Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy

Chakraborty, Arnab; Deligey, Fabien; Quach, Jenny; Mentink-Vigier, Frederic; Wang, Ping; Wang, Tuo

doi:10.1042/bst20191084

Title: Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy

Journal Article · Thu May 07 00:00:00 EDT 2020 · Biochemical Society Transactions

DOI:https://doi.org/10.1042/bst20191084· OSTI ID:1878627

Chakraborty, Arnab ^[1]; Deligey, Fabien ^[1]; Quach, Jenny ^[1]; Mentink-Vigier, Frederic ^[2]; Wang, Ping ^[3];

^[1]

Louisiana State Univ., Baton Rouge, LA (United States)
National High Magnetic Field Lab., Tallahassee, FL (United States)
Louisiana State Univ. Health Sciences Center, New Orleans, LA (United States)

Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing technique magic-angle spinning dynamic nuclear polarization (MAS-DNP) have substantially expanded the territory of ssNMR investigations and enabled the detection of polymer interfaces in a cellular environment. This article highlights the emerging MAS-DNP approaches and their applications to the analysis of biomolecular composites and intact cells to determine the folding pathway and ligand binding of proteins, the structural polymorphism of low-populated biopolymers, as well as the physical interactions between carbohydrates, proteins, and lignin. Furthermore, these structural features provide an atomic-level understanding of many cellular processes, promoting the development of better biomaterials and inhibitors. It is anticipated that the capabilities of MAS-DNP in biomolecular and biomaterial research will be further enlarged by the rapid development of instrumentation and methodology.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Louisiana State Univ., Baton Rouge, LA (United States). Center for Lignocellulose Structure and Formation

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: SC0001090; AI149289; NSF/DMR-1644779

OSTI ID:: 1878627

Journal Information:: Biochemical Society Transactions, Vol. 48, Issue 3; ISSN 0300-5127

Publisher:: Portland PressCopyright Statement

Country of Publication:: United States

Language:: English

References (98)

