A Reduced F 420 -Dependent Nitrite Reductase in an Anaerobic Methanotrophic Archaeon

Heryakusuma, Christian; Susanti, Dwi; Yu, Hang; Li, Zhou; Purwantini, Endang; Hettich, Robert L.; Orphan, Victoria J.; Mukhopadhyay, Biswarup; Maupin-Furlow, ed., Julie A.

doi:10.1128/jb.00078-22

Title: A Reduced F ₄₂₀ -Dependent Nitrite Reductase in an Anaerobic Methanotrophic Archaeon

Journal Article · Tue Jul 19 00:00:00 EDT 2022 · Journal of Bacteriology

DOI:https://doi.org/10.1128/jb.00078-22· OSTI ID:1874394

Heryakusuma, Christian ^[1]; Susanti, Dwi ^[2];

^[3]; Li, Zhou ^[4]; Purwantini, Endang ^[2]; Hettich, Robert L. ^[4]; Orphan, Victoria J. ^[3]; Mukhopadhyay, Biswarup ^[5]; Maupin-Furlow, ed., Julie A.

Genetics, Bioinformatics, and Computational Biology Ph.D. Program, Virginia Tech, Blacksburg, Virginia, USA, Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, USA
Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, USA
Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California, USA
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA
Department of Biochemistry, Virginia Tech, Blacksburg, Virginia, USA, Virginia Tech Carilion School of Medicine, Virginia Tech, Blacksburg, Virginia, USA

Anaerobic methanotrophic archaea (ANME), which oxidize methane in marine sediments through syntrophic associations with sulfate-reducing bacteria, carry homologs of coenzyme F₄₂₀-dependent sulfite reductase (Fsr) of Methanocaldococcus jannaschii, a hyperthermophilic methanogen from deep-sea hydrothermal vents. M. jannaschii Fsr (MjFsr) and ANME-Fsr belong to two phylogenetically distinct groups, FsrI and FsrII, respectively. MjFsrI reduces sulfite to sulfide with reduced F₄₂₀ (F₄₂₀H₂), protecting methyl coenzyme M reductase (Mcr), an essential enzyme for methanogens, from sulfite inhibition. However, the function of FsrIIs in ANME, which also rely on Mcr and live in sulfidic environments, is unknown. We have determined the catalytic properties of FsrII from a member of ANME-2c. Since ANME remain to be isolated, we expressed ANME2c-FsrII in a closely related methanogen, Methanosarcina acetivorans. Purified recombinant FsrII contained siroheme, indicating that the methanogen, which lacks a native sulfite reductase, produced this coenzyme. Unexpectedly, FsrII could not reduce sulfite or thiosulfate with F₄₂₀H₂. Instead, it acted as an F₄₂₀H₂-dependent nitrite reductase (FNiR) with physiologically relevant Km values (nitrite, 5 μM; F₄₂₀H₂, 14 μM). From kinetic, thermodynamic, and structural analyses, we hypothesize that in FNiR, F₄₂₀H₂-derived electrons are delivered at the oxyanion reduction site at a redox potential that is suitable for reducing nitrite (E⁰' [standard potential], +440 mV) but not sulfite (E⁰', –116 mV). These findings and the known nitrite sensitivity of Mcr suggest that FNiR may protect nondenitrifying ANME from nitrite toxicity. Remarkably, by reorganizing the reductant processing system, Fsr transforms two analogous oxyanions in two distinct archaeal lineages with different physiologies and ecologies.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Aeronautics and Space Administration (NASA); Virginia Tech Agricultural Experiment Station Hatch Program; Gordon and Betty Moore Foundation

