Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Using FLIC Microscopy [Book Chapter]

VanDelinder, Virginia Alison; Bachand, George D.

doi:10.1007/978-1-0716-1983-4_6

Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Using FLIC Microscopy [Book Chapter]

Journal Article · Thu Apr 28 00:00:00 EDT 2022 · Methods in Molecular Biology

DOI:https://doi.org/10.1007/978-1-0716-1983-4_6· OSTI ID:1883173

VanDelinder, Virginia Alison ^[1]; Bachand, George D. ^[1]

Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies (CINT)

Intracellular transport by kinesin motors moving along their associated cytoskeletal filaments, microtubules, is essential to many biological processes. This active transport system can be reconstituted in vitro with the surface-adhered motors transporting the microtubules across a planar surface. In this geometry, the kinesin-microtubule system has been used to study active self-assembly, to power microdevices, and to perform analyte detection. Fundamental to these applications is the ability to characterize the interactions between the surface tethered motors and microtubules. Further, Fluorescence Interference Contrast (FLIC) microscopy can illuminate the height of the microtubule above a surface, which, at sufficiently low surface densities of kinesin, also reveals the number, locations, and dynamics of the bound motors.

View Accepted Manuscript (DOE)

Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies (CINT)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division; USDOE National Nuclear Security Administration (NNSA)

Grant/Contract Number:: NA0003525

OSTI ID:: 1883173

Report Number(s):: SAND2021-0734J; 693561

Journal Information:: Methods in Molecular Biology, Journal Name: Methods in Molecular Biology Vol. 2430; ISSN 1064-3745

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (19)

Quantifying the Performance of Protein-Resisting Surfaces at Ultra-Low Protein Coverages using Kinesin Motor Proteins as Probes Katira, P.; Agarwal, A.; Fischer, T. Advanced Materials, Vol. 19, Issue 20 https://doi.org/10.1002/adma.200701982	journal	October 2007
Powering Nanodevices with Biomolecular Motors Hess, Henry; Bachand, George D.; Vogel, Viola Chemistry - A European Journal, Vol. 10, Issue 9 https://doi.org/10.1002/chem.200305712	journal	May 2004
3D Nanometer Tracking of Motile Microtubules on Reflective Surfaces Kerssemakers, Jacob; Ionov, Leonid; Queitsch, Ute Small, Vol. 5, Issue 15 https://doi.org/10.1002/smll.200801388	journal	August 2009
Biomolecular motors in nanoscale materials, devices, and systems: Biomolecular motors in nanoscale materials, devices, and systems Bachand, George D.; Bouxsein, Nathan F.; VanDelinder, Virginia Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, Vol. 6, Issue 2 https://doi.org/10.1002/wnan.1252	journal	December 2013
Fluorescence interference-contrast microscopy on oxidized silicon using a monomolecular dye layer Lambacher, Armin; Fromherz, Peter Applied Physics A Materials Science & Processing, Vol. 63, Issue 3 https://doi.org/10.1007/BF01567871	journal	September 1996
Towards the application of cytoskeletal motor proteins in molecular detection and diagnostic devices Korten, Till; Månsson, Alf; Diez, Stefan Current Opinion in Biotechnology, Vol. 21, Issue 4 https://doi.org/10.1016/j.copbio.2010.05.001	journal	August 2010
Engineering with Biomolecular Motors Hess, Henry; Saper, Gadiel Accounts of Chemical Research, Vol. 51, Issue 12 https://doi.org/10.1021/acs.accounts.8b00296	journal	October 2018
Synthetic Systems Powered by Biological Molecular Motors Saper, Gadiel; Hess, Henry Chemical Reviews, Vol. 120, Issue 1 https://doi.org/10.1021/acs.chemrev.9b00249	journal	August 2019
On the Mechanism of Trolox as Antiblinking and Antibleaching Reagent Cordes, Thorben; Vogelsang, Jan; Tinnefeld, Philip Journal of the American Chemical Society, Vol. 131, Issue 14 https://doi.org/10.1021/ja809117z	journal	April 2009
Spontaneous motion in hierarchically assembled active matter Sanchez, Tim; Chen, Daniel T. N.; DeCamp, Stephen J. Nature, Vol. 491, Issue 7424 https://doi.org/10.1038/nature11591	journal	November 2012
Orientational order of motile defects in active nematics DeCamp, Stephen J.; Redner, Gabriel S.; Baskaran, Aparna Nature Materials, Vol. 14, Issue 11 https://doi.org/10.1038/nmat4387	journal	August 2015
Kinesin motor density and dynamics in gliding microtubule motility VanDelinder, Virginia; Imam, Zachary I.; Bachand, George Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-43749-8	journal	May 2019
Directed self-assembly of 1D microtubule nano-arrays Bachand, M.; Bouxsein, N. F.; Cheng, S. RSC Adv., Vol. 4, Issue 97 https://doi.org/10.1039/C4RA11765D	journal	January 2014
Cytoskeletal motor-driven active self-assembly in in vitro systems Lam, A. T.; VanDelinder, V.; Kabir, A. M. R. Soft Matter, Vol. 12, Issue 4 https://doi.org/10.1039/C5SM02042E	journal	January 2016
Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots Hess, H.; Ross, Jennifer L. Chemical Society Reviews, Vol. 46, Issue 18 https://doi.org/10.1039/c7cs00030h	journal	January 2017
The distance that kinesin-1 holds its cargo from the microtubule surface measured by fluorescence interference contrast microscopy Kerssemakers, J.; Howard, J.; Hess, H. Proceedings of the National Academy of Sciences, Vol. 103, Issue 43 https://doi.org/10.1073/pnas.0510400103	journal	October 2006
Kinesin Takes One 8-nm Step for Each ATP That It Hydrolyzes Coy, David L.; Wagenbach, Michael; Howard, Jonathon Journal of Biological Chemistry, Vol. 274, Issue 6 https://doi.org/10.1074/jbc.274.6.3667	journal	February 1999
An unbiased detector of curvilinear structures Steger, C. IEEE Transactions on Pattern Analysis and Machine Intelligence, Vol. 20, Issue 2 https://doi.org/10.1109/34.659930	journal	January 1998
Kinesin-1 Expressed in Insect Cells Improves Microtubule in Vitro Gliding Performance, Long-Term Stability and Guiding Efficiency in Nanostructures Korten, Till; Chaudhuri, Samata; Tavkin, Elena IEEE Transactions on NanoBioscience, Vol. 15, Issue 1 https://doi.org/10.1109/TNB.2016.2520832	journal	January 2016

Similar Records

Kinesin motor density and dynamics in gliding microtubule motility

Journal Article · Fri May 10 00:00:00 EDT 2019 · Scientific Reports · OSTI ID:1619514

Mechanical splitting of microtubules into protofilament bundles by surface-bound kinesin-1

Journal Article · Tue Dec 20 23:00:00 EST 2016 · Scientific Reports · OSTI ID:1339254

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FLIC microscopy
Gliding assay
Kinesin
Microtubules

Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay Using FLIC Microscopy [Book Chapter]

Citation Formats

References (19)

Similar Records

Related Subjects