Spectroscopy-based isotopic (δ13C) analysis for high spatial resolution of carbon exchange in the rhizosphere

Moran, James J.; Linley, Timothy J.; Makarem, Camille N.; Kelly, James F.; Wilcox Freeburg, Eric D.; Cleary, Daniel M.; Alexander, M. Lizabeth; Kriesel, Jason M.

doi:10.1016/j.rhisph.2022.100564

Spectroscopy-based isotopic (δ¹³C) analysis for high spatial resolution of carbon exchange in the rhizosphere

Journal Article · Thu Jun 23 00:00:00 EDT 2022 · Rhizosphere

DOI:https://doi.org/10.1016/j.rhisph.2022.100564· OSTI ID:1876187

^[1]; Linley, Timothy J. ^[2]; Makarem, Camille N. ^[3]; Kelly, James F. ^[3]; Wilcox Freeburg, Eric D. ^[2]; Cleary, Daniel M. ^[2]; Alexander, M. Lizabeth ^[2]; Kriesel, Jason M. ^[3]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Washington State Univ., Pullman, WA (United States); Pacific Northwest National Laboratory
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Opto-Knowledge Systems, Inc., Torrance, CA (United States)

The rhizosphere is a highly dynamic zone bridging plant roots with needed nutrient resources in soil. While the rhizosphere may be small, it has a disproportionally large impact on plant success and biomass production. A suite of rhizosphere-hosted microbial and geochemical interactions facilitate nutrient acquisition by plant roots, and, in turn, the roots stimulate these processes by supplying organic carbon into the rhizosphere. The small physical dimensions of the rhizosphere, however, can constrain efforts to elucidate key carbon exchange processes and their spatial extent and localization. We present a method for spatially resolved δ¹³C analysis of rhizosphere samples by coupling laser ablation (LA) sampling with isotopic analysis using capillary absorption spectroscopy (CAS) which differs from conventional mass spectrometer (MS) approaches. The CAS system has high sensitivity (requires fewer nanomoles of CO₂ per analysis) than comparable MS systems, which enables reduced sample size requirements to thereby improve spatial resolution (from 25 μm to as low as a projected 5 μm spatial resolution). We demonstrate the utility of CAS using rhizosphere samples from switchgrass plants exposed to ¹³CO₂. As a result, this technique will provide a capability for tracking the extent and spatial distribution of root exudate into the rhizosphere at highly detailed spatial scales.

View Accepted Manuscript (DOE)

Research Organization:: Opto-Knowledge Systems, Inc., Torrance, CA (United States); Univ. of Wisconsin, Madison, WI (United States). Great Lakes Bioenergy Research Center

Sponsoring Organization:: National Science Foundation; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830; FC02-07ER64494; SC0018409; SC0018488

OSTI ID:: 1876187

Journal Information:: Rhizosphere, Journal Name: Rhizosphere Vol. 23; ISSN 2452-2198

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Spectroscopy-based isotopic (δ13C) analysis for high spatial resolution of carbon exchange in the rhizosphere

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Spectroscopy-based isotopic (δ¹³C) analysis for high spatial resolution of carbon exchange in the rhizosphere