Enhancing Enzyme-Mediated Hydrolysis of Mechanical Pulps by Deacetylation and Delignification

Wu, Jie; Chandra, Richard P.; Kim, Kwang Ho; Kim, Chang Soo; Pu, Yunqiao Joseph; Ragauskas, Arthur J.; Saddler, Jack Nicholas

doi:10.1021/acssuschemeng.9b07226

Title: Enhancing Enzyme-Mediated Hydrolysis of Mechanical Pulps by Deacetylation and Delignification

Journal Article · Tue Mar 24 00:00:00 EDT 2020 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.9b07226· OSTI ID:1619019

Wu, Jie ^[1];

^[1];

^[2]; Kim, Chang Soo ^[2];

^[3];

^[4];

^[1]

Univ. of British Columbia, Vancouver, BC (Canada)
Korea Inst. of Science and Technology, Seoul (Republic of Korea)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, Center for Bioenergy Innovation, Joint Inst. of Biological Science
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division, Center for Bioenergy Innovation, Joint Inst. of Biological Science; Univ. of Tennessee, Knoxville, TN (United States)

Alkaline induced deacetylation of the hemicellulose combined with subsequent mechanical refining enhanced the enzyme-mediated hydrolysis of pretreated corn stover. The addition of either NaOH (80 °C) or mild KOH (25 °C) to corn stover prior to mechanical refining led to greater than 80% deacetylation with the NaOH treatment also solubilizing low-molecular-weight lignin that was enriched in $$β$$-O-4 linkages with more than 25% and 13% of the total and surface lignin removed, respectively. Furthermore, the influence of deacetylation and delignification were further enhanced when NaOH was supplemented with 3% Na₂SO₃, resulting in 100% deacetylation, 34% delignification, and a >20% increase in the hydrolysis yield of the substrate xylan. A milder KOH treatment resulted in the retention of more than 95% of the lignin within the cellulose rich, water-insoluble fraction with no apparent change in the surface lignin. However, both methods resulted in enhanced xylan hydrolysis when treated with xylanases, suggesting that deacetylation had enhanced accessibility to the xylan present in the pretreated of corn stover. It was apparent that cellulose accessibility was also enhanced by partial delignification, as NaOH treatment resulted in a 65% and 43% increase in the Water Retention Value and Directed Orange dye adsorption, respectively.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; Natural Sciences and Engineering Research Council of Canada (NSERC); Korea Institute of Science and Technology (KIST); Novozymes

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1619019

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 15; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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