Sugar loading is not required for phloem sap flow in maize plants

Babst, Benjamin A.; Braun, David M.; Karve, Abhijit A.; Frank Baker, R.; Tran, Thu M.; Kenny, Douglas J.; Rohlhill, Julia; Knoblauch, Jan; Knoblauch, Michael; Lohaus, Gertrud; Tappero, Ryan; Scherzer, Sönke; Hedrich, Rainer; Jensen, Kaare H.

doi:10.1038/s41477-022-01098-x

Sugar loading is not required for phloem sap flow in maize plants

Journal Article · Mon Feb 21 00:00:00 EST 2022 · Nature Plants (Online)

DOI:https://doi.org/10.1038/s41477-022-01098-x· OSTI ID:1887533

^[1]; ^[2]; Karve, Abhijit A. ^[1]; Frank Baker, R. ^[2]; Tran, Thu M. ^[2]; ^[1]; ^[1]; ^[3]; ^[3]; Lohaus, Gertrud ^[4]; Tappero, Ryan ^[1]; ^[5]; ^[5]; ^[6]

Brookhaven National Lab. (BNL), Upton, NY (United States)
University of Missouri, Columbia, MO (United States)
Washington State University, Pullman, WA (United States)
University of Wuppertal (Germany)
University of Wurzburg (Germany)
Technical University of Denmark, Lyngby (Denmark)

We report phloem transport of photoassimilates from leaves to non-photosynthetic organs, such as the root and shoot apices and reproductive organs, is crucial to plant growth and yield. For nearly 90 years, evidence has been generally consistent with the theory of a pressure-flow mechanism of phloem transport. Central to this hypothesis is the loading of osmolytes, principally sugars, into the phloem to generate the osmotic pressure that propels bulk flow. Here we used genetic and light manipulations to test whether sugar import into the phloem is required as the driving force for phloem sap flow. Using carbon-11 radiotracer, we show that a maize sucrose transporter1 (sut1) loss-of-function mutant has severely reduced export of carbon from photosynthetic leaves (only ~4% of the wild type level). Yet, the mutant remarkably maintains phloem pressure at ~100% and sap flow speeds at ~50–75% of those of wild type. Potassium (K+) abundance in the phloem was elevated in sut1 mutant leaves. Fluid dynamic modelling supports the conclusion that increased K+ loading compensated for decreased sucrose loading to maintain phloem pressure, and thereby maintained phloem transport via the pressure-flow mechanism. Furthermore, these results suggest that sap flow and transport of other phloem-mobile nutrients and signalling molecules could be regulated independently of sugar loading into the phloem, potentially influencing carbon–nutrient homoeostasis and the distribution of signalling molecules in plants encountering different environmental conditions.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: German Research Foundation; Independent Research Fund Denmark; National Science Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER); United States Department of Agriculture; Villum Fonden

Grant/Contract Number:: AC02-98CH10886; SC0012704

OSTI ID:: 1887533

Report Number(s):: BNL-223297-2022-JAAM

Journal Information:: Nature Plants (Online), Journal Name: Nature Plants (Online) Journal Issue: 2 Vol. 8; ISSN 2055-0278

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

References (80)