Grant/Contract Number:: SC0020373; AC05-00OR22725; NNX13AI05G; VA-160021; 9324

OSTI ID:: 1874394

Alternate ID(s):: OSTI ID: 1883771

Journal Information:: Journal of Bacteriology, Journal Name: Journal of Bacteriology Vol. 204 Journal Issue: 7; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Siro(haem)amide in Allochromatium vinosum and relevance of DsrL and DsrN, a homolog of cobyrinic acid a,c -diamide synthase, for sulphur oxidation LÃ¼bbe, Yvonne J.; Youn, Hyung-Sun; Timkovich, Russell FEMS Microbiology Letters, Vol. 261, Issue 2 https://doi.org/10.1111/j.1574-6968.2006.00343.x	journal	August 2006
Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum. Jacobson, F. S.; Daniels, L.; Fox, J. A. Journal of Biological Chemistry, Vol. 257, Issue 7 https://doi.org/10.1016/S0021-9258(18)34788-4	journal	April 1982
Structure of the Dissimilatory Sulfite Reductase from the Hyperthermophilic Archaeon Archaeoglobus fulgidus Schiffer, Alexander; Parey, Kristian; Warkentin, Eberhard Journal of Molecular Biology, Vol. 379, Issue 5 https://doi.org/10.1016/j.jmb.2008.04.027	journal	June 2008
Cloning, expression, and nucleotide sequence of the formate dehydrogenase genes from Methanobacterium formicicum. Shuber, A. P.; Orr, E. C.; Recny, M. A. Journal of Biological Chemistry, Vol. 261, Issue 28 https://doi.org/10.1016/S0021-9258(18)69253-1	journal	October 1986
A Novel F420-dependent Thioredoxin Reductase Gated by Low Potential FAD Susanti, Dwi; Loganathan, Usha; Mukhopadhyay, Biswarup Journal of Biological Chemistry, Vol. 291, Issue 44 https://doi.org/10.1074/jbc.M116.750208	journal	October 2016
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological Diversity Galagan, J. E. Genome Research, Vol. 12, Issue 4 https://doi.org/10.1101/gr.223902	journal	April 2002
Structure of the F ₄₂₀ H ₂ :quinone oxidoreductase of Archaeoglobus fulgidus: Identification and overproduction of the F ₄₂₀ H ₂ -oxidizing subunit Brüggemann, Holger; Falinski, Frank; Deppenmeier, Uwe European Journal of Biochemistry, Vol. 267, Issue 18 https://doi.org/10.1046/j.1432-1327.2000.01657.x	journal	September 2000
In vitro cell growth of marine archaeal-bacterial consortia during anaerobic oxidation of methane with sulfate Nauhaus, Katja; Albrecht, Melanie; Elvert, Marcus Environmental Microbiology, Vol. 9, Issue 1 https://doi.org/10.1111/j.1462-2920.2006.01127.x	journal	January 2007
The CryoEM Method MicroED as a Powerful Tool for Small Molecule Structure Determination Jones, Christopher G.; Martynowycz, Michael W.; Hattne, Johan ACS Central Science, Vol. 4, Issue 11 https://doi.org/10.1021/acscentsci.8b00760	journal	November 2018
Coenzyme F ₄₂₀ -Dependent Glucose-6-Phosphate Dehydrogenase-Coupled Polyglutamylation of Coenzyme F ₄₂₀ in Mycobacteria Purwantini, Endang; Loganathan, Usha; Mukhopadhyay, Biswarup Journal of Bacteriology, Vol. 200, Issue 23 https://doi.org/10.1128/JB.00375-18	journal	December 2018
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
Artificial electron acceptors decouple archaeal methane oxidation from sulfate reduction Scheller, S.; Yu, H.; Chadwick, G. L. Science, Vol. 351, Issue 6274 https://doi.org/10.1126/science.aad7154	journal	February 2016
Sipros/ProRata: a versatile informatics system for quantitative community proteomics Wang, Yingfeng; Ahn, Tae-Hyuk; Li, Zhou Bioinformatics, Vol. 29, Issue 16 https://doi.org/10.1093/bioinformatics/btt329	journal	June 2013
The oxidative and nitrosative stress defence network of Wolinella succinogenes: cytochrome c nitrite reductase mediates the stress response to nitrite, nitric oxide, hydroxylamine and hydrogen peroxide: Stress defence networks in W. succinogenes Kern, Melanie; Volz, Jennifer; Simon, Jörg Environmental Microbiology, Vol. 13, Issue 9 https://doi.org/10.1111/j.1462-2920.2011.02520.x	journal	June 2011
