Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering

Lee, Sang Jin; Kim, Tae Wu; Kim, Jong Goo; Yang, Cheolhee; Yun, So Ri; Kim, Changin; Ren, Zhong; Kumarapperuma, Indika; Kuk, Jane; Moffat, Keith; Yang, Xiaojing; Ihee, Hyotcherl

doi:10.1126/sciadv.abm6278

Light-induced protein structural dynamics in bacteriophytochrome revealed by time-resolved x-ray solution scattering

Journal Article · Fri May 27 00:00:00 EDT 2022 · Science Advances

DOI:https://doi.org/10.1126/sciadv.abm6278· OSTI ID:1877219

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Korea Advanced Inst. Science and Technology (KAIST), Daejeon (Korea, Republic of); Institute for Basic Science (IBS) (Korea, Republic of)
Mokpo National University (Korea, Republic of)
Univ. of Illinois, Chicago, IL (United States)
Univ. of Chicago, IL (United States)

Bacteriophytochromes (BphPs) are photoreceptors that regulate a wide range of biological mechanisms via red light–absorbing (Pr)–to–far-red light–absorbing (Pfr) reversible photoconversion. The structural dynamics underlying Pfr-to-Pr photoconversion in a liquid solution phase are not well understood. We used time-resolved x-ray solution scattering (TRXSS) to capture light-induced structural transitions in the bathy BphP photosensory module of Pseudomonas aeruginosa. Kinetic analysis of the TRXSS data identifies three distinct structural species, which are attributed to lumi-F, meta-F, and Pr, connected by time constants of 95 μs and 21 ms. Structural analysis based on molecular dynamics simulations shows that the light activation of PaBphP accompanies quaternary structural rearrangements from an “II”-framed close form of the Pfr state to an “O”-framed open form of the Pr state in terms of the helical backbones. This study provides mechanistic insights into how modular signaling proteins such as BphPs transmit structural signals over long distances and regulate their downstream biological responses.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1877219

Journal Information:: Science Advances, Journal Name: Science Advances Journal Issue: 21 Vol. 8; ISSN 2375-2548

Publisher:: AAASCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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