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Title: Single-domain antibodies neutralize ricin toxin intracellularly by blocking access to ribosomal P-st

Journal Article · · J. Biol. Chem.

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
DOE - Office Of Science; NIAID; National Institutes of Health (NIH)
OSTI ID:
1863990
Journal Information:
J. Biol. Chem., Vol. 298, Issue (4)
Country of Publication:
United States
Language:
ENGLISH

References (61)

The three-dimensional structure of ricin at 2.8 A. journal April 1987
Mechanism of action of the toxic lectins abrin and ricin journal June 1974
Intracellular Transport and Cytotoxicity of the Protein Toxin Ricin journal June 2019
Reductive activation of ricin and ricin A-chain immunotoxins by protein disulfide isomerase and thioredoxin reductase journal May 2004
Toxins Utilize the Endoplasmic Reticulum-Associated Protein Degradation Pathway in Their Intoxication Process journal March 2019
Protein disulphide-isomerase reduces ricin to its A and B chains in the endoplasmic reticulum journal October 2004
Dependence of Ricin Toxicity on Translocation of the Toxin A-chain from the Endoplasmic Reticulum to the Cytosol journal November 1999
The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins. journal April 1987
RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes. journal June 1987
Ribotoxic stress response: activation of the stress-activated protein kinase JNK1 by inhibitors of the peptidyl transferase reaction and by sequence-specific RNA damage to the alpha-sarcin/ricin loop in the 28S rRNA journal June 1997
Ribosomal stress-surveillance: three pathways is a magic number journal September 2020
Time-resolved cryo-EM visualizes ribosomal translocation with EF-G and GTP journal December 2021
The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. journal June 1988
Site-directed mutagenesis of ricin A-chain and implications for the mechanism of action journal March 1991
Interaction of elongation factors EF-G and EF-Tu with a conserved loop in 23S RNA journal July 1988
X-ray analysis of substrate analogs in the ricin A-chain active site journal October 1992
Structure of recombinant ricin A chain at 2.3 Å: Structure of rRTA journal March 1993
Structural analysis of ricin and implications for inhibitor design journal November 1996
How Ricin Damages the Ribosome journal April 2019
Targeting ricin to the ribosome journal July 2013
A Two-Step Binding Model Proposed for the Electrostatic Interactions of Ricin A Chain with Ribosomes journal May 2009
The P1/P2 proteins of the human ribosomal stalk are required for ribosome binding and depurination by ricin in human cells journal September 2012
A relatively low level of ribosome depurination by mutant forms of ricin toxin A chain can trigger protein synthesis inhibition, cell signaling and apoptosis in mammalian cells journal December 2012
Ricin uses arginine 235 as an anchor residue to bind to P-proteins of the ribosomal stalk journal February 2017
Structural insights into the interaction of the ribosomal P stalk protein P2 with a type II ribosome-inactivating protein ricin journal November 2016
Structures and Ribosomal Interaction of Ribosome-Inactivating Proteins journal November 2016
Peptide Mimics of the Ribosomal P Stalk Inhibit the Activity of Ricin A Chain by Preventing Ribosome Binding journal September 2018
Intracellular Neutralization of Ricin Toxin by Single-domain Antibodies Targeting the Active Site journal February 2020
A Supercluster of Neutralizing Epitopes at the Interface of Ricin’s Enzymatic (RTA) and Binding (RTB) Subunits journal November 2017
Structural Analysis of Toxin-Neutralizing, Single-Domain Antibodies that Bridge Ricin’s A-B Subunit Interface journal July 2021
Structural Analysis of Single Domain Antibodies Bound to a Second Neutralizing Hot Spot on Ricin Toxin's Enzymatic Subunit journal January 2017
Applications of Nanobodies journal February 2021
Crystal Structure of Ribosome-Inactivating Protein Ricin A Chain in Complex with the C-Terminal Peptide of the Ribosomal Stalk Protein P2 journal October 2016
Camelid single domain antibodies (VHHs) as neuronal cell intrabody binding agents and inhibitors of Clostridium botulinum neurotoxin (BoNT) proteases journal November 2010
Synthesis and Structural Characterization of Ricin Inhibitors Targeting Ribosome Binding Using Fragment-Based Methods and Structure-Based Design journal October 2021
Human ribosomal P1-P2 heterodimer represents an optimal docking site for ricin A chain with a prominent role for P1 C-terminus journal July 2017
Phosphorylation of the conserved C‐terminal domain of ribosomal P‐proteins impairs the mode of interaction with plant toxins journal August 2021
Leucine 232 and hydrophobic residues at the ribosomal P stalk binding site are critical for biological activity of ricin journal October 2019
Arginine Residues on the Opposite Side of the Active Site Stimulate the Catalysis of Ribosome Depurination by Ricin A Chain by Interacting with the P-protein Stalk journal September 2013
The ribosomal stalk is required for ribosome binding, depurination of the rRNA and cytotoxicity of ricin A chain in Saccharomyces cerevisiae journal December 2008
Ribosome-mediated Folding of Partially Unfolded Ricin A-chain journal March 2000
Cytosolic chaperones influence the fate of a toxin dislocated from the endoplasmic reticulum journal November 2008
Direct visualization of translational GTPase factor pool formed around the archaeal ribosomal P-stalk by high-speed AFM journal December 2020
Structural basis for the interaction of Shiga toxin 2a with a C-terminal peptide of ribosomal P stalk proteins journal November 2020
Engineering antibody fragments to fold in the absence of disulfide bonds journal February 2009
An intrinsically stable antibody scFv fragment can tolerate the loss of both disulfide bonds and fold correctly journal May 1998
Antibody scFv fragments without disulfide bonds, made by molecular evolution journal January 1998
Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies journal August 2021
Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2 journal November 2020
Dual Beneficial Effect of Interloop Disulfide Bond for Single Domain Antibody Fragments* journal January 2012
The structural basis of nanobody unfolding reversibility and thermoresistance journal May 2018
Slaying SARS-CoV-2 One (Single-domain) Antibody at a Time journal March 2021
Universal protection against influenza infection by a multidomain antibody to influenza hemagglutinin journal November 2018
A guide to: generation and design of nanobodies journal August 2020
mRNA mediates passive vaccination against infectious agents, toxins, and tumors journal August 2017
[20] Processing of X-ray diffraction data collected in oscillation mode book January 1997
Overview of the CCP 4 suite and current developments journal March 2011
Phaser crystallographic software journal July 2007
Features and development of Coot journal March 2010
PHENIX: a comprehensive Python-based system for macromolecular structure solution journal January 2010
Fine-Specificity Epitope Analysis Identifies Contact Points on Ricin Toxin Recognized by Protective Monoclonal Antibodies journal September 2018

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