Spectroscopic investigation of the nickel-containing porphinoid cofactor F430. Comparison of the free cofactor in the +1, +2 and +3 oxidation states with the cofactor bound to methyl-coenzyme M reductase in the silent, red and ox forms Duin, Evert C.; Signor, Luca; Piskorski, Rafal JBIC Journal of Biological Inorganic Chemistry, Vol. 9, Issue 5 https://doi.org/10.1007/s00775-004-0549-9	journal	May 2004
The steady state kinetics of the NADH-dependent nitrite reductase from Escherichia coli K12. The reduction of single-electron acceptors Jackson, R. H.; Cole, J. A.; Cornish-Bowden, A. Biochemical Journal, Vol. 203, Issue 2 https://doi.org/10.1042/bj2030505	journal	May 1982
Nonlinear least-squares data fitting in Excel spreadsheets Kemmer, Gerdi; Keller, Sandro Nature Protocols, Vol. 5, Issue 2 https://doi.org/10.1038/nprot.2009.182	journal	January 2010
Purification and characterization of F420H2-dehydrogenase from Methanolobus tindarius Haase, Peter; Deppenmeier, Uwe; Blaut, Michael European Journal of Biochemistry, Vol. 203, Issue 3 https://doi.org/10.1111/j.1432-1033.1992.tb16579.x	journal	February 1992
Purification and properties of an F420H2 dehydrogenase from Methanosarcina mazei Gö1 Abken, H. FEMS Microbiology Letters, Vol. 154, Issue 2 https://doi.org/10.1016/S0378-1097(97)00330-3	journal	September 1997
Highly accurate protein structure prediction with AlphaFold Jumper, John; Evans, Richard; Pritzel, Alexander Nature https://doi.org/10.1038/s41586-021-03819-2	journal	July 2021
Mode of action uncovered for the specific reduction of methane emissions from ruminants by the small molecule 3-nitrooxypropanol Duin, Evert C.; Wagner, Tristan; Shima, Seigo Proceedings of the National Academy of Sciences, Vol. 113, Issue 22 https://doi.org/10.1073/pnas.1600298113	journal	May 2016
Reverse Methanogenesis and Respiration in Methanotrophic Archaea Timmers, Peer H. A.; Welte, Cornelia U.; Koehorst, Jasper J. Archaea, Vol. 2017 https://doi.org/10.1155/2017/1654237	journal	January 2017
l -Cysteine Desulfidase: An [4Fe-4S] Enzyme Isolated from Methanocaldococcus jannaschii That Catalyzes the Breakdown of l -Cysteine into Pyruvate, Ammonia, and Sulfide Tchong, Shih-I; Xu, Huimin; White, Robert H. Biochemistry, Vol. 44, Issue 5 https://doi.org/10.1021/bi0484769	journal	January 2005
High rates of denitrification and nitrate removal in cold seep sediments Bowles, Marshall; Joye, Samantha The ISME Journal, Vol. 5, Issue 3 https://doi.org/10.1038/ismej.2010.134	journal	October 2010
Evolution of Orbitrap Mass Spectrometry Instrumentation Eliuk, Shannon; Makarov, Alexander Annual Review of Analytical Chemistry, Vol. 8, Issue 1 https://doi.org/10.1146/annurev-anchem-071114-040325	journal	July 2015
Single cell activity reveals direct electron transfer in methanotrophic consortia McGlynn, Shawn E.; Chadwick, Grayson L.; Kempes, Christopher P. Nature, Vol. 526, Issue 7574 https://doi.org/10.1038/nature15512	journal	September 2015
A Metagenomics-Based Metabolic Model of Nitrate-Dependent Anaerobic Oxidation of Methane by Methanoperedens-Like Archaea Arshad, Arslan; Speth, Daan R.; de Graaf, Rob M. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01423	journal	December 2015
Glutamate synthase: a fascinating pathway from L-glutamine to L-glutamate van den Heuvel, R. H. H.; Curti, B.; Vanoni, M. A. Cellular and Molecular Life Sciences (CMLS), Vol. 61, Issue 6 https://doi.org/10.1007/s00018-003-3316-0	journal	March 2004
Comparative Genomics and Proteomic Analysis of Assimilatory Sulfate Reduction Pathways in Anaerobic Methanotrophic Archaea Yu, Hang; Susanti, Dwi; McGlynn, Shawn E. Frontiers in Microbiology, Vol. 9 https://doi.org/10.3389/fmicb.2018.02917	journal	December 2018
Immunological detection of enzymes for sulfate reduction in anaerobic methane-oxidizing consortia: Localization of enzymes for sulfate reduction Milucka, Jana; Widdel, Friedrich; Shima, Seigo Environmental Microbiology, Vol. 15, Issue 5 https://doi.org/10.1111/1462-2920.12003	journal	October 2012
