Liquid Crystal‐Based Detection of Antigens with ELISA Sensitivity

Perera, Kelum; Dassanayake, Thiloka M.; Jeewanthi, Mahesha; Haputhanthrige, Nilanthi P.; Huang, Songping D.; Kooijman, Edgar; Mann, Elizabeth; Jákli, Antal

doi:10.1002/admi.202200891

Liquid Crystal‐Based Detection of Antigens with ELISA Sensitivity

Journal Article · Tue Aug 02 00:00:00 EDT 2022 · Advanced Materials Interfaces

DOI:https://doi.org/10.1002/admi.202200891· OSTI ID:1879574

Perera, Kelum ^[1]; Dassanayake, Thiloka M. ^[2]; Jeewanthi, Mahesha ^[1]; Haputhanthrige, Nilanthi P. ^[1]; Huang, Songping D. ^[2]; Kooijman, Edgar ^[3]; Mann, Elizabeth ^[4]; ^[4]

Department of Physics Kent State University Kent OH 44242 USA
Department of Chemistry and Biochemistry Kent State University Kent OH 44242 USA
Department of Biological Sciences Kent State University Kent OH 44242 USA, Advanced Materials and Liquid Crystal Institute Kent State University Kent OH 44242 USA
Department of Physics Kent State University Kent OH 44242 USA, Materials Science Graduate Program Kent State University Kent OH 44242 USA, Advanced Materials and Liquid Crystal Institute Kent State University Kent OH 44242 USA

Abstract

A highly sensitive label‐free liquid crystal (LC)‐based technique is presented for detecting Immunoglobulin G (IgG) antigens used to uncover viral infections. The effectiveness, sensitivity, and selectivity of this detection method is demonstrated with goat IgG antigen at concentrations as low as 100 pg ml ⁻¹ , which is comparable to the sensitivity of the current enzyme‐linked immunosorbent assay (ELISA). The sensor is fabricated by decorating a transmission electron microscopy grid immobilized glass surfaces with antibodies; the target antigen is detected by a liquid crystal suspended onto the grid. This is different from previous methods where the antigen is detected either at the LC‐aqueous interface or in an LC sandwich cell with an antibody/antigen‐decorated substrate. This new approach has advantages such as easy sample preparation, higher sensitivity, and better storage capabilities. Binding the target antigen to the antibody results in a reorientation of the LC director that is detected optically. In addition to demonstrating the sensitivity, the physical principle of the detection is also discussed. This technique may apply to detect virtually any antigen of interest.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0019105

OSTI ID:: 1879574

Alternate ID(s):: OSTI ID: 1885474
OSTI ID: 1976224

Journal Information:: Advanced Materials Interfaces, Journal Name: Advanced Materials Interfaces Journal Issue: 25 Vol. 9; ISSN 2196-7350

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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