Structure and Dynamics of Membrane Proteins by Magic Angle Spinning Solid-State NMR McDermott, Ann Annual Review of Biophysics, Vol. 38, Issue 1 https://doi.org/10.1146/annurev.biophys.050708.133719	journal	June 2009
RNA structure determination by solid-state NMR spectroscopy Marchanka, Alexander; Simon, Bernd; Althoff-Ospelt, Gerhard Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms8024	journal	May 2015
Emerging Structural Understanding of Amyloid Fibrils by Solid-State NMR Meier, Beat H.; Riek, Roland; Böckmann, Anja Trends in Biochemical Sciences, Vol. 42, Issue 10 https://doi.org/10.1016/j.tibs.2017.08.001	journal	October 2017
Protein Structure Determination by Magic-Angle Spinning Solid-State NMR, and Insights into the Formation, Structure, and Stability of Amyloid Fibrils Comellas, Gemma; Rienstra, Chad M. Annual Review of Biophysics, Vol. 42, Issue 1 https://doi.org/10.1146/annurev-biophys-083012-130356	journal	May 2013
Structure and Dynamics of Membrane Proteins from Solid-State NMR Mandala, Venkata S.; Williams, Jonathan K.; Hong, Mei Annual Review of Biophysics, Vol. 47, Issue 1 https://doi.org/10.1146/annurev-biophys-070816-033712	journal	May 2018
Magic-angle-spinning NMR spectroscopy applied to small molecules and peptides in lipid bilayers Ader, C.; Schneider, R.; Seidel, K. Biochemical Society Transactions, Vol. 35, Issue 5 https://doi.org/10.1042/BST0350991	journal	October 2007
Solid-state NMR spectroscopy as a tool for drug design: from membrane-embedded targets to amyloid fibrils Middleton, D. A. Biochemical Society Transactions, Vol. 35, Issue 5 https://doi.org/10.1042/BST0350985	journal	October 2007
DNP-Enhanced MAS NMR: A Tool to Snapshot Conformational Ensembles of α-Synuclein in Different States Uluca, Boran; Viennet, Thibault; Petrović, Dušan Biophysical Journal, Vol. 114, Issue 7 https://doi.org/10.1016/j.bpj.2018.02.011	journal	April 2018
Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR Kang, Xue; Kirui, Alex; Dickwella Widanage, Malitha C. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-018-08252-0	journal	January 2019
Molecular architecture of fungal cell walls revealed by solid-state NMR Kang, Xue; Kirui, Alex; Muszyński, Artur Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05199-0	journal	July 2018
Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls Wang, T.; Park, Y. B.; Caporini, M. A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 41 https://doi.org/10.1073/pnas.1316290110	journal	September 2013
Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus Frederick, Kendra K.; Michaelis, Vladimir K.; Corzilius, Björn Cell, Vol. 163, Issue 3 https://doi.org/10.1016/j.cell.2015.09.024	journal	October 2015
Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents Albert, Brice J.; Gao, Chukun; Sesti, Erika L. Biochemistry, Vol. 57, Issue 31 https://doi.org/10.1021/acs.biochem.8b00257	journal	April 2018
Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization Sergeyev, Ivan V.; Itin, Boris; Rogawski, Rivkah Proceedings of the National Academy of Sciences, Vol. 114, Issue 20 https://doi.org/10.1073/pnas.1701484114	journal	May 2017
Theoretical aspects of Magic Angle Spinning - Dynamic Nuclear Polarization Mentink-Vigier, Frederic; Akbey, Ümit; Oschkinat, Hartmut Journal of Magnetic Resonance, Vol. 258 https://doi.org/10.1016/j.jmr.2015.07.001	journal	September 2015
High Frequency Dynamic Nuclear Polarization Ni, Qing Zhe; Daviso, Eugenio; Can, Thach V. Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar300348n	journal	April 2013
