Comparative chemical genomic profiling across plant-based hydrolysate toxins reveals widespread antagonism in fitness contributions

Vanacloig-Pedros, Elena; Fisher, Kaitlin J.; Liu, Lisa; Debrauske, Derek J.; Young, Megan K. M.; Place, Michael; Hittinger, Chris Todd; Sato, Trey K.; Gasch, Audrey P.

doi:10.1093/femsyr/foac036

Title: Comparative chemical genomic profiling across plant-based hydrolysate toxins reveals widespread antagonism in fitness contributions

Journal Article · Tue Jul 26 00:00:00 EDT 2022 · FEMS Yeast Research

DOI:https://doi.org/10.1093/femsyr/foac036· OSTI ID:1889283

; Fisher, Kaitlin J.; Liu, Lisa; Debrauske, Derek J.; Young, Megan K. M.; Place, Michael;

; Sato, Trey K.; Gasch, Audrey P.

Abstract The budding yeast Saccharomyces cerevisiae has been used extensively in fermentative industrial processes, including biofuel production from sustainable plant-based hydrolysates. Myriad toxins and stressors found in hydrolysates inhibit microbial metabolism and product formation. Overcoming these stresses requires mitigation strategies that include strain engineering. To identify shared and divergent mechanisms of toxicity and to implicate gene targets for genetic engineering, we used a chemical genomic approach to study fitness effects across a library of S. cerevisiae deletion mutants cultured anaerobically in dozens of individual compounds found in different types of hydrolysates. Relationships in chemical genomic profiles identified classes of toxins that provoked similar cellular responses, spanning inhibitor relationships that were not expected from chemical classification. Our results also revealed widespread antagonistic effects across inhibitors, such that the same gene deletions were beneficial for surviving some toxins but detrimental for others. This work presents a rich dataset relating gene function to chemical compounds, which both expands our understanding of plant-based hydrolysates and provides a useful resource to identify engineering targets.

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Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0018409; FC02-07ER64494

OSTI ID:: 1889283

Alternate ID(s):: OSTI ID: 1893933

Journal Information:: FEMS Yeast Research, Journal Name: FEMS Yeast Research Vol. 22 Journal Issue: 1; ISSN 1567-1364

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (86)

