:
- AC07-05ID14517
- OSTI ID:
- 1546711
- Report Number(s):
- INL/EXP-19-55116-Rev000
- Country of Publication:
- United States
- Language:
- English
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