AMX – the highly automated macromolecular crystallography (17-ID-1) beamline at the NSLS-II

Schneider, Dieter K.; Soares, Alexei S.; Lazo, Edwin O.; Kreitler, Dale F.; Qian, Kun; Fuchs, Martin R.; Bhogadi, Dileep K.; Antonelli, Steve; Myers, Stuart S.; Martins, Bruno S.; Skinner, John M.; Aishima, Jun; Bernstein, Herbert J.; Langdon, Thomas; Lara, John; Petkus, Robert; Cowan, Matt; Flaks, Leonid; Smith, Thomas; Shea-McCarthy, Grace; Idir, Mourad; Huang, Lei; Chubar, Oleg; Sweet, Robert M.; Berman, Lonny E.; McSweeney, Sean; Jakoncic, Jean

doi:10.1107/S1600577522009377

Title: AMX – the highly automated macromolecular crystallography (17-ID-1) beamline at the NSLS-II

Journal Article · Fri Oct 21 00:00:00 EDT 2022 · Journal of Synchrotron Radiation (Online)

DOI:https://doi.org/10.1107/S1600577522009377· OSTI ID:1894176

;

; Kreitler, Dale F.; Qian, Kun;

; Bhogadi, Dileep K.;

; Myers, Stuart S.; Martins, Bruno S.; Skinner, John M.;

;

; Langdon, Thomas; Lara, John; Petkus, Robert; Cowan, Matt; Flaks, Leonid; Smith, Thomas; Shea-McCarthy, Grace more »

The highly automated macromolecular crystallography beamline AMX/17-ID-1 is an undulator-based high-intensity (>5 × 10 ¹² photons s ⁻¹ ), micro-focus (7 µm × 5 µm), low-divergence (1 mrad × 0.35 mrad) energy-tunable (5–18 keV) beamline at the NSLS-II, Brookhaven National Laboratory, Upton, NY, USA. It is one of the three life science beamlines constructed by the NIH under the ABBIX project and it shares sector 17-ID with the FMX beamline, the frontier micro-focus macromolecular crystallography beamline. AMX saw first light in March 2016 and started general user operation in February 2017. At AMX, emphasis has been placed on high throughput, high capacity, and automation to enable data collection from the most challenging projects using an intense micro-focus beam. Here, the current state and capabilities of the beamline are reported, and the different macromolecular crystallography experiments that are routinely performed at AMX/17-ID-1 as well as some plans for the near future are presented.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0012704

OSTI ID:: 1894176

Alternate ID(s):: OSTI ID: 1922805

Report Number(s):: BNL-223941-2023-JAAM; JSYRES; PII: S1600577522009377

Journal Information:: Journal of Synchrotron Radiation (Online), Journal Name: Journal of Synchrotron Radiation (Online) Vol. 29 Journal Issue: 6; ISSN 1600-5775

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: Denmark

Language:: English

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36 MATERIALS SCIENCE
macromolecular crystallography
automation
beamline
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high throughput
micro-beam
real-time feedback

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