A Protein Conjugation System in Yeast with Homology to Biosynthetic Enzyme Reaction of Prokaryotes Furukawa, Kazuaki; Mizushima, Noboru; Noda, Takeshi Journal of Biological Chemistry, Vol. 275, Issue 11 https://doi.org/10.1074/jbc.275.11.7462	journal	March 2000
UpSetR: an R package for the visualization of intersecting sets and their properties Conway, Jake R.; Lex, Alexander; Gehlenborg, Nils Bioinformatics, Vol. 33, Issue 18 https://doi.org/10.1093/bioinformatics/btx364	journal	June 2017
Vacuolar and Plasma Membrane Proton Pumps Collaborate to Achieve Cytosolic pH Homeostasis in Yeast Martínez-Muñoz, Gloria A.; Kane, Patricia Journal of Biological Chemistry, Vol. 283, Issue 29 https://doi.org/10.1074/jbc.M710470200	journal	July 2008
Nonenzymatic Sugar Production from Biomass Using Biomass-Derived -Valerolactone Luterbacher, J. S.; Rand, J. M.; Alonso, D. M. Science, Vol. 343, Issue 6168 https://doi.org/10.1126/science.1246748	journal	January 2014
Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae Almeida, Joao; Modig, Tobias; Petersson, Anneli Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349 https://doi.org/10.1002/jctb.1676	journal	January 2007
Bugs, drugs and chemical genomics Roemer, Terry; Davies, Julian; Giaever, Guri Nature Chemical Biology, Vol. 8, Issue 1 https://doi.org/10.1038/nchembio.744	journal	December 2011
Bioconversion of lignocellulose: inhibitors and detoxification Jönsson, Leif J.; Alriksson, Björn; Nilvebrant, Nils-Olof Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-16	journal	January 2013
Dissolution of Cellose with Ionic Liquids Swatloski, Richard P.; Spear, Scott K.; Holbrey, John D. Journal of the American Chemical Society, Vol. 124, Issue 18, p. 4974-4975 https://doi.org/10.1021/ja025790m	journal	May 2002
Molecular Origins of Complex Heritability in Natural Genotype-to-Phenotype Relationships Jakobson, Christopher M.; Jarosz, Daniel F. Cell Systems, Vol. 8, Issue 5 https://doi.org/10.1016/j.cels.2019.04.002	journal	May 2019
Integrated conversion of hemicellulose and cellulose from lignocellulosic biomass Alonso, David Martin; Wettstein, Stephanie G.; Mellmer, Max A. Energy Environ. Sci., Vol. 6, Issue 1 https://doi.org/10.1039/C2EE23617F	journal	January 2013
Pretreatment of lignocellulose: Formation of inhibitory by-products and strategies for minimizing their effects Jönsson, Leif J.; Martín, Carlos Bioresource Technology, Vol. 199 https://doi.org/10.1016/j.biortech.2015.10.009	journal	January 2016
Multi-Faceted Systems Biology Approaches Present a Cellular Landscape of Phenolic Compound Inhibition in Saccharomyces cerevisiae Fletcher, Eugene; Baetz, Kristin Frontiers in Bioengineering and Biotechnology, Vol. 8 https://doi.org/10.3389/fbioe.2020.539902	journal	October 2020
Yeast chemogenomic screen identifies distinct metabolic pathways required to tolerate exposure to phenolic fermentation inhibitors ferulic acid, 4-hydroxybenzoic acid and coniferyl aldehyde Fletcher, Eugene; Gao, Kai; Mercurio, Kevin Metabolic Engineering, Vol. 52 https://doi.org/10.1016/j.ymben.2018.11.010	journal	March 2019
Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass Klinke, H. B.; Thomsen, A. B.; Ahring, B. K. Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26 https://doi.org/10.1007/s00253-004-1642-2	journal	August 2004
The GET Complex Mediates Insertion of Tail-Anchored Proteins into the ER Membrane Schuldiner, Maya; Metz, Jutta; Schmid, Volker Cell, Vol. 134, Issue 4 https://doi.org/10.1016/j.cell.2008.06.025	journal	August 2008
Plant Maturity Effects on the Physicochemical Properties and Dilute Acid Hydrolysis of Switchgrass ( Panicum virgatum , L.) Hemicelluloses Bunnell, Kris; Rich, Ashley; Luckett, Curtis ACS Sustainable Chemistry & Engineering, Vol. 1, Issue 6 https://doi.org/10.1021/sc4000175	journal	April 2013
The Yeast Deletion Collection: A Decade of Functional Genomics Giaever, Guri; Nislow, Corey Genetics, Vol. 197, Issue 2 https://doi.org/10.1534/genetics.114.161620	journal	June 2014