Dynamic Nuclear Polarization Surface Enhanced NMR Spectroscopy Rossini, Aaron J.; Zagdoun, Alexandre; Lelli, Moreno Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar300322x	journal	March 2013
Stability of nitroxide biradical TOTAPOL in biological samples McCoy, Kelsey M.; Rogawski, Rivkah; Stovicek, Olivia Journal of Magnetic Resonance, Vol. 303 https://doi.org/10.1016/j.jmr.2019.04.013	journal	June 2019
Low-temperature dynamic nuclear polarization at 9.4T with a 30mW microwave source Thurber, Kent R.; Yau, Wai-Ming; Tycko, Robert Journal of Magnetic Resonance, Vol. 204, Issue 2 https://doi.org/10.1016/j.jmr.2010.03.016	journal	June 2010
Efficient DNP NMR of membrane proteins: sample preparation protocols, sensitivity, and radical location Liao, Shu Y.; Lee, Myungwoon; Wang, Tuo Journal of Biomolecular NMR, Vol. 64, Issue 3 https://doi.org/10.1007/s10858-016-0023-3	journal	February 2016
Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR Kirui, Alex; Dickwella Widanage, Malitha C.; Mentink-Vigier, Frederic Journal of Visualized Experiments, Issue 144 https://doi.org/10.3791/59152	journal	January 2019
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments Takahashi, Hiroki; Fernández-de-Alba, Carlos; Lee, Daniel Journal of Magnetic Resonance, Vol. 239 https://doi.org/10.1016/j.jmr.2013.12.005	journal	February 2014
TOTAPOL: A Biradical Polarizing Agent for Dynamic Nuclear Polarization Experiments in Aqueous Media Song, Changsik; Hu, Kan-Nian; Joo, Chan-Gyu Journal of the American Chemical Society, Vol. 128, Issue 35 https://doi.org/10.1021/ja061284b	journal	September 2006
Highly Efficient, Water-Soluble Polarizing Agents for Dynamic Nuclear Polarization at High Frequency Sauvée, Claire; Rosay, Melanie; Casano, Gilles Angewandte Chemie International Edition, Vol. 52, Issue 41 https://doi.org/10.1002/anie.201304657	journal	August 2013
Computationally Assisted Design of Polarizing Agents for Dynamic Nuclear Polarization Enhanced NMR: The AsymPol Family Mentink-Vigier, Frédéric; Marin-Montesinos, Ildefonso; Jagtap, Anil P. Journal of the American Chemical Society, Vol. 140, Issue 35 https://doi.org/10.1021/jacs.8b04911	journal	August 2018
Neurotoxin II Bound to Acetylcholine Receptors in Native Membranes Studied by Dynamic Nuclear Polarization NMR Linden, Arne H.; Lange, Sascha; Franks, W. Trent Journal of the American Chemical Society, Vol. 133, Issue 48 https://doi.org/10.1021/ja206999c	journal	December 2011
Fast and accurate MAS–DNP simulations of large spin ensembles Mentink-Vigier, Frédéric; Vega, Shimon; De Paëpe, Gaël Physical Chemistry Chemical Physics, Vol. 19, Issue 5 https://doi.org/10.1039/C6CP07881H	journal	January 2017
A Method for Dynamic Nuclear Polarization Enhancement of Membrane Proteins Smith, Adam N.; Caporini, Marc A.; Fanucci, Gail E. Angewandte Chemie International Edition, Vol. 54, Issue 5 https://doi.org/10.1002/anie.201410249	journal	December 2014
Selective high-resolution DNP-enhanced NMR of biomolecular binding sites Marin-Montesinos, Ildefonso; Goyard, David; Gillon, Emilie Chemical Science, Vol. 10, Issue 11 https://doi.org/10.1039/C8SC05696J	journal	January 2019
Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical Fernández-de-Alba, Carlos; Takahashi, Hiroki; Richard, Alexandre Chemistry - A European Journal, Vol. 21, Issue 12 https://doi.org/10.1002/chem.201404588	journal	February 2015
Targetable Tetrazine‐Based Dynamic Nuclear Polarization Agents for Biological Systems Lim, Byung Joon; Ackermann, Bryce E.; Debelouchina, Galia T. ChemBioChem, Vol. 21, Issue 9 https://doi.org/10.1002/cbic.201900609	journal	January 2020
