Self-assembly of {alpha}-helical polypeptides driven by complex coacervation.

Priftis, Dimitrios; Leon, Lorraine; Song, Ziyuan; Perry, Sarah L.; Margossian, Khatcher O.; Tropnikova, Anna; Cheng, Jianjun; Tirrell, Matthew

doi:10.1002/anie.201504861

Title: Self-assembly of {alpha}-helical polypeptides driven by complex coacervation.

Journal Article · Mon Sep 14 00:00:00 EDT 2015 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.201504861· OSTI ID:1868955

Priftis, Dimitrios; Leon, Lorraine; Song, Ziyuan; Perry, Sarah L.; Margossian, Khatcher O.; Tropnikova, Anna; Cheng, Jianjun; Tirrell, Matthew

Reported is the ability of alpha-helical polypeptides to self-assemble with oppositely-charged polypeptides to form liquid complexes while maintaining their alpha-helical secondary structure. Coupling the alpha-helical polypeptide to a neutral, hydrophilic polymer and subsequent complexation enables the formation of nanoscale coacervate-core micelles. While previous reports on polypeptide complexation demonstrated a critical dependence of the nature of the complex (liquid versus solid) on chirality, the alpha-helical structure of the positively charged polypeptide prevents the formation of beta-sheets, which would otherwise drive the assembly into a solid state, thereby, enabling coacervate formation between two chiral components. The higher charge density of the assembly, a result of the folding of the alpha-helical polypeptide, provides enhanced resistance to salts known to inhibit polypeptide complexation. The unique combination of properties of these materials can enhance the known potential of fluid polypeptide complexes for delivery of biologically relevant molecules.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science - Office of Basic Energy Sciences - Materials Sciences and Engineering Division

DOE Contract Number:: AC02-06CH11357

OSTI ID:: 1868955

Journal Information:: Angewandte Chemie (International Edition), Vol. 54, Issue 38

Country of Publication:: United States

Language:: English

References (50)

