Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942

Carrieri, Damian; Jurista, Tracey; Yazvenko, Nina; Schafer Medina, Adan; Strickland, Devin; Roberts, James M.

doi:10.1016/j.algal.2021.102510

Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942

Journal Article · Mon Oct 25 00:00:00 EDT 2021 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2021.102510· OSTI ID:1869679

Carrieri, Damian ^[1]; Jurista, Tracey ^[2]; Yazvenko, Nina ^[2]; Schafer Medina, Adan ^[2]; Strickland, Devin ^[2]; Roberts, James M. ^[3]

Lumen Bioscience, Inc., Seattle, WA (United States); Matrix Genetics, LLC, Seattle, WA (United States); Lumen Bioscience, Seattle, WA, USA
Matrix Genetics, LLC, Seattle, WA (United States)
Lumen Bioscience, Inc., Seattle, WA (United States); Matrix Genetics, LLC, Seattle, WA (United States)

Many research groups have considered whether decreasing photosynthetic antenna content in cyanobacteria can improve photosynthetic oxygen evolution and biomass accumulation. In this work, we describe a genetic strategy for truncating light harvesting antennae based on engineered alterations in the expression of NblA, a native protein naturally used by cyanobacteria to disassemble phycobilisomes antennae in response to environmental changes. We show that if enforced overexpression is fine-tuned, it is possible to realize gains in photosynthesis in a model cyanobacterium Synechococcus elongatus PCC 7942. We also show that antennae truncation helps protect cells from photodamage and that a strain overexpressing NblA can outcompete wild type in a mixed population. We propose approaches for future applications of these findings.

View Accepted Manuscript (DOE)

Research Organization:: Lumen Bioscience, Inc., Seattle, WA (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Contributing Organization:: Lumen Bioscience, Inc., Seattle, WA (United States); Matrix Genetics, LLC, Seattle, WA (United States)

Grant/Contract Number:: EE0008120

OSTI ID:: 1869679

Alternate ID(s):: OSTI ID: 1829407

Journal Information:: Algal Research, Journal Name: Algal Research Vol. 60; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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