Multiomic Fermentation Using Chemically Defined Synthetic Hydrolyzates Revealed Multiple Effects of Lignocellulose-Derived Inhibitors on Cell Physiology and Xylose Utilization in Zymomonas mobilis Zhang, Yaoping; Vera, Jessica M.; Xie, Dan Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.02596	journal	November 2019
Mechanism of imidazolium ionic liquids toxicity in Saccharomyces cerevisiae and rational engineering of a tolerant, xylose-fermenting strain Dickinson, Quinn; Bottoms, Scott; Hinchman, Li Microbial Cell Factories, Vol. 15, Issue 1 https://doi.org/10.1186/s12934-016-0417-7	journal	January 2016
Physico-Chemical Conversion of Lignocellulose: Inhibitor Effects and Detoxification Strategies: A Mini Review Kim, Daehwan Molecules, Vol. 23, Issue 2 https://doi.org/10.3390/molecules23020309	journal	February 2018
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose Socha, A. M.; Parthasarathi, R.; Shi, J. Proceedings of the National Academy of Sciences, Vol. 111, Issue 35 https://doi.org/10.1073/pnas.1405685111	journal	August 2014
Combining functional genomics and chemical biology to identify targets of bioactive compounds Ho, Cheuk Hei; Piotrowski, Jeff; Dixon, Scott J. Current Opinion in Chemical Biology, Vol. 15, Issue 1 https://doi.org/10.1016/j.cbpa.2010.10.023	journal	February 2011
Barcode technology in yeast: application to pharmacogenomics: Pharmacogenomics with yeast Delneri, Daniela FEMS Yeast Research, Vol. 10, Issue 8 https://doi.org/10.1111/j.1567-1364.2010.00676.x	journal	November 2010
Dissecting a complex chemical stress: chemogenomic profiling of plant hydrolysates Skerker, Jeffrey M.; Leon, Dacia; Price, Morgan N. Molecular Systems Biology, Vol. 9, Issue 1 https://doi.org/10.1038/msb.2013.30	journal	January 2013
Genome-Wide Analysis Reveals the Vacuolar pH-Stat of Saccharomyces cerevisiae Brett, Christopher L.; Kallay, Laura; Hua, Zhaolin PLoS ONE, Vol. 6, Issue 3 https://doi.org/10.1371/journal.pone.0017619	journal	March 2011
A higher spirit: avoiding yeast suicide during alcoholic fermentation Carmona-Gutierrez, D.; Sommer, C.; Andryushkova, A. Cell Death & Differentiation, Vol. 19, Issue 6 https://doi.org/10.1038/cdd.2012.31	journal	May 2012
Mapping the Cellular Response to Small Molecules Using Chemogenomic Fitness Signatures Lee, A. Y.; St. Onge, R. P.; Proctor, M. J. Science, Vol. 344, Issue 6180 https://doi.org/10.1126/science.1250217	journal	April 2014
A Novel Mechanism of Ion Homeostasis and Salt Tolerance in Yeast: the Hal4 and Hal5 Protein Kinases Modulate the Trk1-Trk2 Potassium Transporter Mulet, Jose M.; Leube, Martin P.; Kron, Stephen J. Molecular and Cellular Biology, Vol. 19, Issue 5 https://doi.org/10.1128/MCB.19.5.3328	journal	May 1999
Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae Ding, Junmei; Huang, Xiaowei; Zhang, Lemin Applied Microbiology and Biotechnology, Vol. 85, Issue 2 https://doi.org/10.1007/s00253-009-2223-1	journal	September 2009
Functional annotation of chemical libraries across diverse biological processes Piotrowski, Jeff S.; Li, Sheena C.; Deshpande, Raamesh Nature Chemical Biology, Vol. 13, Issue 9 https://doi.org/10.1038/nchembio.2436	journal	July 2017
Incorporating comparative genomics into the design–test–learn cycle of microbial strain engineering Sardi, Maria; Gasch, Audrey P. FEMS Yeast Research, Vol. 17, Issue 5 https://doi.org/10.1093/femsyr/fox042	journal	June 2017
Anion effects on the cytotoxicity of ionic liquids Stolte, Stefan; Arning, J?rgen; Bottin-Weber, Ulrike Green Chemistry, Vol. 8, Issue 7 https://doi.org/10.1039/b602161a	journal	January 2006
Multiple activities of Arl1 GTPase in the trans-Golgi network Yu, Chia-Jung; Lee, Fang-Jen S. Journal of Cell Science https://doi.org/10.1242/jcs.201319	journal	January 2017
Data mining of Saccharomyces cerevisiae mutants engineered for increased tolerance towards inhibitors in lignocellulosic hydrolysates Cámara, Elena; Olsson, Lisbeth; Zrimec, Jan Biotechnology Advances, Vol. 57 https://doi.org/10.1016/j.biotechadv.2022.107947	journal	July 2022
Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis Winzeler, E. A. Science, Vol. 285, Issue 5429 https://doi.org/10.1126/science.285.5429.901	journal	August 1999
