Spatially tracking carbon through the root-rhizosphere-soil system using laser ablation-IRMS

Denis, Elizabeth H.; Ilhardt, Peter D.; Tucker, Abigail E.; Huggett, Nicholas L.; Rosnow, Joshua J.; Moran, James J.

doi:10.1002/jpln.201800301

Spatially tracking carbon through the root-rhizosphere-soil system using laser ablation-IRMS

Journal Article · Thu Mar 28 00:00:00 EDT 2019 · Journal of Plant Nutrition and Soil Science (Online)

DOI:https://doi.org/10.1002/jpln.201800301· OSTI ID:1499134

Denis, Elizabeth H. ^[1]; Ilhardt, Peter D. ^[1]; Tucker, Abigail E. ^[1]; Huggett, Nicholas L. ^[1]; Rosnow, Joshua J. ^[1]; Moran, James J. ^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The intimate relationships between plant roots, rhizosphere, and soil are fostered by the release of organic compounds from the plant into soil through various forms of rhizodeposition and the simultaneous harvestingof inorganic nutrients from the soil to the plant. Here we present a method to spatially track and map the migration of plant-derived carbon (C) through roots into the rhizosphere and surrounding soil using laser ablation-isotope ratio mass spectrometry (LA-IRMS). Weused switchgrass microcosms containing soil from field plots at the Kellogg Biological Station (Hickory Corners, Michigan, USA), which have been cropped with switchgrass since 2008. We used a ¹³CO₂ tracerto isotopically label switchgrass plants for two diel cycles and tracked subsequent movement of labeled C using the spatially specific (<100 µm resolution) δ¹³C analysis enabled by LA-IRMS.This approach permitted assessment of variable C flow through different roots and enabled mapping of spatial variability of C allocation to the rhizosphere. Highly ¹³C-enriched C (consistent with production during the ¹³CO₂ application period) extended ~0.5 – 1 mm from the root into the soil, suggesting that the majority of recent plant-derived C was within this distance of the root after 48 hours. Tracking the physical extent of root exudation into the rhizosphere can help evaluate the localization of plant-microbe interactions in highly variable subsurface environments,and the use of the isotopic label can differentiate freshly fixed C (presumably from root exudates) from other types of subsurface C (e.g., plant necromass and microbial turnover). The LA-IRMS technique mayalso serve as a valuable screening technique to identify areas of high activity for additional microbial or geochemicalassays.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1499134

Alternate ID(s):: OSTI ID: 1503718
OSTI ID: 1567258

Report Number(s):: PNNL-SA--135618; PNNL-SA-135618

Journal Information:: Journal of Plant Nutrition and Soil Science (Online), Journal Name: Journal of Plant Nutrition and Soil Science (Online) Journal Issue: 3 Vol. 182; ISSN 1522-2624

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

References (40)