Evidence for an essential role of the sucrose transporter in phloem loading and assimilate partitioning. Riesmeier, J. W.; Willmitzer, L.; Frommer, W. B. The EMBO Journal, Vol. 13, Issue 1 https://doi.org/10.1002/j.1460-2075.1994.tb06229.x	journal	January 1994
Osmoregulation and the control of phloem-sap composition in Ricinus communis L. Smith, J. Andrew C.; Milburn, John A. Planta, Vol. 148, Issue 1 https://doi.org/10.1007/BF00385438	journal	February 1980
Leaf structure in relation to solute transport and phloem loading in Zea mays L. Evert, R. F.; Eschrich, W.; Heyser, W. Planta, Vol. 138, Issue 3 https://doi.org/10.1007/BF00386823	journal	January 1978
Structure and function of leaf minor veins in trees and herbs: A taxonomic review Gamalei, Yuri Trees, Vol. 3, Issue 2 https://doi.org/10.1007/BF01021073	journal	January 1989
Loss of the AKT2/3 potassium channel affects sugar loading into the phloem of Arabidopsis Deeken, Rosalia; Geiger, Dietmar; Fromm, Jörg Planta, Vol. 216, Issue 2 https://doi.org/10.1007/s00425-002-0895-1	journal	December 2002
Subcellular concentrations of sugar alcohols and sugars in relation to phloem translocation in Plantago major, Plantago maritima, Prunus persica, and Apium graveolens Nadwodnik, Jan; Lohaus, Gertrud Planta, Vol. 227, Issue 5 https://doi.org/10.1007/s00425-007-0682-0	journal	January 2008
Partitioning of New Carbon as 11C in Nicotiana tabacum Reveals Insight into Methyl Jasmonate Induced Changes in Metabolism Hanik, Nils; Gómez, Sara; Best, Marcel Journal of Chemical Ecology, Vol. 36, Issue 10 https://doi.org/10.1007/s10886-010-9835-x	journal	September 2010
Salivary proteins of aphids, a pilot study on identification, separation and immunolocalisation Cherqui, Anas; Tjallingii, W. Fred Journal of Insect Physiology, Vol. 46, Issue 8 https://doi.org/10.1016/S0022-1910(00)00037-8	journal	August 2000
Systemic signaling during plant defense Kachroo, Aardra; Robin, Guillaume P. Current Opinion in Plant Biology, Vol. 16, Issue 4 https://doi.org/10.1016/j.pbi.2013.06.019	journal	August 2013
Current perspectives on the regulation of whole-plant carbohydrate partitioning Slewinski, Thomas L.; Braun, David M. Plant Science, Vol. 178, Issue 4 https://doi.org/10.1016/j.plantsci.2010.01.010	journal	April 2010
AtSUC2 has a role for sucrose retrieval along the phloem pathway: Evidence from carbon-11 tracer studies Gould, N.; Thorpe, M. R.; Pritchard, J. Plant Science, Vol. 188-189 https://doi.org/10.1016/j.plantsci.2011.12.018	journal	June 2012
Signaling mechanisms underlying systemic acquired resistance to microbial pathogens Shine, M. B.; Xiao, Xueqiong; Kachroo, Pradeep Plant Science, Vol. 279 https://doi.org/10.1016/j.plantsci.2018.01.001	journal	February 2019
In vivo transport of three radioactive [18F]-fluorinated deoxysucrose analogs by the maize sucrose transporter ZmSUT1 Tran, Thu M.; Hampton, Carissa S.; Brossard, Tom W. Plant Physiology and Biochemistry, Vol. 115 https://doi.org/10.1016/j.plaphy.2017.03.006	journal	June 2017
Aphid saliva Miles, Peter W. Biological Reviews of the Cambridge Philosophical Society, Vol. 74, Issue 1 https://doi.org/10.1017/S0006323198005271	journal	February 1999
Osmotically driven pipe flows and their relation to sugar transport in plants Jensen, KÅRe H.; Rio, Emmanuelle; Hansen, Rasmus Journal of Fluid Mechanics, Vol. 636 https://doi.org/10.1017/S002211200900799X	journal	September 2009
Shoot-to-root mobile polypeptides involved in systemic regulation of nitrogen acquisition Ohkubo, Yuri; Tanaka, Mina; Tabata, Ryo Nature Plants, Vol. 3, Issue 4 https://doi.org/10.1038/nplants.2017.29	journal	March 2017
Passive phloem loading and long-distance transport in a synthetic tree-on-a-chip Comtet, Jean; Jensen, Kaare H.; Turgeon, Robert Nature Plants, Vol. 3, Issue 4 https://doi.org/10.1038/nplants.2017.32	journal	March 2017
Phloem hydrostatic pressure relates to solute loading rate: a direct test of the Münch hypothesis Gould, Nick; Thorpe, Michael R.; Koroleva, Olga Functional Plant Biology, Vol. 32, Issue 11 https://doi.org/10.1071/FP05036	journal	January 2005
Molecular sabotage of plant defense by aphid saliva Will, Torsten; Tjallingii, W. Fred; Thönnessen, Alexandra Proceedings of the National Academy of Sciences, Vol. 104, Issue 25 https://doi.org/10.1073/pnas.0703535104	journal	June 2007
A comprehensive picture of phloem loading strategies Rennie, E. A.; Turgeon, R. Proceedings of the National Academy of Sciences, Vol. 106, Issue 33 https://doi.org/10.1073/pnas.0902279106	journal	July 2009
Starch as a major integrator in the regulation of plant growth Sulpice, R.; Pyl, E. -T.; Ishihara, H. Proceedings of the National Academy of Sciences, Vol. 106, Issue 25 https://doi.org/10.1073/pnas.0903478106	journal	June 2009
Potassium (K+) gradients serve as a mobile energy source in plant vascular tissues Gajdanowicz, P.; Michard, E.; Sandmann, M. Proceedings of the National Academy of Sciences, Vol. 108, Issue 2 https://doi.org/10.1073/pnas.1009777108	journal	December 2010
Sugar demand, not auxin, is the initial regulator of apical dominance Mason, M. G.; Ross, J. J.; Babst, B. A. Proceedings of the National Academy of Sciences, Vol. 111, Issue 16 https://doi.org/10.1073/pnas.1322045111	journal	April 2014
Genetic evidence for the in planta role of phloem-specific plasma membrane sucrose transporters Gottwald, J. R.; Krysan, P. J.; Young, J. C. Proceedings of the National Academy of Sciences, Vol. 97, Issue 25 https://doi.org/10.1073/pnas.250473797	journal	November 2000
The absence of phloem loading in willow leaves Turgeon, R.; Medville, R. Proceedings of the National Academy of Sciences, Vol. 95, Issue 20 https://doi.org/10.1073/pnas.95.20.12055	journal	September 1998
The K+ Channel KZM1 Mediates Potassium Uptake into the Phloem and Guard Cells of the C4 Grass Zea mays Philippar, Katrin; Büchsenschütz, Kai; Abshagen, Maike Journal of Biological Chemistry, Vol. 278, Issue 19 https://doi.org/10.1074/jbc.M212720200	journal	May 2003
Using synchrotron X-ray fluorescence microprobes in the study of metal homeostasis in plants Punshon, Tracy; Guerinot, Mary Lou; Lanzirotti, Antonio Annals of Botany, Vol. 103, Issue 5 https://doi.org/10.1093/aob/mcn264	journal	January 2009
The gelatinous extracellular matrix facilitates transport studies in kelp: visualization of pressure-induced flow reversal across sieve plates Knoblauch, Jan; Peters, Winfried S.; Knoblauch, Michael Annals of Botany, Vol. 117, Issue 4 https://doi.org/10.1093/aob/mcw007	journal	February 2016
Solute balance of a maize (Zea mays L.) source leaf as affected by salt treatment with special emphasis on phloem retranslocation and ion leaching Lohaus, Gertrud; Hussmann, Melanie; Pennewiss, Kerstin Journal of Experimental Botany, Vol. 51, Issue 351 https://doi.org/10.1093/jexbot/51.351.1721	journal	October 2000
The Translocation of¹¹C-labelled Photosynthate in the Soybean Moorby, J.; Ebert, M.; Evans, N. T. S. Journal of Experimental Botany, Vol. 14, Issue 2 https://doi.org/10.1093/jxb/14.2.210	journal	January 1963
Investigations of Carbon Transport in Plants Moorby, J.; Troughton, J. H.; Currie, B. G. Journal of Experimental Botany, Vol. 25, Issue 5 https://doi.org/10.1093/jxb/25.5.937	journal	January 1974
Input–output analysis of in vivo photoassimilate translocation using Positron-Emitting Tracer Imaging System (PETIS) data Keutgen, Anna J.; Keutgen, Norbert; Matsuhashi, Shinpei Journal of Experimental Botany, Vol. 56, Issue 415 https://doi.org/10.1093/jxb/eri143	journal	April 2005
Sucrose transporter1 functions in phloem loading in maize leaves Slewinski, Thomas L.; Meeley, Robert; Braun, David M. Journal of Experimental Botany, Vol. 60, Issue 3 https://doi.org/10.1093/jxb/ern335	journal	January 2009
Polar auxin transport in relation to long-distance transport of nutrients in the Charales: Table 1. Raven, John A. Journal of Experimental Botany, Vol. 64, Issue 1 https://doi.org/10.1093/jxb/ers358	journal	December 2012
Understanding and manipulating sucrose phloem loading, unloading, metabolism, and signalling to enhance crop yield and food security Braun, D. M.; Wang, L.; Ruan, Y. -L. Journal of Experimental Botany, Vol. 65, Issue 7 https://doi.org/10.1093/jxb/ert416	journal	December 2013
Membrane-Transport Systems for Sucrose in Relation to Whole-Plant Carbon Partitioning Ayre, Brian G. Molecular Plant, Vol. 4, Issue 3 https://doi.org/10.1093/mp/ssr014	journal	May 2011
Radio-Metabolite Analysis of Carbon-11 Biochemical Partitioning to Non-Structural Carbohydrates for Integrated Metabolism and Transport Studies Babst, Benjamin A.; Karve, Abhijit A.; Judt, Tatjana Plant and Cell Physiology, Vol. 54, Issue 6 https://doi.org/10.1093/pcp/pct045	journal	April 2013
Sugar Transporters in Plants: New Insights and Discoveries Julius, Benjamin T.; Leach, Kristen A.; Tran, Thu M. Plant and Cell Physiology, Vol. 58, Issue 9 https://doi.org/10.1093/pcp/pcx090	journal	June 2017
Signal Signature and Transcriptome Changes of Arabidopsis During Pathogen and Insect Attack De Vos, Martin; Van Oosten, Vivian R.; Van Poecke, Remco M. P. Molecular Plant-Microbe Interactions®, Vol. 18, Issue 9 https://doi.org/10.1094/MPMI-18-0923	journal	September 2005
Convergent evolution of vascular optimization in kelp (Laminariales) Drobnitch, Sarah Tepler; Jensen, Kaare H.; Prentice, Paige Proceedings of the Royal Society B: Biological Sciences, Vol. 282, Issue 1816 https://doi.org/10.1098/rspb.2015.1667	journal	October 2015
Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H ⁺ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. Plant Physiology, Vol. 148, Issue 1 https://doi.org/10.1104/pp.108.124776	journal	July 2008
Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading Braun, David M.; Slewinski, Thomas L. Plant Physiology, Vol. 149, Issue 1 https://doi.org/10.1104/pp.108.129049	journal	January 2009
Tie-dyed1 Encodes a Novel, Phloem-Expressed Transmembrane Protein That Functions in Carbohydrate Partitioning Ma, Yi; Slewinski, Thomas L.; Baker, R. Frank Plant Physiology, Vol. 149, Issue 1 https://doi.org/10.1104/pp.108.130971	journal	October 2008
The Role of Phloem Loading Reconsidered Turgeon, Robert Plant Physiology, Vol. 152, Issue 4 https://doi.org/10.1104/pp.110.153023	journal	March 2010
The Puzzle of Phloem Pressure Turgeon, Robert Plant Physiology, Vol. 154, Issue 2 https://doi.org/10.1104/pp.110.161679	journal	October 2010
The Role of CAX1 and CAX3 in Elemental Distribution and Abundance in Arabidopsis Seed Punshon, Tracy; Hirschi, Kendal; Yang, Jian Plant Physiology, Vol. 158, Issue 1 https://doi.org/10.1104/pp.111.184812	journal	November 2011
Phloem Loading Strategies and Water Relations in Trees and Herbaceous Plants Fu, Qiushi; Cheng, Lailiang; Guo, Yangdong Plant Physiology, Vol. 157, Issue 3 https://doi.org/10.1104/pp.111.184820	journal	August 2011
Pico Gauges for Minimally Invasive Intracellular Hydrostatic Pressure Measurements Knoblauch, J.; Mullendore, D. L.; Jensen, K. H. PLANT PHYSIOLOGY, Vol. 166, Issue 3 https://doi.org/10.1104/pp.114.245746	journal	September 2014
Symplastic Phloem Loading in Poplar Zhang, Cankui; Han, Lu; Slewinski, Thomas L. Plant Physiology, Vol. 166, Issue 1 https://doi.org/10.1104/pp.114.245845	journal	July 2014