Molecular and physiological basis of Saccharomyces cerevisiae tolerance to adverse lignocellulose-based process conditions Cunha, Joana T.; Romaní, Aloia; Costa, Carlos E. Applied Microbiology and Biotechnology, Vol. 103, Issue 1 https://doi.org/10.1007/s00253-018-9478-3	journal	November 2018
Molecular mechanisms of yeast tolerance and in situ detoxification of lignocellulose hydrolysates Liu, Z. Lewis Applied Microbiology and Biotechnology, Vol. 90, Issue 3 https://doi.org/10.1007/s00253-011-3167-9	journal	March 2011
Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate Ong, Rebecca Garlock; Higbee, Alan; Bottoms, Scott Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0657-0	journal	November 2016
Impact of ionic liquid pretreated plant biomass on Saccharomyces cerevisiae growth and biofuel production Ouellet, Mario; Datta, Supratim; Dibble, Dean C. Green Chemistry, Vol. 13, Issue 10 https://doi.org/10.1039/c1gc15327g	journal	January 2011
Investigation of Bar-seq as a method to study population dynamics of Saccharomyces cerevisiae deletion library during bioreactor cultivation Wehrs, Maren; Thompson, Mitchell G.; Banerjee, Deepanwita Microbial Cell Factories, Vol. 19, Issue 1 https://doi.org/10.1186/s12934-020-01423-z	journal	August 2020
Alkali Metal Cation Transport and Homeostasis in Yeasts Ariño, Joaquín; Ramos, José; Sychrová, Hana Microbiology and Molecular Biology Reviews, Vol. 74, Issue 1 https://doi.org/10.1128/MMBR.00042-09	journal	March 2010
Mechanisms of action of ionic liquids on living cells: the state of the art Kumari, Pallavi; Pillai, Visakh V. S.; Benedetto, Antonio Biophysical Reviews, Vol. 12, Issue 5 https://doi.org/10.1007/s12551-020-00754-w	journal	September 2020
Substrate and Product Inhibition on Yeast Performance in Ethanol Fermentation Zhang, Qi; Wu, Deyi; Lin, Yan Energy & Fuels, Vol. 29, Issue 2 https://doi.org/10.1021/ef502349v	journal	February 2015
Genetic background effects in quantitative genetics: gene-by-system interactions Sardi, Maria; Gasch, Audrey P. Current Genetics, Vol. 64, Issue 6 https://doi.org/10.1007/s00294-018-0835-7	journal	April 2018
A new efficient gene disruption cassette for repeated use in budding yeast Guldener, U. Nucleic Acids Research, Vol. 24, Issue 13 https://doi.org/10.1093/nar/24.13.2519	journal	July 1996
Water-soluble phenolic compounds produced from extractive ammonia pretreatment exerted binary inhibitory effects on yeast fermentation using synthetic hydrolysate Xue, Saisi; Jones, A. Daniel; Sousa, Leonardo PLOS ONE, Vol. 13, Issue 3 https://doi.org/10.1371/journal.pone.0194012	journal	March 2018
Future Microbial Applications for Bioenergy Production: A Perspective Kumar, Ravinder; Kumar, Pradeep Frontiers in Microbiology, Vol. 8 https://doi.org/10.3389/fmicb.2017.00450	journal	March 2017
Yeast protein kinase Ptk2 localizes at the plasma membrane and phosphorylates in vitro the C-terminal peptide of the H+-ATPase Eraso, Pilar; Mazón, María J.; Portillo, Francisco Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 2 https://doi.org/10.1016/j.bbamem.2006.01.010	journal	February 2006
Engineering alcohol tolerance in yeast Lam, Felix H.; Ghaderi, Adel; Fink, Gerald R. Science, Vol. 346, Issue 6205 https://doi.org/10.1126/science.1257859	journal	October 2014
Natural Variation in the Multidrug Efflux Pump SGE1 Underlies Ionic Liquid Tolerance in Yeast Higgins, Douglas A.; Young, Megan K. M.; Tremaine, Mary Genetics, Vol. 210, Issue 1 https://doi.org/10.1534/genetics.118.301161	journal	July 2018
Chemical Genetics: Budding Yeast as a Platform for Drug Discovery and Mapping of Genetic Pathways Enserink, Jorrit M. Molecules, Vol. 17, Issue 8 https://doi.org/10.3390/molecules17089258	journal	August 2012
edgeR: a Bioconductor package for differential expression analysis of digital gene expression data Robinson, M. D.; McCarthy, D. J.; Smyth, G. K. Bioinformatics, Vol. 26, Issue 1 https://doi.org/10.1093/bioinformatics/btp616	journal	November 2009