Carbon input by plants into the soil. Review Kuzyakov, Yakov; Domanski, Grzegorz Journal of Plant Nutrition and Soil Science, Vol. 163, Issue 4 https://doi.org/10.1002/1522-2624(200008)163:4<421::AID-JPLN421>3.0.CO;2-R	journal	August 2000
Spatial distribution of root exudates of five plant species as assessed by14C labeling Sauer, Daniela; Kuzyakov, Yakov; Stahr, Karl Journal of Plant Nutrition and Soil Science, Vol. 169, Issue 3 https://doi.org/10.1002/jpln.200621974	journal	June 2006
Photoassimilate allocation and dynamics of hotspots in roots visualized by 14C phosphor imaging Pausch, Johanna; Kuzyakov, Yakov Journal of Plant Nutrition and Soil Science, Vol. 174, Issue 1 https://doi.org/10.1002/jpln.200900271	journal	February 2011
Laser ablation isotope ratio mass spectrometry for enhanced sensitivity and spatial resolution in stable isotope analysis: LA-IRMS for enhanced sensitivity in stable isotope analysis Moran, James J.; Newburn, Matt K.; Alexander, M. Lizabeth Rapid Communications in Mass Spectrometry, Vol. 25, Issue 9 https://doi.org/10.1002/rcm.4985	journal	April 2011
Determination of rhizosphere13C pulse signals in soil thin sections by laser ablation isotope ratio mass spectrometry Bruneau, Patricia M. C.; Ostle, Nick; Davidson, Donald A. Rapid Communications in Mass Spectrometry, Vol. 16, Issue 23 https://doi.org/10.1002/rcm.740	journal	January 2002
Stable carbon isotope analyses of nanogram quantities of particulate organic carbon (pollen) with laser ablation nano combustion gas chromatography/isotope ratio mass spectrometry van Roij, Linda; Sluijs, Appy; Laks, Jelmer J. Rapid Communications in Mass Spectrometry, Vol. 31, Issue 1 https://doi.org/10.1002/rcm.7769	journal	November 2016
Influence of mechanical impedance on root exudation of maize seedlings at two development stages Boeuf-Tremblay, V.; Plantureux, S.; Guckert, A. Plant and Soil, Vol. 172, Issue 2 https://doi.org/10.1007/BF00011330	journal	January 1995
Rhizosphere: biophysics, biogeochemistry and ecological relevance Hinsinger, Philippe; Bengough, A. Glyn; Vetterlein, Doris Plant and Soil, Vol. 321, Issue 1-2 https://doi.org/10.1007/s11104-008-9885-9	journal	January 2009
Plant-microbe-soil interactions in the rhizosphere: an evolutionary perspective Lambers, Hans; Mougel, Christophe; Jaillard, Benoît Plant and Soil, Vol. 321, Issue 1-2 https://doi.org/10.1007/s11104-009-0042-x	journal	June 2009
Carbon flow in the rhizosphere: carbon trading at the soil–root interface Jones, D. L.; Nguyen, C.; Finlay, R. D. Plant and Soil, Vol. 321, Issue 1-2 https://doi.org/10.1007/s11104-009-9925-0	journal	February 2009
Intra-annual variability of wood formation and δ13C in tree-rings at Hyytiälä, Finland Soudant, Alex; Loader, Neil J.; Bäck, Janna Agricultural and Forest Meteorology, Vol. 224 https://doi.org/10.1016/j.agrformet.2016.04.015	journal	August 2016
Inter-annual carbon isotope analysis of tree-rings by laser ablation Loader, N. J.; McCarroll, D.; Barker, S. Chemical Geology, Vol. 466 https://doi.org/10.1016/j.chemgeo.2017.06.021	journal	September 2017
Micromapping of microbial hotspots and biofilms from different crops using digital image mosaics of soil thin sections Gutiérrez Castorena, Edgar Vladimir; Gutiérrez-Castorena, Ma. del Carmen; González Vargas, Tania Geoderma, Vol. 279 https://doi.org/10.1016/j.geoderma.2016.05.017	journal	October 2016
Lipidomics in situ: Insights into plant lipid metabolism from high resolution spatial maps of metabolites Horn, Patrick J.; Chapman, Kent D. Progress in Lipid Research, Vol. 54 https://doi.org/10.1016/j.plipres.2014.01.003	journal	April 2014
Laser Ablation Molecular Isotopic Spectrometry Russo, Richard E.; Bol'shakov, Alexander A.; Mao, Xianglei Spectrochimica Acta Part B: Atomic Spectroscopy, Vol. 66, Issue 2 https://doi.org/10.1016/j.sab.2011.01.007	journal	February 2011
Spatial heterogeneity in the relocation of added 13C within the structure of an upland grassland soil Grieve, Ian C.; Davidson, Donald A.; Ostle, Nicholas J. Soil Biology and Biochemistry, Vol. 38, Issue 2 https://doi.org/10.1016/j.soilbio.2005.04.035	journal	February 2006
Rhizosphere interactions between microorganisms and plants govern iron and phosphorus acquisition along the root axis – model and research methods Marschner, Petra; Crowley, David; Rengel, Zed Soil Biology and Biochemistry, Vol. 43, Issue 5 https://doi.org/10.1016/j.soilbio.2011.01.005	journal	May 2011
Microbial hotspots and hot moments in soil: Concept & review Kuzyakov, Yakov; Blagodatskaya, Evgenia Soil Biology and Biochemistry, Vol. 83 https://doi.org/10.1016/j.soilbio.2015.01.025	journal	April 2015
High-resolution elemental mapping of the root-rhizosphere-soil continuum using laser-induced breakdown spectroscopy (LIBS) Ilhardt, Peter D.; Nuñez, Jamie R.; Denis, Elizabeth H. Soil Biology and Biochemistry, Vol. 131 https://doi.org/10.1016/j.soilbio.2018.12.029	journal	April 2019
Carbon Isotope Separation and Molecular Formation in Laser-Induced Plasmas by Laser Ablation Molecular Isotopic Spectrometry Dong, Meirong; Mao, Xianglei; Gonzalez, Jhanis J. Analytical Chemistry, Vol. 85, Issue 5 https://doi.org/10.1021/ac303524d	journal	February 2013