Thermodynamic Characterization of Polypeptide Complex Coacervation Priftis, Dimitrios; Laugel, Nicolas; Tirrell, Matthew Langmuir, Vol. 28, Issue 45 https://doi.org/10.1021/la302729r	journal	October 2012
Ideal Mixing in Polyelectrolyte Complexes and Multilayers: Entropy Driven Assembly Bucur, Claudiu B.; Sui, Zhijie; Schlenoff, Joseph B. Journal of the American Chemical Society, Vol. 128, Issue 42 https://doi.org/10.1021/ja064532c	journal	October 2006
Electrostatic Free Energy of Weakly Charged Macromolecules in Solution and Intermacromolecular Complexes Consisting of Oppositely Charged Polymers Biesheuvel, P. Maarten; Cohen Stuart, Martien A. Langmuir, Vol. 20, Issue 7 https://doi.org/10.1021/la036204l	journal	March 2004
Preparation of microcapsules by complex coacervation of gum Arabic and chitosan Butstraen, Chloé; Salaün, Fabien Carbohydrate Polymers, Vol. 99 https://doi.org/10.1016/j.carbpol.2013.09.006	journal	January 2014
Bio-inspired catechol chemistry: a new way to develop a re-moldable and injectable coacervate hydrogel Oh, Yeon Jeong; Cho, Il Hwan; Lee, Haeshin Chemical Communications, Vol. 48, Issue 97 https://doi.org/10.1039/c2cc36843a	journal	January 2012
Salt-Induced Disintegration of Lysozyme-Containing Polyelectrolyte Complex Micelles Lindhoud, Saskia; Voorhaar, Lenny; de Vries, Renko Langmuir, Vol. 25, Issue 19 https://doi.org/10.1021/la901591p	journal	August 2009
Complex Coacervation of Whey Proteins and Gum Arabic Weinbreck, F.; de Vries, R.; Schrooyen, P. Biomacromolecules, Vol. 4, Issue 2 https://doi.org/10.1021/bm025667n	journal	January 2003
Complex coacervation of poly(ethylene-imine)/polypeptide aqueous solutions: Thermodynamic and rheological characterization Priftis, Dimitrios; Megley, Katie; Laugel, Nicolas Journal of Colloid and Interface Science, Vol. 398 https://doi.org/10.1016/j.jcis.2013.01.055	journal	May 2013
Phase Behavior and Coacervation of Aqueous Poly(acrylic acid)−Poly(allylamine) Solutions Chollakup, Rungsima; Smitthipong, Wirasak; Eisenbach, Claus D. Macromolecules, Vol. 43, Issue 5 https://doi.org/10.1021/ma902144k	journal	March 2010
The Effect of Salt on the Complex Coacervation of Vinyl Polyelectrolytes Perry, Sarah; Li, Yue; Priftis, Dimitrios Polymers, Vol. 6, Issue 6 https://doi.org/10.3390/polym6061756	journal	June 2014
Phase behaviour and complex coacervation of aqueous polypeptide solutions Priftis, Dimitrios; Tirrell, Matthew Soft Matter, Vol. 8, Issue 36 https://doi.org/10.1039/C2SM25604E	journal	January 2012
Ionic polypeptides with unusual helical stability Lu, Hua; Wang, Jing; Bai, Yugang Nature Communications, Vol. 2, Issue 1 https://doi.org/10.1038/ncomms1209	journal	February 2011
Interfacial Energy of Polypeptide Complex Coacervates Measured via Capillary Adhesion Priftis, Dimitrios; Farina, Robert; Tirrell, Matthew Langmuir, Vol. 28, Issue 23 https://doi.org/10.1021/la300769d	journal	May 2012
Interfacial tension between a complex coacervate phase and its coexisting aqueous phase Spruijt, Evan; Sprakel, Joris; Cohen Stuart, Martien A. Soft Matter, Vol. 6, Issue 1 https://doi.org/10.1039/B911541B	journal	January 2010
Polyelectrolytes for cell encapsulation Bhatia, Surita R.; Khattak, Sarwat F.; Roberts, Susan C. Current Opinion in Colloid & Interface Science, Vol. 10, Issue 1-2 https://doi.org/10.1016/j.cocis.2005.05.004	journal	August 2005
Cement Proteins of the Tube-building Polychaete Phragmatopoma californica Zhao, Hua; Sun, Chengjun; Stewart, Russell J. Journal of Biological Chemistry, Vol. 280, Issue 52 https://doi.org/10.1074/jbc.M508457200	journal	October 2005
The tube cement of Phragmatopoma californica: a solid foam Stewart, Russell J.; Weaver, James C.; Morse, Daniel E. Journal of Experimental Biology, Vol. 207, Issue 26 https://doi.org/10.1242/jeb.01330	journal	December 2004
Complexation of Whey Proteins with Carrageenan Weinbreck, Fanny; Nieuwenhuijse, Hans; Robijn, Gerard W. Journal of Agricultural and Food Chemistry, Vol. 52, Issue 11 https://doi.org/10.1021/jf034969t	journal	May 2004
Tunable, High Modulus Hydrogels Driven by Ionic Coacervation Hunt, Jasmine N.; Feldman, Kathleen E.; Lynd, Nathaniel A. Advanced Materials, Vol. 23, Issue 20 https://doi.org/10.1002/adma.201004230	journal	April 2011
Effects of Polymer and Salt Concentration on the Structure and Properties of Triblock Copolymer Coacervate Hydrogels Krogstad, Daniel V.; Lynd, Nathaniel A.; Choi, Soo-Hyung Macromolecules, Vol. 46, Issue 4 https://doi.org/10.1021/ma302299r	journal	February 2013
Multiresponsive Reversible Gels Based on Charge-Driven Assembly Lemmers, Marc; Sprakel, Joris; Voets, Ilja K. Angewandte Chemie International Edition, Vol. 49, Issue 4 https://doi.org/10.1002/anie.200905515	journal	December 2009
Multiresponsive Reversible Gels Based on Charge-Driven Assembly Lemmers, Marc; Sprakel, Joris; Voets, Ilja K. Angewandte Chemie, Vol. 122, Issue 4 https://doi.org/10.1002/ange.200905515	journal	December 2009
Dual Stimuli-Responsive Poly( N -isopropylacrylamide)- b -poly( l -histidine) Chimeric Materials for the Controlled Delivery of Doxorubicin into Liver Carcinoma Johnson, Renjith P.; Jeong, Young−Il; John, Johnson V. Biomacromolecules, Vol. 14, Issue 5 https://doi.org/10.1021/bm400089m	journal	April 2013
Preparation and Characterization of Polyion Complex Micelles with a Novel Thermosensitive Poly(2-isopropyl-2-oxazoline) Shell via the Complexation of Oppositely Charged Block Ionomers ^† Park, Joon-Sik; Akiyama, Yoshitsugu; Yamasaki, Yuichi Langmuir, Vol. 23, Issue 1 https://doi.org/10.1021/la061431j	journal	January 2007
Block copolymer micelles for delivery of gene and related compounds Kakizawa, Yoshinori; Kataoka, Kazunori Advanced Drug Delivery Reviews, Vol. 54, Issue 2 https://doi.org/10.1016/S0169-409X(02)00017-0	journal	February 2002
Inhibition of atherosclerosis-promoting microRNAs via targeted polyelectrolyte complex micelles Kuo, Cheng-Hsiang; Leon, Lorraine; Chung, Eun Ji J. Mater. Chem. B, Vol. 2, Issue 46 https://doi.org/10.1039/C4TB00977K	journal	January 2014
Protein Folding: A Perspective from Theory and Experiment Dobson, Christopher M.; Šali, Andrej; Karplus, Martin Angewandte Chemie International Edition, Vol. 37, Issue 7 https://doi.org/10.1002/(SICI)1521-3773(19980420)37:7<868::AID-ANIE868>3.0.CO;2-H	journal	April 1998
Proteinfaltung aus theoretischer und experimenteller Sicht Dobson, Christopher M.; Šali, Andrej; Karplus, Martin Angewandte Chemie, Vol. 110, Issue 7 https://doi.org/10.1002/(SICI)1521-3757(19980403)110:7<908::AID-ANGE908>3.0.CO;2-0	journal	April 1998
Helix propensities of the amino acids measured in alanine-based peptides without helix-stabilizing side-chain interactions Chakrabartty, Avijit; Kortemme, Tanja; Baldwin, Robert L. Protein Science, Vol. 3, Issue 5 https://doi.org/10.1002/pro.5560030514	journal	May 1994
Chirality-selected phase behaviour in ionic polypeptide complexes Perry, Sarah L.; Leon, Lorraine; Hoffmann, Kyle Q. Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms7052	journal	January 2015
Light-Responsive Helical Polypeptides Capable of Reducing Toxicity and Unpacking DNA: Toward Nonviral Gene Delivery Yin, Lichen; Tang, Haoyu; Kim, Kyung Hoon Angewandte Chemie International Edition, Vol. 52, Issue 35 https://doi.org/10.1002/anie.201302820	journal	July 2013
Light‐Responsive Helical Polypeptides Capable of Reducing Toxicity and Unpacking DNA: Toward Nonviral Gene Delivery Yin, Lichen; Tang, Haoyu; Kim, Kyung Hoon Angewandte Chemie, Vol. 125, Issue 35 https://doi.org/10.1002/ange.201302820	journal	July 2013
Reactive and Bioactive Cationic α-Helical Polypeptide Template for Nonviral Gene Delivery Gabrielson, Nathan P.; Lu, Hua; Yin, Lichen Angewandte Chemie International Edition, Vol. 51, Issue 5 https://doi.org/10.1002/anie.201104262	journal	December 2011
Reactive and Bioactive Cationic α-Helical Polypeptide Template for Nonviral Gene Delivery Gabrielson, Nathan P.; Lu, Hua; Yin, Lichen Angewandte Chemie, Vol. 124, Issue 5 https://doi.org/10.1002/ange.201104262	journal	December 2011