Role of Alcohols in Growth, Lipid Composition, and Membrane Fluidity of Yeasts, Bacteria, and Archaea Huffer, Sarah; Clark, Melinda E.; Ning, Jonathan C. Applied and Environmental Microbiology, Vol. 77, Issue 18 https://doi.org/10.1128/AEM.00694-11	journal	July 2011
Pretreatment of Lignocellulosic Biomass with Ionic Liquids and Ionic Liquid-Based Solvent Systems Hou, Qidong; Ju, Meiting; Li, Weizun Molecules, Vol. 22, Issue 3 https://doi.org/10.3390/molecules22030490	journal	March 2017
The many faces of pleiotropy Paaby, Annalise B.; Rockman, Matthew V. Trends in Genetics, Vol. 29, Issue 2 https://doi.org/10.1016/j.tig.2012.10.010	journal	February 2013
Effect of ethanol on fluxes of water and protons across the plasma membrane of Saccharomyces cerevisiae: Water and H+ fluxes across yeast plasma membrane Madeira, Ana; Leitão, Luís; Soveral, Graça FEMS Yeast Research, Vol. 10, Issue 3 https://doi.org/10.1111/j.1567-1364.2010.00607.x	journal	April 2010
Membrane interactions of ionic liquids: Possible determinants for biological activity and toxicity Gal, Noga; Malferarri, Danilo; Kolusheva, Sofiya Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1818, Issue 12 https://doi.org/10.1016/j.bbamem.2012.07.025	journal	December 2012
Controlling gene expression in yeast by inducible site-specific recombination Cheng, T. -H. Nucleic Acids Research, Vol. 28, Issue 24 https://doi.org/10.1093/nar/28.24.e108	journal	December 2000
Signaling Events of the Rim101 Pathway Occur at the Plasma Membrane in a Ubiquitination-Dependent Manner Obara, Keisuke; Kihara, Akio Molecular and Cellular Biology, Vol. 34, Issue 18 https://doi.org/10.1128/MCB.00408-14	journal	September 2014
The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter Forment, Javier; Mulet, Jose M.; Vicente, Oscar Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1565, Issue 1 https://doi.org/10.1016/S0005-2736(02)00503-5	journal	September 2002
The impacts of pretreatment on the fermentability of pretreated lignocellulosic biomass: a comparative evaluation between ammonia fiber expansion and dilute acid pretreatment Lau, Ming W.; Gunawan, Christa; Dale, Bruce E. Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-30	journal	January 2009
Yeast Systems Biology: Model Organism and Cell Factory Nielsen, Jens Biotechnology Journal, Vol. 14, Issue 9 https://doi.org/10.1002/biot.201800421	journal	May 2019
Genome-wide screening of Saccharomyces cerevisiae genes required to foster tolerance towards industrial wheat straw hydrolysates Pereira, Francisco B.; Teixeira, Miguel C.; Mira, Nuno P. Journal of Industrial Microbiology and Biotechnology, Vol. 41, Issue 12 https://doi.org/10.1007/s10295-014-1519-z	journal	December 2014
Correlating Lipid Membrane Permeabilities of Imidazolium Ionic Liquids with their Cytotoxicities on Yeast, Bacterial, and Mammalian Cells Biomolecules, Vol. 9, Issue 6 https://doi.org/10.3390/biom9060251	journal	June 2019
Utilizing yeast chemogenomic profiles for the prediction of pharmacogenomic associations in humans Silberberg, Yael; Kupiec, Martin; Sharan, Roded Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep23703	journal	March 2016
Java Treeview--extensible visualization of microarray data Saldanha, A. J. Bioinformatics, Vol. 20, Issue 17 https://doi.org/10.1093/bioinformatics/bth349	journal	June 2004
Recent Trends in the Pretreatment of Lignocellulosic Biomass for Value-Added Products Baruah, Julie; Nath, Bikash Kar; Sharma, Ritika Frontiers in Energy Research, Vol. 6 https://doi.org/10.3389/fenrg.2018.00141	journal	December 2018
Yeast ARL1 encodes a regulator of K+ influx Munson, Amanda M.; Haydon, Devon H.; Love, Sherie L. Journal of Cell Science, Vol. 117, Issue 11 https://doi.org/10.1242/jcs.01050	journal	May 2004
Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors Piotrowski, Jeff S.; Zhang, Yaoping; Bates, Donna M. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00090	journal	March 2014
Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments Chundawat, Shishir P. S.; Vismeh, Ramin; Sharma, Lekh N. Bioresource Technology, Vol. 101, Issue 21 https://doi.org/10.1016/j.biortech.2010.06.027	journal	November 2010
