A heat shock gene at 14q22: Expression, physical and linkage mapping
- Univ. of Toronto (Canada); and others
The heat shock proteins (HSP) are a group of homologous proteins encoded by a multigene family. Their function as chaperones in modulating the conformation of newly synthesized polypeptides appears to have been conserved throughout evolution. Their expression can be constitutive and/or increased in response to heat shock or metabolic stress of cells. However, some HSP do not follow this rule, as expression of mouse HSP70-2, is restricted to the male germ cell line and is not increased by heat shock. Several HSP have been described in the MHC (major histocompatibility complex) on chromosome 6. We have cloned and mapped, more precisely, a chromosome 14 HSP gene. We used a genomic probe (pH2.3) from one of the HSP genes present in the MHC that was shown previously by Southern hybridization to detect sequences on chromosome 14. Screening in a chromosome 14-specific cosmid library yielded one relevant Cos5-6 clone. This clone contains an HSP-like sequence, showing a high degree of homology with HSP genes from the MHC and HSP70-2 in the mouse. The localization of this new HSP sequence to 14q22 was established using a cell hybrid panel and FISH analysis. A pentanucleotide repeat motif, showing seven alleles, was also found present in the same clone. Testing of appropriate recombinant individuals from CEPH pedigrees indicates that most likely order of the markers is D14S75-D14S47-HSP-PYGL-D14S52-D14S66. We hypothesize that this Hsp-like sequence corresponds to the human counterpart of the mouse HSP70-2 gene that maps in an equivalent region on chromosome 12. Unlike the HSP70-2 expressed in testis, our gene was found to be expressed mainly in brain, muscle, heart and esophagus.
- OSTI ID:
- 133868
- Report Number(s):
- CONF-941009--
- Journal Information:
- American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
ANIMAL CELLS
BIOLOGICAL EVOLUTION
BIOLOGICAL MARKERS
BIOLOGICAL SHOCK
DNA HYBRIDIZATION
DNA-CLONING
FLUORESCENCE
GENE REGULATION
GENES
GENETIC MAPPING
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 14
HUMAN CHROMOSOME 6
NUCLEOTIDES
POLYPEPTIDES
PROBES
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION