Functional characterization and regulatory modeling of lignocellulose deconstruction in the saprophytic bacterium Cellvibrio japonicus (Final Technical Report)
- Univ. of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
The goal of this project was to establish a fundamental systems-level model of lignocellulose deconstruction by saprophytic soil bacteria while simultaneously identifying and characterizing novel enzymes that can be used for applied bioenergy technologies. This project was significant because it elevated our mechanistic and regulatory understanding of bacterial plant biomass degradation and led to technologies to enable bottlenecks in biofuel production R&D. Furthermore, the completed work was novel because it integrated in vivo, in vitro, and in silico approaches with bioenergy-relevant substrates. We identified the required enzymes for lignocellulose degradation and mechanisms of regulation through a combination of transcriptomic profiling (RNAseq), enzymatic analysis, the functional characterization of mutants, and modeling of the regulatory networks used to detect and degrade lignocellulose. This project employed the lignocellulose-degrading bacterium Cellvibrio japonicus to dissect the functional properties and underlying regulatory networks saprophytic bacteria use to depolymerize and utilize lignocellulose. There were four Objectives to this project, the specific metrics of which are summarized in this report.
- Research Organization:
- University of Maryland Baltimore County (UMBC), Baltimore, MD (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- SC0014183
- OSTI ID:
- 1884807
- Report Number(s):
- DOE-UMBC-14183
- Country of Publication:
- United States
- Language:
- English
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