mclust 5: Clustering, Classification and Density Estimation Using Gaussian Finite Mixture Models Scrucca, Luca; Fop, Michael; Murphy, T. ,Brendan The R Journal, Vol. 8, Issue 1 https://doi.org/10.32614/RJ-2016-021	journal	January 2016
Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition Palmqvist, Eva; Hahn-Hägerdal, Bärbel Bioresource Technology, Vol. 74, Issue 1, p. 25-33 https://doi.org/10.1016/S0960-8524(99)00161-3	journal	August 2000
Critical Roles of the Pentose Phosphate Pathway and GLN3 in Isobutanol-Specific Tolerance in Yeast Kuroda, Kouichi; Hammer, Sarah K.; Watanabe, Yukio Cell Systems, Vol. 9, Issue 6 https://doi.org/10.1016/j.cels.2019.10.006	journal	December 2019
Regulation of Yeast H+-ATPase by Protein Kinases Belonging to a Family Dedicated to Activation of Plasma Membrane Transporters Goossens, A.; de la Fuente, N.; Forment, J. Molecular and Cellular Biology, Vol. 20, Issue 20 https://doi.org/10.1128/MCB.20.20.7654-7661.2000	journal	October 2000
Regulation of Cation Balance in Saccharomyces cerevisiae Cyert, Martha S.; Philpott, Caroline C. Genetics, Vol. 193, Issue 3 https://doi.org/10.1534/genetics.112.147207	journal	March 2013
The Identification of Genetic Determinants of Methanol Tolerance in Yeast Suggests Differences in Methanol and Ethanol Toxicity Mechanisms and Candidates for Improved Methanol Tolerance Engineering Mota, Marta N.; Martins, Luís C.; Sá-Correia, Isabel Journal of Fungi, Vol. 7, Issue 2 https://doi.org/10.3390/jof7020090	journal	January 2021
Functional profiling of the Saccharomyces cerevisiae genome Giaever, Guri; Chu, Angela M.; Ni, Li Nature, Vol. 418, Issue 6896 https://doi.org/10.1038/nature00935	journal	July 2002
Membrane Targeting: Getting Arl to the Golgi Graham, Todd R. Current Biology, Vol. 14, Issue 12 https://doi.org/10.1016/j.cub.2004.06.017	journal	June 2004
The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes Hillenmeyer, M. E.; Fung, E.; Wildenhain, J. Science, Vol. 320, Issue 5874 https://doi.org/10.1126/science.1150021	journal	April 2008
Aromatic inhibitors derived from ammonia-pretreated lignocellulose hinder bacterial ethanologenesis by activating regulatory circuits controlling inhibitor efflux and detoxification Keating, David H.; Zhang, Yaoping; Ong, Irene M. Frontiers in Microbiology, Vol. 5 https://doi.org/10.3389/fmicb.2014.00402	journal	August 2014
The ER membrane insertase Get1/2 is required for efficient mitophagy in yeast Onishi, Mashun; Nagumo, Sachiyo; Iwashita, Shohei Biochemical and Biophysical Research Communications, Vol. 503, Issue 1 https://doi.org/10.1016/j.bbrc.2018.04.114	journal	September 2018
Effects of aromatic compounds on the production of bacterial nanocellulose by Gluconacetobacter xylinus Zhang, Shuo; Winestrand, Sandra; Guo, Xiang Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/1475-2859-13-62	journal	January 2014
Vacuolar H ⁺ -ATPase Protects Saccharomyces cerevisiae Cells against Ethanol-Induced Oxidative and Cell Wall Stresses Charoenbhakdi, Sirikarn; Dokpikul, Thanittra; Burphan, Thanawat Applied and Environmental Microbiology, Vol. 82, Issue 10 https://doi.org/10.1128/AEM.00376-16	journal	May 2016
Recent advancements in fungal-derived fuel and chemical production and commercialization Ekas, Holly; Deaner, Matthew; Alper, Hal S. Current Opinion in Biotechnology, Vol. 57 https://doi.org/10.1016/j.copbio.2018.08.014	journal	June 2019
The Genomic Landscape and Evolutionary Resolution of Antagonistic Pleiotropy in Yeast Qian, Wenfeng; Ma, Di; Xiao, Che Cell Reports, Vol. 2, Issue 5 https://doi.org/10.1016/j.celrep.2012.09.017	journal	November 2012
Chemical genomic guided engineering of gamma-valerolactone tolerant yeast Bottoms, Scott; Dickinson, Quinn; McGee, Mick Microbial Cell Factories, Vol. 17, Issue 1 https://doi.org/10.1186/s12934-017-0848-9	journal	January 2018

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Title: Comparative chemical genomic profiling across plant-based hydrolysate toxins reveals widespread antagonism in fitness contributions

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