Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea

Shao, Nana; Fan, Yu; Chou, Chau-Wen; Yavari, Shadi; Williams, Robert V.; Amster, I. Jonathan; Brown, Stuart M.; Drake, Ian J.; Duin, Evert C.; Whitman, William B.; Liu, Yuchen

doi:10.1038/s42003-022-04057-6

Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea

Journal Article · Thu Oct 20 00:00:00 EDT 2022 · Communications Biology

DOI:https://doi.org/10.1038/s42003-022-04057-6· OSTI ID:1893952

; Fan, Yu; Chou, Chau-Wen; Yavari, Shadi; Williams, Robert V.; ; Brown, Stuart M.; Drake, Ian J.; Duin, Evert C.; ;

Abstract

Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme in methane metabolism, catalyzing the last step in methanogenesis and the first step in anaerobic methane oxidation. Divergent mcr and mcr -like genes have recently been identified in uncultured archaeal lineages. However, the assembly and biochemistry of MCRs from uncultured archaea remain largely unknown. Here we present an approach to study MCRs from uncultured archaea by heterologous expression in a methanogen, Methanococcus maripaludis . Promoter, operon structure, and temperature were important determinants for MCR production. Both recombinant methanococcal and ANME-2 MCR assembled with the host MCR forming hybrid complexes, whereas tested ANME-1 MCR and ethyl-coenzyme M reductase only formed homogenous complexes. Together with structural modeling, this suggests that ANME-2 and methanogen MCRs are structurally similar and their reaction directions are likely regulated by thermodynamics rather than intrinsic structural differences.

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Research Organization:: Univ. of Georgia, Athens, GA (United States). University of Georgia Research Foundation (UGARF)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Contributing Organization:: ExxonMobil Technology & Engineering Company

Grant/Contract Number:: SC0018028

OSTI ID:: 1893952

Alternate ID(s):: OSTI ID: 1888528

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 5; ISSN 2399-3642

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea

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