The H ⁺ -Sucrose Cotransporter NtSUT1 Is Essential for Sugar Export from Tobacco Leaves Bürkle, Lukas; Hibberd, Julian M.; Quick, W. Paul Plant Physiology, Vol. 118, Issue 1 https://doi.org/10.1104/pp.118.1.59	journal	September 1998
Sucrose Transporter ZmSut1 Expression and Localization Uncover New Insights into Sucrose Phloem Loading Baker, R. Frank; Leach, Kristen A.; Boyer, Nathanial R. Plant Physiology, Vol. 172, Issue 3 https://doi.org/10.1104/pp.16.00884	journal	September 2016
Synchrotron-Based X-Ray Fluorescence Microscopy as a Technique for Imaging of Elements in Plants Kopittke, Peter M.; Punshon, Tracy; Paterson, David J. Plant Physiology, Vol. 178, Issue 2 https://doi.org/10.1104/pp.18.00759	journal	August 2018
Relations between Light Level, Sucrose Concentration, and Translocation of Carbon 11 in Zea mays Leaves Troughton, John H.; Currie, B. G.; Chang, F. H. Plant Physiology, Vol. 59, Issue 5 https://doi.org/10.1104/pp.59.5.808	journal	May 1977
Sieve Tube Geometry in Relation to Phloem Flow Mullendore, Daniel L.; Windt, Carel W.; Van As, Henk The Plant Cell, Vol. 22, Issue 3 https://doi.org/10.1105/tpc.109.070094	journal	March 2010
Network Analysis of Enzyme Activities and Metabolite Levels and Their Relationship to Biomass in a Large Panel of Arabidopsis Accessions Sulpice, Ronan; Trenkamp, Sandra; Steinfath, Matthias The Plant Cell, Vol. 22, Issue 8 https://doi.org/10.1105/tpc.110.076653	journal	August 2010
Aphid responses to plants with genetically manipulated phloem nutrient levels Pescod, K. V.; Quick, W. P.; Douglas, A. E. Physiological Entomology, Vol. 32, Issue 3 https://doi.org/10.1111/j.1365-3032.2007.00577.x	journal	September 2007
Ecophysiology of phloem loading in source leaves Bel, A. J. E.; Gamalei, Y. V. Plant, Cell and Environment, Vol. 15, Issue 3 https://doi.org/10.1111/j.1365-3040.1992.tb00973.x	journal	April 1992
Coordination of carbon supply and plant growth Smith, Alison M.; Stitt, Mark Plant, Cell & Environment, Vol. 30, Issue 9 https://doi.org/10.1111/j.1365-3040.2007.01708.x	journal	September 2007
Universality of phloem transport in seed plants: Universality of phloem transport in seed plants Jensen, KÅRe Hartvig; Liesche, Johannes; Bohr, Tomas Plant, Cell & Environment, Vol. 35, Issue 6 https://doi.org/10.1111/j.1365-3040.2011.02472.x	journal	January 2012
Jasmonic acid induces rapid changes in carbon transport and partitioning in Populus Babst, Benjamin A.; Ferrieri, Richard A.; Gray, Dennis W. New Phytologist, Vol. 167, Issue 1 https://doi.org/10.1111/j.1469-8137.2005.01388.x	journal	March 2005
Lymantria dispar herbivory induces rapid changes in carbon transport and partitioning in Populus nigra Babst, Benjamin A.; Ferrieri, Richard A.; Thorpe, Michael R. Entomologia Experimentalis et Applicata, Vol. 128, Issue 1 https://doi.org/10.1111/j.1570-7458.2008.00698.x	journal	May 2008
The Plant Vascular System: Evolution, Development and Functions ^F Lucas, William J.; Groover, Andrew; Lichtenberger, Raffael Journal of Integrative Plant Biology, Vol. 55, Issue 4 https://doi.org/10.1111/jipb.12041	journal	April 2013
The potassium battery: a mobile energy source for transport processes in plant vascular tissues Dreyer, Ingo; Gomez‐Porras, Judith Lucia; Riedelsberger, Janin New Phytologist, Vol. 216, Issue 4 https://doi.org/10.1111/nph.14667	journal	June 2017
In situmicroscopy reveals reversible cell wall swelling in kelp sieve tubes: one mechanism for turgor generation and flow control? Knoblauch, Jan; Tepler Drobnitch, Sarah; Peters, Winfried S. Plant, Cell & Environment, Vol. 39, Issue 8 https://doi.org/10.1111/pce.12736	journal	May 2016
