A combination of deep eutectic solvent and ethanol pretreatment for synergistic delignification and enhanced enzymatic hydrolysis for biorefinary process

Yao, Lan; Cui, Pingping; Chen, Xiong; Yoo, Chang Geun; Liu, Qianting; Meng, Xianzhi; Xiong, Long; Ragauskas, Arthur J.; Yang, Haitao

doi:10.1016/j.biortech.2022.126885

A combination of deep eutectic solvent and ethanol pretreatment for synergistic delignification and enhanced enzymatic hydrolysis for biorefinary process

Journal Article · Mon Feb 21 23:00:00 EST 2022 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2022.126885· OSTI ID:1883801

Yao, Lan ^[1]; Cui, Pingping ^[1]; Chen, Xiong ^[1]; Yoo, Chang Geun ^[2]; Liu, Qianting ^[1]; Meng, Xianzhi ^[3]; Xiong, Long ^[1]; ^[4]; Yang, Haitao ^[5]

Hubei University of Technology, Wuhan (China)
State University of New York (SUNY), Syracuse, NY (United States); Michael M. Szwarc Polymer Research Institute, Syracuse, NY (United States)
University of Tennessee, Knoxville, TN (United States)
University of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); The University of Tennessee Knoxville, Institute of Agriculture, Knoxville, TN (United States)
Hubei University of Technology, Wuhan (China); Qilu Univesity of Technology, Shandong (China)

Here, a novel pretreatment system containing deep eutectic solvents and ethanol (DES-E) for synergistic carbohydrate conversion and delignification was reported in this study. The DES-E pretreatment resulted in an enhanced glucose yield compared to individual DES and ethanol pretreatment for the three tested biomass, including Broussonetia papyrifera, corn stover and pine. To further explore the delignification mechanism, the solubilized lignin and residual lignin from Broussonetia papyrifera was recovered and extracted, then thoroughly characterized. The highest total OH content was found in the DES-E solubilized lignin, which could be used as antioxidant. The presence of ethanol in pretreatment liquor could protect the β-O-4 substructure from breakage and reduce lignin condensation, which favors the subsequent enzymatic hydrolysis. Comparable glucose yield and delignification performance was achieved by recycled DES. DES-E pretreatment offers a promising method for lignin isolation and cellulose digestibility improvement simultaneously.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Natural Science Foundation of China; China Scholarship Council; Hubei Provincial Department of Education

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1883801

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: 1 Vol. 350; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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