The effect of side-chain functionality and hydrophobicity on the gene delivery capabilities of cationic helical polypeptides Zhang, Rujing; Zheng, Nan; Song, Ziyuan Biomaterials, Vol. 35, Issue 10 https://doi.org/10.1016/j.biomaterials.2013.12.097	journal	March 2014
Helical poly(arginine) mimics with superior cell-penetrating and molecular transporting properties Tang, Haoyu; Yin, Lichen; Kim, Kyung Hoon Chemical Science, Vol. 4, Issue 10 https://doi.org/10.1039/c3sc51328a	journal	January 2013
Rheological Properties of Mixtures of Oppositely Charged Polyelectrolytes. A Study of the Interactions between a Cationic Cellulose Ether and a Hydrophobically Modified Poly[sodium 2-(acrylamido)-2-methylpropanesulfonate] Liu, Roger C. W.; Morishima, Yotaro; Winnik, Françoise M. Polymer Journal, Vol. 34, Issue 5 https://doi.org/10.1295/polymj.34.340	journal	May 2002
Relaxation Dynamics at Different Time Scales in Electrostatic Complexes: Time-Salt Superposition Spruijt, Evan; Sprakel, Joris; Lemmers, Marc Physical Review Letters, Vol. 105, Issue 20 https://doi.org/10.1103/PhysRevLett.105.208301	journal	November 2010
Ternary, Tunable Polyelectrolyte Complex Fluids Driven by Complex Coacervation Priftis, Dimitrios; Xia, Xiaoxing; Margossian, Khatcher O. Macromolecules, Vol. 47, Issue 9 https://doi.org/10.1021/ma500245j	journal	April 2014
Phase separation in polyelectrolyte solutions. Theory of complex coacervation Overbeek, J. T. G.; Voorn, M. J. Journal of Cellular and Comparative Physiology, Vol. 49, Issue S1 https://doi.org/10.1002/jcp.1030490404	journal	May 1957
Formation of Polyion Complex Micelles in an Aqueous Milieu from a Pair of Oppositely-Charged Block Copolymers with Poly(ethylene glycol) Segments Harada, Atsushi; Kataoka, Kazunori Macromolecules, Vol. 28, Issue 15 https://doi.org/10.1021/ma00119a019	journal	July 1995
Soluble Stoichiometric Complexes from Poly( N -ethyl-4-vinylpyridinium) Cations and Poly(ethylene oxide)- block -polymethacrylate Anions Kabanov, Alexander V.; Bronich, Tatiana K.; Kabanov, Victor A. Macromolecules, Vol. 29, Issue 21 https://doi.org/10.1021/ma960120k	journal	January 1996
Water-Soluble Complexes Formed by Sodium Poly(4-styrenesulfonate) and a Poly(2-vinylpyridinium)- b lock -poly(ethyleneoxide) Copolymer Gohy, Jean-François; Varshney, Sunil K.; Antoun, Sayed Macromolecules, Vol. 33, Issue 25 https://doi.org/10.1021/ma001160y	journal	November 2000
Irena : tool suite for modeling and analysis of small-angle scattering Ilavsky, Jan; Jemian, Peter R. Journal of Applied Crystallography, Vol. 42, Issue 2 https://doi.org/10.1107/S0021889809002222	journal	February 2009
Effect of Charged Segment Length on Physicochemical Properties of Core−Shell Type Polyion Complex Micelles from Block Ionomers Harada, Atsushi; Kataoka, Kazunori Macromolecules, Vol. 36, Issue 13 https://doi.org/10.1021/ma025737i	journal	July 2003
Complex coacervate core micelles Voets, Ilja K.; de Keizer, Arie; Cohen Stuart, Martien A. Advances in Colloid and Interface Science, Vol. 147-148 https://doi.org/10.1016/j.cis.2008.09.012	journal	March 2009
Colloidal Stability of Aqueous Dispersions of Block Ionomer Complexes: Effects of Temperature and Salt Solomatin, Sergey V.; Bronich, Tatiana K.; Eisenberg, Adi Langmuir, Vol. 20, Issue 6 https://doi.org/10.1021/la034895f	journal	February 2004
Preparation and Characterization of Polyion Complex Micelles with Phosphobetaine Shells Nakai, Keita; Nishiuchi, Midori; Inoue, Masamichi Langmuir, Vol. 29, Issue 31 https://doi.org/10.1021/la401063b	journal	July 2013
Protein Encapsulation via Polypeptide Complex Coacervation Black, Katie A.; Priftis, Dimitrios; Perry, Sarah L. ACS Macro Letters, Vol. 3, Issue 10 https://doi.org/10.1021/mz500529v	journal	October 2014
Progress of drug-loaded polymeric micelles into clinical studies Cabral, Horacio; Kataoka, Kazunori Journal of Controlled Release, Vol. 190 https://doi.org/10.1016/j.jconrel.2014.06.042	journal	September 2014

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Title: Self-assembly of {alpha}-helical polypeptides driven by complex coacervation.

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