Methyl Salicylate Is a Critical Mobile Signal for Plant Systemic Acquired Resistance Park, S. -W.; Kaimoyo, E.; Kumar, D. Science, Vol. 318, Issue 5847 https://doi.org/10.1126/science.1147113	journal	October 2007
Priming in Systemic Plant Immunity Jung, Ho Won; Tschaplinski, Timothy J.; Wang, Lin Science, Vol. 324, Issue 5923 https://doi.org/10.1126/science.1170025	journal	April 2009
Phloem-Mobile RNAs as Systemic Signaling Agents Ham, Byung-Kook; Lucas, William J. Annual Review of Plant Biology, Vol. 68, Issue 1 https://doi.org/10.1146/annurev-arplant-042916-041139	journal	April 2017
Phloem Loading of Sucrose Giaquinta, R. T. Annual Review of Plant Physiology, Vol. 34, Issue 1 https://doi.org/10.1146/annurev.pp.34.060183.002023	journal	June 1983
Sucrose accumulation in sweet sorghum stems occurs by apoplasmic phloem unloading and does not involve differential Sucrose transporter expression Bihmidine, Saadia; Baker, R. Frank; Hoffner, Cassandra BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0572-8	journal	July 2015
In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner Karve, Abhijit A.; Alexoff, David; Kim, Dohyun BMC Plant Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12870-015-0658-3	journal	November 2015
Plant cues for aphid navigation in vascular tissues Hewer, Angela; Will, Torsten; van Bel, Aart J. E. Journal of Experimental Biology, Vol. 213, Issue 23 https://doi.org/10.1242/jeb.046326	journal	December 2010
An aphid's Odyssey – the cortical quest for the vascular bundle Hewer, Angela; Becker, Alexander; van Bel, Aart J. E. Journal of Experimental Biology, Vol. 214, Issue 22 https://doi.org/10.1242/jeb.060913	journal	November 2011
Radiosynthesis of 6’-Deoxy-6’[18F]Fluorosucrose via Automated Synthesis and Its Utility to Study In Vivo Sucrose Transport in Maize (Zea mays) Leaves Rotsch, David; Brossard, Tom; Bihmidine, Saadia PLOS ONE, Vol. 10, Issue 5 https://doi.org/10.1371/journal.pone.0128989	journal	May 2015
Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using 11CO2 and a Positron-emitting Tracer Imaging System Kikuchi, Kaori; Ishii, Satomi; Fujimaki, Shu Journal of the Japanese Society for Horticultural Science, Vol. 77, Issue 2 https://doi.org/10.2503/jjshs1.77.199	journal	January 2008
Does Don Fisher's high-pressure manifold model account for phloem transport and resource partitioning? Patrick, John W. Frontiers in Plant Science, Vol. 4 https://doi.org/10.3389/fpls.2013.00184	journal	January 2013
Structural and functional heterogeneity in phloem loading and transport Slewinski, Thomas L.; Zhang, Cankui; Turgeon, Robert Frontiers in Plant Science, Vol. 4 https://doi.org/10.3389/fpls.2013.00244	journal	January 2013
Small RNA Regulators of Plant-Hemipteran Interactions: Micromanagers with Versatile Roles Sattar, Sampurna; Thompson, Gary A. Frontiers in Plant Science, Vol. 7 https://doi.org/10.3389/fpls.2016.01241	journal	August 2016
Maize SUT1 functions in phloem loading Slewinski, Thomas L.; Garg, Anshu; Johal, Gurmukh S. Plant Signaling & Behavior, Vol. 5, Issue 6 https://doi.org/10.4161/psb.5.6.11575	journal	June 2010
In vivo quantitative imaging of photoassimilate transport dynamics and allocation in large plants using a commercial positron emission tomography (PET) scanner [Supplemental Data] Alexoff, David; Schueller, Michael; Ferrieri, Richard figshare-Supplementary information for journal article at DOI: 10.1186/s12870-015-0658-3, 3 files https://doi.org/10.6084/m9.figshare.c.3619742	collection	November 2015
Testing the Münch hypothesis of long distance phloem transport in plants Knoblauch, Michael; Knoblauch, Jan; Mullendore, Daniel L. eLife, Vol. 5 https://doi.org/10.7554/eLife.15341	journal	June 2016

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