Dynamic Nuclear Polarization/Solid-State NMR Spectroscopy of Membrane Polypeptides: Free-Radical Optimization for Matrix-Free Lipid Bilayer Samples Salnikov, Evgeniy S.; Abel, Sébastien; Karthikeyan, Ganesan ChemPhysChem, Vol. 18, Issue 15 https://doi.org/10.1002/cphc.201700389	journal	June 2017
De novo design of a transmembrane Zn ²⁺ -transporting four-helix bundle Joh, Nathan H.; Wang, Tuo; Bhate, Manasi P. Science, Vol. 346, Issue 6216 https://doi.org/10.1126/science.1261172	journal	December 2014
Using chemical shift perturbation to characterise ligand binding Williamson, Mike P. Progress in Nuclear Magnetic Resonance Spectroscopy, Vol. 73 https://doi.org/10.1016/j.pnmrs.2013.02.001	journal	August 2013
Polyphenol/Peptide Binding and Precipitation Charlton, Adrian J.; Baxter, Nicola J.; Khan, M. Lokman Journal of Agricultural and Food Chemistry, Vol. 50, Issue 6 https://doi.org/10.1021/jf010897z	journal	March 2002
Determining Cholesterol Binding to Membrane Proteins by Cholesterol ¹³ C Labeling in Yeast and Dynamic Nuclear Polarization NMR Elkins, Matthew R.; Sergeyev, Ivan V.; Hong, Mei Journal of the American Chemical Society, Vol. 140, Issue 45 https://doi.org/10.1021/jacs.8b09658	journal	October 2018
Cholesterol-binding site of the influenza M2 protein in lipid bilayers from solid-state NMR Elkins, Matthew R.; Williams, Jonathan K.; Gelenter, Martin D. Proceedings of the National Academy of Sciences, Vol. 114, Issue 49 https://doi.org/10.1073/pnas.1715127114	journal	November 2017
Influenza Virus A M2 Protein Generates Negative Gaussian Membrane Curvature Necessary for Budding and Scission Schmidt, Nathan W.; Mishra, Abhijit; Wang, Jun Journal of the American Chemical Society, Vol. 135, Issue 37 https://doi.org/10.1021/ja400146z	journal	September 2013
NMR Determination of Protein Partitioning into Membrane Domains with Different Curvatures and Application to the Influenza M2 Peptide Wang, Tuo; Cady, Sarah D.; Hong, Mei Biophysical Journal, Vol. 102, Issue 4 https://doi.org/10.1016/j.bpj.2012.01.010	journal	February 2012
Investigation of the Curvature Induction and Membrane Localization of the Influenza Virus M2 Protein Using Static and Off-Magic-Angle Spinning Solid-State Nuclear Magnetic Resonance of Oriented Bicelles Wang, Tuo; Hong, Mei Biochemistry, Vol. 54, Issue 13 https://doi.org/10.1021/acs.biochem.5b00127	journal	March 2015
Influenza Virus M2 Protein Mediates ESCRT-Independent Membrane Scission Rossman, Jeremy S.; Jing, Xianghong; Leser, George P. Cell, Vol. 142, Issue 6 https://doi.org/10.1016/j.cell.2010.08.029	journal	September 2010
Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes Smith, Adam N.; Twahir, Umar T.; Dubroca, Thierry The Journal of Physical Chemistry B, Vol. 120, Issue 32 https://doi.org/10.1021/acs.jpcb.6b02885	journal	August 2016
Biomolecular DNP-Supported NMR Spectroscopy using Site-Directed Spin Labeling van der Cruijsen, Elwin A. W.; Koers, Eline J.; Sauvée, Claire Chemistry - A European Journal, Vol. 21, Issue 37 https://doi.org/10.1002/chem.201501376	journal	July 2015
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein–protein interfaces Wylie, Benjamin J.; Dzikovski, Boris G.; Pawsey, Shane Journal of Biomolecular NMR, Vol. 61, Issue 3-4 https://doi.org/10.1007/s10858-015-9919-6	journal	April 2015
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR Voinov, Maxim A.; Good, Daryl B.; Ward, Meaghan E. The Journal of Physical Chemistry B, Vol. 119, Issue 32 https://doi.org/10.1021/acs.jpcb.5b05230	journal	July 2015
Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization Viennet, Thibault; Viegas, Aldino; Kuepper, Arne Angewandte Chemie International Edition, Vol. 55, Issue 36 https://doi.org/10.1002/anie.201603205	journal	June 2016
Protein Structure Determination with Paramagnetic Solid-State NMR Spectroscopy Sengupta, Ishita; Nadaud, Philippe S.; Jaroniec, Christopher P. Accounts of Chemical Research, Vol. 46, Issue 9 https://doi.org/10.1021/ar300360q	journal	March 2013
Paramagnet induced signal quenching in MAS–DNP experiments in frozen homogeneous solutions Corzilius, Björn; Andreas, Loren B.; Smith, Albert A. Journal of Magnetic Resonance, Vol. 240 https://doi.org/10.1016/j.jmr.2013.11.013	journal	March 2014
NMR Signal Quenching from Bound Biradical Affinity Reagents in DNP Samples Rogawski, Rivkah; Sergeyev, Ivan V.; Zhang, Yinglu The Journal of Physical Chemistry B, Vol. 121, Issue 48 https://doi.org/10.1021/acs.jpcb.7b08274	journal	November 2017
Relaxation-compensated difference spin diffusion NMR for detecting 13C–13C long-range correlations in proteins and polysaccharides Wang, Tuo; Williams, Jonathan K.; Schmidt-Rohr, Klaus Journal of Biomolecular NMR, Vol. 61, Issue 2 https://doi.org/10.1007/s10858-014-9889-0	journal	December 2014
The Target of β-Expansin EXPB1 in Maize Cell Walls from Binding and Solid-State NMR Studies Wang, Tuo; Chen, Yuning; Tabuchi, Akira Plant Physiology, Vol. 172, Issue 4 https://doi.org/10.1104/pp.16.01311	journal	October 2016
Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR Kaplan, Mohammed; Cukkemane, Abhishek; van Zundert, Gydo C. P. Nature Methods, Vol. 12, Issue 7 https://doi.org/10.1038/nmeth.3406	journal	May 2015
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register Frederick, Kendra K.; Michaelis, Vladimir K.; Caporini, Marc A. Proceedings of the National Academy of Sciences, Vol. 114, Issue 14 https://doi.org/10.1073/pnas.1619051114	journal	March 2017
DNP‐Supported Solid‐State NMR Spectroscopy of Proteins Inside Mammalian Cells Narasimhan, Siddarth; Scherpe, Stephan; Lucini Paioni, Alessandra Angewandte Chemie International Edition, Vol. 58, Issue 37 https://doi.org/10.1002/anie.201903246	journal	July 2019
Observing an Antisense Drug Complex in Intact Human Cells by in‐Cell NMR Spectroscopy Schlagnitweit, Judith; Friebe Sandoz, Sarah; Jaworski, Aleksander ChemBioChem, Vol. 20, Issue 19 https://doi.org/10.1002/cbic.201900297	journal	July 2019
Wall Structure and Wall Loosening. A Look Backwards and Forwards: Fig. 1. Cosgrove, Daniel J. Plant Physiology, Vol. 125, Issue 1 https://doi.org/10.1104/pp.125.1.131	journal	January 2001
Hidden Killers: Human Fungal Infections Brown, G. D.; Denning, D. W.; Gow, N. A. R. Science Translational Medicine, Vol. 4, Issue 165 https://doi.org/10.1126/scitranslmed.3004404	journal	December 2012
From the surface to the inner layer of the fungal cell wall Fontaine, T.; Mouyna, I.; Hartland, R. P. Biochemical Society Transactions, Vol. 25, Issue 1 https://doi.org/10.1042/bst0250194	journal	February 1997
Spectral Snapshots of Bacterial Cell-Wall Composition and the Influence of Antibiotics by Whole-Cell NMR Nygaard, Rie; Romaniuk, Joseph A. H.; Rice, David M. Biophysical Journal, Vol. 108, Issue 6 https://doi.org/10.1016/j.bpj.2015.01.037	journal	March 2015
Whole cell solid-state NMR study of Chlamydomonas reinhardtii microalgae Arnold, Alexandre A.; Bourgouin, Jean-Philippe; Genard, Bertrand Journal of Biomolecular NMR, Vol. 70, Issue 2 https://doi.org/10.1007/s10858-018-0164-7	journal	January 2018