A Genetic System for Methanocaldococcus jannaschii: An Evolutionary Deeply Rooted Hyperthermophilic Methanarchaeon Susanti, Dwi; Frazier, Mary C.; Mukhopadhyay, Biswarup Frontiers in Microbiology, Vol. 10 https://doi.org/10.3389/fmicb.2019.01256	journal	July 2019
In-gel digestion for mass spectrometric characterization of proteins and proteomes Shevchenko, Andrej; Tomas, Henrik; Havli, Jan Nature Protocols, Vol. 1, Issue 6 https://doi.org/10.1038/nprot.2006.468	journal	December 2006
Comparative genomics reveals electron transfer and syntrophic mechanisms differentiating methanotrophic and methanogenic archaea Chadwick, Grayson L.; Skennerton, Connor T.; Laso-Pérez, Rafael PLOS Biology, Vol. 20, Issue 1 https://doi.org/10.1371/journal.pbio.3001508	journal	January 2022
A genetic system for Archaea of the genus Methanosarcina: Liposome-mediated transformation and construction of shuttle vectors Metcalf, W. W.; Zhang, J. K.; Apolinario, E. Proceedings of the National Academy of Sciences, Vol. 94, Issue 6 https://doi.org/10.1073/pnas.94.6.2626	journal	March 1997
Anaerobic Oxidation of Methane: Progress with an Unknown Process Knittel, Katrin; Boetius, Antje Annual Review of Microbiology, Vol. 63, Issue 1 https://doi.org/10.1146/annurev.micro.61.080706.093130	journal	October 2009
A cryptic sulfur cycle driven by iron in the methane zone of marine sediment (Aarhus Bay, Denmark) Holmkvist, Lars; Ferdelman, Timothy G.; Jørgensen, Bo Barker Geochimica et Cosmochimica Acta, Vol. 75, Issue 12 https://doi.org/10.1016/j.gca.2011.03.033	journal	June 2011
Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, <i>Nitrososphaera viennensis</i> Kobayashi, Shun; Hira, Daisuke; Yoshida, Keitaro Microbes and Environments, Vol. 33, Issue 4 https://doi.org/10.1264/jsme2.ME18058	journal	January 2018
Oceanic Methane Biogeochemistry Reeburgh, William S. Chemical Reviews, Vol. 107, Issue 2 https://doi.org/10.1021/cr050362v	journal	February 2007
Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro. Metcalf, W. W.; Zhang, J. K.; Shi, X. Journal of Bacteriology, Vol. 178, Issue 19 https://doi.org/10.1128/jb.178.19.5797-5802.1996	journal	October 1996
Sulfate differentially stimulates but is not respired by diverse anaerobic methanotrophic archaea Yu, Hang; Skennerton, Connor T.; Chadwick, Grayson L. The ISME Journal, Vol. 16, Issue 1 https://doi.org/10.1038/s41396-021-01047-0	journal	July 2021
Spinach siroheme enzymes: isolation and characterization of ferredoxin-sulfite reductase and comparison of properties with ferredoxin-nitrite reductase Krueger, Rick J.; Siegel, Lewis M. Biochemistry, Vol. 21, Issue 12 https://doi.org/10.1021/bi00541a014	journal	June 1982
The F ₄₂₀ H ₂ Dehydrogenase from Methanosarcina mazei Is a Redox-driven Proton Pump Closely Related to NADH Dehydrogenases Bäumer, Sebastian; Ide, Tina; Jacobi, Carsten Journal of Biological Chemistry, Vol. 275, Issue 24 https://doi.org/10.1074/jbc.M000650200	journal	April 2000
Anaerobic oxidation of methane coupled to nitrate reduction in a novel archaeal lineage Haroon, Mohamed F.; Hu, Shihu; Shi, Ying Nature, Vol. 500, Issue 7464 https://doi.org/10.1038/nature12375	journal	July 2013
Sequence-structure analysis of FAD-containing proteins Dym, Orly; Eisenberg, David Protein Science, Vol. 10, Issue 9 https://doi.org/10.1110/ps.12801	journal	September 2001
Chromatographic determination of flavin derivatives in baker's yeast Gliszczyńska, Anna; Koziołowa, Anna Journal of Chromatography A, Vol. 822, Issue 1 https://doi.org/10.1016/S0021-9673(98)00557-3	journal	September 1998
Siroamide: A Prosthetic Group Isolated from Sulfite Reductases in the Genus Desulfovibrio Matthews, Jeffrey C.; Timkovich, Russell; Liu, Ming-Yih Biochemistry, Vol. 34, Issue 15 https://doi.org/10.1021/bi00015a039	journal	April 1995