Hotspots of soil N2O emission enhanced through water absorption by plant residue Kravchenko, A. N.; Toosi, E. R.; Guber, A. K. Nature Geoscience, Vol. 10, Issue 7 https://doi.org/10.1038/ngeo2963	journal	June 2017
Going back to the roots: the microbial ecology of the rhizosphere Philippot, Laurent; Raaijmakers, Jos M.; Lemanceau, Philippe Nature Reviews Microbiology, Vol. 11, Issue 11 https://doi.org/10.1038/nrmicro3109	journal	September 2013
Spatially-Resolved Proteomics: Rapid Quantitative Analysis of Laser Capture Microdissected Alveolar Tissue Samples Clair, Geremy; Piehowski, Paul D.; Nicola, Teodora Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep39223	journal	December 2016
Laser ablation molecular isotopic spectrometry (LAMIS): current state of the art Bol'shakov, Alexander A.; Mao, Xianglei; González, Jhanis J. Journal of Analytical Atomic Spectrometry, Vol. 31, Issue 1 https://doi.org/10.1039/C5JA00310E	journal	January 2016
The control of carbon acquisition by roots: REVIEW Control of carbon acquisition by roots Farrar, J. F.; Jones, D. L. New Phytologist, Vol. 147, Issue 1 https://doi.org/10.1046/j.1469-8137.2000.00688.x	journal	July 2000
Root hairs increase rhizosphere extension and carbon input to soil Holz, Maire; Zarebanadkouki, Mohsen; Kuzyakov, Yakov Annals of Botany, Vol. 121, Issue 1 https://doi.org/10.1093/aob/mcx127	journal	December 2017
Laser ablation-combustion-GC-IRMS--a new method for online analysis of intra-annual variation of 13C in tree rings Schulze, B.; Wirth, C.; Linke, P. Tree Physiology, Vol. 24, Issue 11 https://doi.org/10.1093/treephys/24.11.1193	journal	November 2004
In Situ Mapping of Nutrient Uptake in the Rhizosphere Using Nanoscale Secondary Ion Mass Spectrometry Clode, Peta L.; Kilburn, Matt R.; Jones, David L. Plant Physiology, Vol. 151, Issue 4 https://doi.org/10.1104/pp.109.141499	journal	October 2009
Spatially tracking ¹³ C-labelled substrate (bicarbonate) accumulation in microbial communities using laser ablation isotope ratio mass spectrometry : Spatial tracking of substrate into microbial mat Moran, James J.; Doll, Charles G.; Bernstein, Hans C. Environmental Microbiology Reports, Vol. 6, Issue 6 https://doi.org/10.1111/1758-2229.12211	journal	October 2014
Rhizosphere processes are quantitatively important components of terrestrial carbon and nutrient cycles Finzi, Adrien C.; Abramoff, Rose Z.; Spiller, Kimberly S. Global Change Biology, Vol. 21, Issue 5 https://doi.org/10.1111/gcb.12816	journal	January 2015
Plant and mycorrhizal regulation of rhizodeposition Jones, David L.; Hodge, Angela; Kuzyakov, Yakov New Phytologist, Vol. 163, Issue 3 https://doi.org/10.1111/j.1469-8137.2004.01130.x	journal	September 2004
Rhizosphere geometry and heterogeneity arising from root-mediated physical and chemical processes: Research review Hinsinger, Philippe; Gobran, George R.; Gregory, Peter J. New Phytologist, Vol. 168, Issue 2 https://doi.org/10.1111/j.1469-8137.2005.01512.x	journal	August 2005
Are root exudates more important than other sources of rhizodeposits in structuring rhizosphere bacterial communities?: Root exudates and rhizosphere bacteria Dennis, Paul G.; Miller, Anthony J.; Hirsch, Penny R. FEMS Microbiology Ecology, Vol. 72, Issue 3 https://doi.org/10.1111/j.1574-6941.2010.00860.x	journal	March 2010
Exploring the transfer of recent plant photosynthates to soil microbes: mycorrhizal pathway vs direct root exudation Kaiser, Christina; Kilburn, Matt R.; Clode, Peta L. New Phytologist, Vol. 205, Issue 4 https://doi.org/10.1111/nph.13138	journal	November 2014
Allocation, stress tolerance and carbon transport in plants: how does phloem physiology affect plant ecology?: Phloem ecophysiology Savage, Jessica A.; Clearwater, Michael J.; Haines, Dustin F. Plant, Cell & Environment, Vol. 39, Issue 4 https://doi.org/10.1111/pce.12602	journal	September 2015
Soil Resources, Microbial Activity, and Primary Production Across an Agricultural Ecosystem Robertson, G. Philip; Klingensmith, Katherine M.; Klug, Michael J. Ecological Applications, Vol. 7, Issue 1 https://doi.org/10.1890/1051-0761(1997)007[0158:SRMAAP]2.0.CO;2	journal	February 1997
Linking 3D Soil Structure and Plant-Microbe-Soil Carbon Transfer in the Rhizosphere Vidal, Alix; Hirte, Juliane; Bender, S. Franz Frontiers in Environmental Science, Vol. 6 https://doi.org/10.3389/fenvs.2018.00009	journal	February 2018
Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI Bartels, Benjamin; Svatoš, Aleš Frontiers in Plant Science, Vol. 6 https://doi.org/10.3389/fpls.2015.00471	journal	July 2015
Stage-dependent border cell and carbon flow from roots to rhizosphere Odell, Ryan E.; Dumlao, Matthew R.; Samar, Danial American Journal of Botany, Vol. 95, Issue 4 https://doi.org/10.3732/ajb.95.4.441	journal	April 2008
Switchgrass as a biofuels feedstock in the USA Sanderson, M. A.; Adler, P. R.; Boateng, A. A. Canadian Journal of Plant Science, Vol. 86, Issue Special Issue https://doi.org/10.4141/P06-136	journal	December 2006

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Spatially tracking carbon through the root-rhizosphere-soil system using laser ablation-IRMS

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