Solid-state NMR Reveals the Carbon-based Molecular Architecture of Cryptococcus neoformans Fungal Eumelanins in the Cell Wall Chatterjee, Subhasish; Prados-Rosales, Rafael; Itin, Boris Journal of Biological Chemistry, Vol. 290, Issue 22 https://doi.org/10.1074/jbc.M114.618389	journal	March 2015
Molecular architecture of softwood revealed by solid-state NMR Terrett, Oliver M.; Lyczakowski, Jan J.; Yu, Li Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12979-9	journal	October 2019
Folding of xylan onto cellulose fibrils in plant cell walls revealed by solid-state NMR Simmons, Thomas J.; Mortimer, Jenny C.; Bernardinelli, Oigres D. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms13902	journal	December 2016
Structure and Dynamics of Brachypodium Primary Cell Wall Polysaccharides from Two-Dimensional ¹³ C Solid-State Nuclear Magnetic Resonance Spectroscopy Wang, Tuo; Salazar, Andre; Zabotina, Olga A. Biochemistry, Vol. 53, Issue 17 https://doi.org/10.1021/bi500231b	journal	April 2014
Cellulose Structural Polymorphism in Plant Primary Cell Walls Investigated by High-Field 2D Solid-State NMR Spectroscopy and Density Functional Theory Calculations Wang, Tuo; Yang, Hui; Kubicki, James D. Biomacromolecules, Vol. 17, Issue 6 https://doi.org/10.1021/acs.biomac.6b00441	journal	May 2016
Gradients in Wall Mechanics and Polysaccharides along Growing Inflorescence Stems Phyo, Pyae; Wang, Tuo; Kiemle, Sarah N. Plant Physiology, Vol. 175, Issue 4 https://doi.org/10.1104/pp.17.01270	journal	October 2017
Effects of Pectin Molecular Weight Changes on the Structure, Dynamics, and Polysaccharide Interactions of Primary Cell Walls of Arabidopsis thaliana : Insights from Solid-State NMR Phyo, Pyae; Wang, Tuo; Xiao, Chaowen Biomacromolecules, Vol. 18, Issue 9 https://doi.org/10.1021/acs.biomac.7b00888	journal	August 2017
Phosphoethanolamine cellulose: A naturally produced chemically modified cellulose Thongsomboon, Wiriya; Serra, Diego O.; Possling, Alexandra Science, Vol. 359, Issue 6373 https://doi.org/10.1126/science.aao4096	journal	January 2018
Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR Romaniuk, Joseph A. H.; Cegelski, Lynette Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 370, Issue 1679 https://doi.org/10.1098/rstb.2015.0024	journal	October 2015
Solid-state NMR of plant and fungal cell walls: A critical review Zhao, Wancheng; Fernando, Liyanage D.; Kirui, Alex Solid State Nuclear Magnetic Resonance, Vol. 107 https://doi.org/10.1016/j.ssnmr.2020.101660	journal	June 2020
Cellulose-Pectin Spatial Contacts Are Inherent to Never-Dried Arabidopsis Primary Cell Walls: Evidence from Solid-State Nuclear Magnetic Resonance Wang, Tuo; Park, Yong Bum; Cosgrove, Daniel J. Plant Physiology, Vol. 168, Issue 3 https://doi.org/10.1104/pp.15.00665	journal	June 2015
Multidimensional solid-state NMR spectroscopy of plant cell walls Wang, Tuo; Phyo, Pyae; Hong, Mei Solid State Nuclear Magnetic Resonance, Vol. 78 https://doi.org/10.1016/j.ssnmr.2016.08.001	journal	September 2016
Atomic resolution of cotton cellulose structure enabled by dynamic nuclear polarization solid-state NMR Kirui, Alex; Ling, Zhe; Kang, Xue Cellulose, Vol. 26, Issue 1 https://doi.org/10.1007/s10570-018-2095-6	journal	November 2018
Rapid Natural-Abundance 2D ¹³ C- ¹³ C Correlation Spectroscopy Using Dynamic Nuclear Polarization Enhanced Solid-State NMR and Matrix-Free Sample Preparation Takahashi, Hiroki; Lee, Daniel; Dubois, Lionel Angewandte Chemie International Edition, Vol. 51, Issue 47 https://doi.org/10.1002/anie.201206102	journal	October 2012