Gene expression and ultrastructure of meso- and thermophilic methanotrophic consortia: Interspecies interaction within AOM consortia Krukenberg, Viola; Riedel, Dietmar; Gruber-Vodicka, Harald R. Environmental Microbiology, Vol. 20, Issue 5 https://doi.org/10.1111/1462-2920.14077	journal	April 2018
Reduced Nicotinamide Adenine Dinucleotide Phosphate-Sulfite Reductase of Enterobacteria Siegel, Lewis M.; Davis, Patricia S.; Kamin, Henry Journal of Biological Chemistry, Vol. 249, Issue 5 https://doi.org/10.1016/S0021-9258(19)42921-9	journal	March 1974
Target-Decoy Search Strategy for Mass Spectrometry-Based Proteomics Elias, Joshua E.; Gygi, Steven P. Methods in Molecular Biology https://doi.org/10.1007/978-1-60761-444-9_5	book	December 2009
Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group Meyerdierks, Anke; Kube, Michael; Kostadinov, Ivaylo Environmental Microbiology, Vol. 12, Issue 2 https://doi.org/10.1111/j.1462-2920.2009.02083.x	journal	February 2010
An Intertwined Evolutionary History of Methanogenic Archaea and Sulfate Reduction Susanti, Dwi; Mukhopadhyay, Biswarup PLoS ONE, Vol. 7, Issue 9 https://doi.org/10.1371/journal.pone.0045313	journal	September 2012
Thermophilic anaerobic oxidation of methane by marine microbial consortia Holler, Thomas; Widdel, Friedrich; Knittel, Katrin The ISME Journal, Vol. 5, Issue 12 https://doi.org/10.1038/ismej.2011.77	journal	June 2011
Activation of Methyl-SCoM Reductase to High Specific Activity after Treatment of Whole Cells with Sodium Sulfide ^† Becker, Donald F.; Ragsdale, Stephen W. Biochemistry, Vol. 37, Issue 8 https://doi.org/10.1021/bi972145x	journal	February 1998
Presence and diversity of anammox bacteria in cold hydrocarbon-rich seeps and hydrothermal vent sediments of the Guaymas Basin Russ, Lina; Kartal, Boran; op den Camp, Huub J. M. Frontiers in Microbiology, Vol. 4 https://doi.org/10.3389/fmicb.2013.00219	journal	January 2013
Aerobic purification of N ⁵ , N ¹⁰ -methylenetetrahydromethanopterin dehydrogenase, separated from N ⁵ , N ¹⁰ -methenyltetrahydromethanopterin cyclohydrolase, from Methanobacterium thermoautotrophicum strain Marburg Mukhopadhyay, Biswarup; Daniels, Lacy Canadian Journal of Microbiology, Vol. 35, Issue 4 https://doi.org/10.1139/m89-077	journal	April 1989
Effect of temperature on the spectral properties of coenzyme F420 and related compounds Purwantini, Endang; Mukhopadhyay, Biswarup; Spencer, Robin W. Analytical Biochemistry, Vol. 205, Issue 2 https://doi.org/10.1016/0003-2697(92)90446-e	journal	September 1992
Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade: SEEP-SRB1 associated with ANME-2 Schreiber, Lars; Holler, Thomas; Knittel, Katrin Environmental Microbiology https://doi.org/10.1111/j.1462-2920.2010.02275.x	journal	July 2010
Zero-valent sulphur is a key intermediate in marine methane oxidation Milucka, Jana; Ferdelman, Timothy G.; Polerecky, Lubos Nature, Vol. 491, Issue 7425 https://doi.org/10.1038/nature11656	journal	November 2012
The Crystal Structure of Desulfovibrio vulgaris Dissimilatory Sulfite Reductase Bound to DsrC Provides Novel Insights into the Mechanism of Sulfate Respiration Oliveira, Tânia F.; Vonrhein, Clemens; Matias, Pedro M. Journal of Biological Chemistry, Vol. 283, Issue 49 https://doi.org/10.1074/jbc.M805643200	journal	September 2008
Intercellular wiring enables electron transfer between methanotrophic archaea and bacteria Wegener, Gunter; Krukenberg, Viola; Riedel, Dietmar Nature, Vol. 526, Issue 7574 https://doi.org/10.1038/nature15733	journal	October 2015
Desulfoviridin, a multimeric-dissimilatory sulfite reductase from Desulfovibrio vulgaris (Hildenborough) Purification, characterization, kinetics and EPR studies Wolfe, Bonnie M.; Lui, Siu Man; Cowan, James A. European Journal of Biochemistry, Vol. 223, Issue 1 https://doi.org/10.1111/j.1432-1033.1994.tb18968.x	journal	July 1994
