Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories

Liu, Rongming; Liang, Liya; Stettner, Sean; Freed, Emily F.; Eckert, Carrie A.

doi:10.1016/B978-0-12-821477-0.00001-5

Chapter 7 - CRISPR-Based Tools for Microbial Cell Factories

Book · Sun Mar 07 23:00:00 EST 2021

DOI:https://doi.org/10.1016/B978-0-12-821477-0.00001-5· OSTI ID:1862943

Liu, Rongming; Liang, Liya; Stettner, Sean; Freed, Emily F.;

The development of microbial chassis for the production of a variety of biochemicals and biofuels is a growing area of research. How to efficiently manipulate genetic information to achieve optimal production of these compounds is a key area of focus in the field. In recent years, clustered regularly interspaced palindromic repeats (CRISPR) and its associated proteins (Cas) have become a popular strategy for gene editing and regulation in many organisms due to its versatility and efficacy. Here, we describe methods developed utilizing CRISPR-Cas systems for engineering microbial cell factories (e.g., bacteria and yeast) at the single gene to genome scale for mutagenesis and transcriptional regulation of target genes. Finally, we provide a perspective on the challenges and opportunities for the applications of advanced CRISPR-Cas-based tools for engineering microbial cell factories.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

DOE Contract Number:: AC36-08GO28308

OSTI ID:: 1862943

Report Number(s):: NREL/CH-2700-76795; MainId:9456; UUID:9276b7cc-e4f2-4954-bcb6-764820858de6; MainAdminID:19875

Country of Publication:: United States

Language:: English

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