Matrix-free dynamic nuclear polarization enables solid-state NMR 13C–13C correlation spectroscopy of proteins at natural isotopic abundance Takahashi, Hiroki; Hediger, Sabine; De Paëpe, Gaël Chemical Communications, Vol. 49, Issue 82 https://doi.org/10.1039/c3cc45195j	journal	January 2013
Solid-State NMR on Bacterial Cells: Selective Cell Wall Signal Enhancement and Resolution Improvement using Dynamic Nuclear Polarization Takahashi, Hiroki; Ayala, Isabel; Bardet, Michel Journal of the American Chemical Society, Vol. 135, Issue 13 https://doi.org/10.1021/ja312501d	journal	February 2013
Two-dimensional magic angle spinning NMR investigation of naturally occurring chitins: Precise1H and13C resonance assignment of ?- and ?-chitin Kono, Hiroyuki Biopolymers, Vol. 75, Issue 3 https://doi.org/10.1002/bip.20124	journal	January 2004
Hydrogen Bonding Structure and Stability of?-Chitin Studied by13C Solid-State NMR Kameda, Tsunenori; Miyazawa, Mitsuhiro; Ono, Hiroshi Macromolecular Bioscience, Vol. 5, Issue 2 https://doi.org/10.1002/mabi.200400142	journal	February 2005
Revisit of α-Chitin Crystal Structure Using High Resolution X-ray Diffraction Data Sikorski, Pawel; Hori, Ritsuko; Wada, Masahisa Biomacromolecules, Vol. 10, Issue 5 https://doi.org/10.1021/bm801251e	journal	May 2009
Exhaustive crystal structure search and crystal modeling of β-chitin Yui, Toshifumi; Taki, Naoto; Sugiyama, Junji International Journal of Biological Macromolecules, Vol. 40, Issue 4 https://doi.org/10.1016/j.ijbiomac.2006.08.017	journal	March 2007
¹⁵ N− ¹⁵ N Proton Assisted Recoupling in Magic Angle Spinning NMR Lewandowski, Józef R.; Paëpe, Gaël De; Eddy, Matthew T. Journal of the American Chemical Society, Vol. 131, Issue 16 https://doi.org/10.1021/ja806578y	journal	April 2009
Proton assisted recoupling and protein structure determination De Paëpe, Gaël; Lewandowski, Józef R.; Loquet, Antoine The Journal of Chemical Physics, Vol. 129, Issue 24 https://doi.org/10.1063/1.3036928	journal	December 2008
Proton-Assisted Recoupling (PAR) in Peptides and Proteins Donovan, Kevin J.; Jain, Sheetal K.; Silvers, Robert The Journal of Physical Chemistry B, Vol. 121, Issue 48 https://doi.org/10.1021/acs.jpcb.7b08934	journal	November 2017
A quasi-optical and corrugated waveguide microwave transmission system for simultaneous dynamic nuclear polarization NMR on two separate 14.1 T spectrometers Dubroca, Thierry; Smith, Adam N.; Pike, Kevin J. Journal of Magnetic Resonance, Vol. 289 https://doi.org/10.1016/j.jmr.2018.01.015	journal	April 2018
Topology of Pretreated Wood Fibers Using Dynamic Nuclear Polarization Viger-Gravel, Jasmine; Lan, Wu; Pinon, Arthur C. The Journal of Physical Chemistry C, Vol. 123, Issue 50 https://doi.org/10.1021/acs.jpcc.9b09272	journal	November 2019
Atomic-Level Structure Characterization of Biomass Pre- and Post-Lignin Treatment by Dynamic Nuclear Polarization-Enhanced Solid-State NMR Perras, Frédéric A.; Luo, Hao; Zhang, Ximing The Journal of Physical Chemistry A, Vol. 121, Issue 3 https://doi.org/10.1021/acs.jpca.6b11121	journal	January 2017
Dynamic regulation of HIV-1 capsid interaction with the restriction factor TRIM5α identified by magic-angle spinning NMR and molecular dynamics simulations Quinn, Caitlin M.; Wang, Mingzhang; Fritz, Matthew P. Proceedings of the National Academy of Sciences, Vol. 115, Issue 45 https://doi.org/10.1073/pnas.1800796115	journal	October 2018
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies Gupta, Rupal; Lu, Manman; Hou, Guangjin The Journal of Physical Chemistry B, Vol. 120, Issue 2 https://doi.org/10.1021/acs.jpcb.5b12134	journal	January 2016
