Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein gene (HSPA2)
- Univ. of Washington, Seattle, WA (United States); and others
A genomic clone for the human heat shock protein (HSP) 70 gene located on chromosome 14 was isolated and sequenced. The gene, designated HSPA2, has a single open reading frame of 1917 bp that encodes a 639-amino acid protein with a predicted molecular weight of 70,030 Da. Analysis of the sequence indicates that HLPA2 is the human homologue of the murine Hsp 70-2 gene with 91.7% identity in the nucleotide coding sequence and 98.2% in the corresponding amino acid sequence. HSPA2 has less amino acid homology to other members of the human HSP70 gene family, 83.3% to the heat-inducible HSP70-1 gene and 86.1% with the human heat shock cognate gene HSC70. HSPA2 is constitutively expressed in most tissues, with very high levels in testis and skeletal muscle. Significant but lower levels are also expressed in ovary, small intestine, colon, brain, placenta, and kidney. A yeast artificial chromosome (YAC) clone containing HSPA2 (YAC741H4) that also contained the polymorphic marker D14S63 was identified. This 670-kb YAC was mapped to 14q24.1 by fluorescence in situ hybridization (FISH). Subsequent two-color FISH and genetic mapping placed HSPA2/D14S63 proximal to the markers D14S57 and D14S77. 50 refs., 3 figs., 1 tab.
- OSTI ID:
- 186057
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 23; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
AMINO ACID SEQUENCE
BIOLOGICAL MARKERS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ETIOLOGY
FLUORESCENCE
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOMES
MENTAL DISORDERS
MICE
NERVOUS SYSTEM DISEASES
NUCLEOTIDES
PATIENTS
PROTEINS
TISSUE DISTRIBUTION
YEASTS