Revealing the mechanism of surfactant-promoted enzymatic hydrolysis of dilute acid pretreated bamboo

Huang, Caoxing; Zhao, Xiaoxue; Zheng, Yayue; Lin, Wenqian; Lai, Chenhuan; Yong, Qiang; Ragauskas, Arthur J.; Meng, Xianzhi

doi:10.1016/j.biortech.2022.127524

Revealing the mechanism of surfactant-promoted enzymatic hydrolysis of dilute acid pretreated bamboo

Journal Article · Sat Jun 25 00:00:00 EDT 2022 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2022.127524· OSTI ID:1883788

Huang, Caoxing ^[1]; Zhao, Xiaoxue ^[1]; Zheng, Yayue ^[1]; Lin, Wenqian ^[1]; Lai, Chenhuan ^[1]; Yong, Qiang ^[1]; ^[2]; Meng, Xianzhi ^[3]

Nanjing Forestry University (China)
University of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
University of Tennessee, Knoxville, TN (United States)

Here, to improve the enzymatic digestibility of dilute acid pretreated bamboo residue (DABR), surfactants including PEG 4000 and Tween 80 were added to prevent the non-productive adsorption between residual lignin and enzyme. At the optimal loadings (e.g., 0.2 and 0.3 g surfactant/g lignin), the enzymatic digestibility of DABR improved from 29.4% to 64.6% and 61.6% for PEG 4000 and Tween 80, respectively. Furthermore, the promoting mechanism of these surfactants on enzymatic hydrolysis was investigated by real-time surface plasmon resonance (SPR) and fluorescence spectroscopy. Results from SPR analysis showed that Tween 80 outperformed PEG 4000 in terms of dissociating the irreversible cellulase adsorption onto lignin. Fluorescence quenching mechanism revealed that PEG 4000 and Tween 80 intervened the interaction between lignin and cellulase by hydrogen bonds/Van der Waals and hydrophobic action, respectively. This work provided an in-depth understanding of the mechanisms of PEG 4000 and Tween 80 on enhancing the enzymatic hydrolysis efficiency.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: Jiangsu Qing Lan Project; USDOE; University of Tennessee

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1883788

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: 1 Vol. 360; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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