Energy Metabolism during Anaerobic Methane Oxidation in ANME Archaea McGlynn, Shawn E. Microbes and environments, Vol. 32, Issue 1 https://doi.org/10.1264/jsme2.ME16166	journal	January 2017
Reversing methanogenesis to capture methane for liquid biofuel precursors Soo, Valerie W. C.; McAnulty, Michael J.; Tripathi, Arti Microbial Cell Factories, Vol. 15, Issue 1 https://doi.org/10.1186/s12934-015-0397-z	journal	January 2016
A standardized archaeal taxonomy for the Genome Taxonomy Database Rinke, Christian; Chuvochina, Maria; Mussig, Aaron J. Nature Microbiology, Vol. 6, Issue 7 https://doi.org/10.1038/s41564-021-00918-8	journal	June 2021
Purification of a novel coenzyme F420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis Purwantini, E.; Daniels, L. Journal of Bacteriology, Vol. 178, Issue 10 https://doi.org/10.1128/jb.178.10.2861-2866.1996	journal	May 1996
MUSCLE: multiple sequence alignment with high accuracy and high throughput Edgar, R. C. Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797 https://doi.org/10.1093/nar/gkh340	journal	March 2004
Determination of iron with bathophenanthroline without deproteinisation Bouda, J. Clinica Chimica Acta, Vol. 21, Issue 1 https://doi.org/10.1016/0009-8981(68)90026-0	journal	July 1968
The nickel enzyme methyl-coenzyme M reductase from methanogenic archaea: In vitro induction of the nickel-based MCR-ox EPR signals from MCR-red2 Mahlert, Felix; Bauer, Carsten; Jaun, Bernhard JBIC Journal of Biological Inorganic Chemistry, Vol. 7, Issue 4-5 https://doi.org/10.1007/s00775-001-0325-z	journal	February 2002
A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding Bradford, M. Analytical Biochemistry, Vol. 72, Issue 1-2 https://doi.org/10.1006/abio.1976.9999	journal	May 1976
Protein Identification and Analysis Tools on the ExPASy Server Gasteiger, Elisabeth; Hoogland, Christine; Gattiker, Alexandre The Proteomics Protocols Handbook https://doi.org/10.1385/1-59259-890-0:571	book	January 2005
Reaction Cycle of the Dissimilatory Sulfite Reductase from Archaeoglobus fulgidus , Parey, Kristian; Warkentin, Eberhard; Kroneck, Peter M. H. Biochemistry, Vol. 49, Issue 41 https://doi.org/10.1021/bi100781f	journal	October 2010
Jalview Version 2--a multiple sequence alignment editor and analysis workbench Waterhouse, A. M.; Procter, J. B.; Martin, D. M. A. Bioinformatics, Vol. 25, Issue 9 https://doi.org/10.1093/bioinformatics/btp033	journal	January 2009
Regulation and Maturation of the Shewanella oneidensis Sulfite Reductase SirA Brockman, Kenneth L.; Shirodkar, Sheetal; Croft, Trevor J. Scientific Reports, Vol. 10, Issue 1 https://doi.org/10.1038/s41598-020-57587-6	journal	January 2020
De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy Mills, Deryck J.; Vitt, Stella; Strauss, Mike eLife, Vol. 2 https://doi.org/10.7554/eLife.00218	journal	March 2013
Methanotrophic archaea possessing diverging methane-oxidizing and electron-transporting pathways Wang, Feng-Ping; Zhang, Yu; Chen, Ying The ISME Journal, Vol. 8, Issue 5 https://doi.org/10.1038/ismej.2013.212	journal	December 2013
Iron oxides stimulate sulfate-driven anaerobic methane oxidation in seeps Sivan, Orit; Antler, Gilad; Turchyn, Alexandra V. Proceedings of the National Academy of Sciences, Vol. 111, Issue 40 https://doi.org/10.1073/pnas.1412269111	journal	September 2014
New methods for tightly regulated gene expression and highly efficient chromosomal integration of cloned genes for Methanosarcina species Guss, Adam M.; Rother, Michael; Zhang, Jun Kai Archaea, Vol. 2, Issue 3 https://doi.org/10.1155/2008/534081	journal	January 2008

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Title: A Reduced F 420 -Dependent Nitrite Reductase in an Anaerobic Methanotrophic Archaeon

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Title: A Reduced F ₄₂₀ -Dependent Nitrite Reductase in an Anaerobic Methanotrophic Archaeon