Chemical Shifts for the Unusual DNA Structure in Pf1 Bacteriophage from Dynamic-Nuclear-Polarization-Enhanced Solid-State NMR Spectroscopy Sergeyev, Ivan V.; Day, Loren A.; Goldbourt, Amir Journal of the American Chemical Society, Vol. 133, Issue 50 https://doi.org/10.1021/ja2043062	journal	December 2011
Structural description of surfaces and interfaces in biominerals by DNP SENS Azaïs, Thierry; Von Euw, Stanislas; Ajili, Widad Solid State Nuclear Magnetic Resonance, Vol. 102 https://doi.org/10.1016/j.ssnmr.2019.06.001	journal	October 2019
Detection of nucleic acids and other low abundance components in native bone and osteosarcoma extracellular matrix by isotope enrichment and DNP-enhanced NMR Goldberga, Ieva; Li, Rui; Chow, Wing Ying RSC Advances, Vol. 9, Issue 46 https://doi.org/10.1039/C9RA03198G	journal	January 2019
Essential but sparse collagen hydroxylysyl post-translational modifications detected by DNP NMR Chow, Wing Ying; Li, Rui; Goldberga, Ieva Chemical Communications, Vol. 54, Issue 89 https://doi.org/10.1039/C8CC04960B	journal	January 2018
¹⁹ F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies Lu, Manman; Wang, Mingzhang; Sergeyev, Ivan V. Journal of the American Chemical Society, Vol. 141, Issue 14 https://doi.org/10.1021/jacs.8b09216	journal	March 2019
Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning Jaudzems, Kristaps; Bertarello, Andrea; Chaudhari, Sachin R. Angewandte Chemie International Edition, Vol. 57, Issue 25 https://doi.org/10.1002/anie.201801016	journal	April 2018
Natural Isotopic Abundance ¹³ C and ¹⁵ N Multidimensional Solid-State NMR Enabled by Dynamic Nuclear Polarization Smith, Adam N.; Märker, Katharina; Hediger, Sabine The Journal of Physical Chemistry Letters, Vol. 10, Issue 16 https://doi.org/10.1021/acs.jpclett.8b03874	journal	July 2019
Structural Fingerprinting of Protein Aggregates by Dynamic Nuclear Polarization-Enhanced Solid-State NMR at Natural Isotopic Abundance Smith, Adam N.; Märker, Katharina; Piretra, Talia Journal of the American Chemical Society, Vol. 140, Issue 44 https://doi.org/10.1021/jacs.8b09002	journal	October 2018
Welcoming natural isotopic abundance in solid-state NMR: probing π-stacking and supramolecular structure of organic nanoassemblies using DNP Märker, Katharina; Paul, Subhradip; Fernández-de-Alba, Carlos Chemical Science, Vol. 8, Issue 2 https://doi.org/10.1039/C6SC02709A	journal	January 2017
CCMRD: a solid-state NMR database for complex carbohydrates Kang, Xue; Zhao, Wancheng; Dickwella Widanage, Malitha C. Journal of Biomolecular NMR, Vol. 74, Issue 4-5 https://doi.org/10.1007/s10858-020-00304-2	journal	March 2020

Similar Records

Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy

Journal Article · Thu May 07 00:00:00 EDT 2020 · Biochemical Society Transactions · OSTI ID:1878627

Chakraborty, Arnab; Deligey, Fabien; Quach, Jenny; +3 more

Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants

Journal Article · Wed Dec 08 00:00:00 EST 2021 · Chemical Reviews · OSTI ID:1878627

Ghassemi, Nader; Poulhazan, Alexandre; Deligey, Fabien; +3 more

Current limitations of solid-state NMR in carbohydrate and cell wall research

Journal Article · Sat Jul 02 00:00:00 EDT 2022 · Journal of Magnetic Resonance · OSTI ID:1878627

Zhao, Wancheng; Deligey, Fabien; Chandra Shekar, S.; +2 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
36 MATERIALS SCIENCE
cell wall
dynamic nuclear polarization
membrane proteins
pathogenic fungi
polysaccharides
solid-state NMR

Title: Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy

Citation Formats

References (98)

Similar Records

Related Subjects