Fast MAS 1H–13C correlation NMR for structural investigations of plant cell walls

Phyo, Pyae; Hong, Mei

doi:10.1007/s10858-019-00277-x

Fast MAS ¹H–¹³C correlation NMR for structural investigations of plant cell walls

Journal Article · Wed Sep 18 00:00:00 EDT 2019 · Journal of Biomolecular NMR

DOI:https://doi.org/10.1007/s10858-019-00277-x· OSTI ID:1873745

Phyo, Pyae ^[1]; ^[2]

Massachusetts Inst. of Technology, Cambridge, MA (United States); Massachusetts Institute of Technology
Massachusetts Inst. of Technology, Cambridge, MA (United States)

Plant cell walls consist of a mixture of polysaccharides that render the cell wall a strong and dynamic material. Understanding the molecular structure and dynamics of wall polysaccharides is important for understanding and improving the properties of this energy-rich biomaterial. So far, solid-state NMR studies of cell wall structure and dynamics have solely relied on ¹³C chemical shifts measured from 2D and 3D correlation experiments. To increase the spectral resolution, sensitivity and upper limit of measurable distances, it is of interest to explore ¹H chemical shifts and ¹H-detected NMR experiments for analyzing cell walls. Here we demonstrate 2D and 3D ¹H–¹³C correlation experiments at both moderate and fast MAS frequencies of 10–50 kHz to resolve and assign ¹H chemical shifts of matrix polysaccharides in Arabidopsis primary cell walls. Here, both ¹³C-detected and ¹H-detected experiments are implemented and are shown to provide useful and complementary information. Using the assigned ¹H chemical shifts, we measured long-range correlations between matrix polysaccharides and cellulose using ¹H–¹H instead of ¹³C–¹³C spin diffusion, and the 2D experiments can be conducted with either ¹³C or ¹H detection.

View Accepted Manuscript (DOE)

Research Organization:: Pennsylvania State Univ., University Park, PA (United States); Massachusetts Inst. of Technology, Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1873745

Alternate ID(s):: OSTI ID: 1767548
OSTI ID: 22935916

Journal Information:: Journal of Biomolecular NMR, Journal Name: Journal of Biomolecular NMR Journal Issue: 12 Vol. 73; ISSN 0925-2738

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Fast MAS 1H–13C correlation NMR for structural investigations of plant cell walls

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