Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants

Sun, Tai-ping

doi:10.1016/j.sbi.2020.12.013

Title: Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants

Journal Article · Mon Jan 18 00:00:00 EST 2021 · Current Opinion in Structural Biology

DOI:https://doi.org/10.1016/j.sbi.2020.12.013· OSTI ID:1853182

Sun, Tai-ping ^[1]

Duke Univ., Durham, NC (United States)

Here, in metazoans, protein O-fucosylation of Ser/Thr residues was only found in secreted or cell surface proteins, and this post-translational modification is catalyzed by ER-localized protein O-fucosyltransferases (POFUTs) in the GT65 family. Recently, a novel nucleocytoplasmic POFUT, SPINDLY (SPY), was identified in the reference plant Arabidopsis thaliana to modify nuclear transcription regulators DELLAs, revealing a new regulatory mechanism for gene expression. The paralog of AtSPY, SECRET AGENT (SEC), is an O-link-N-acetylglucosamine (GlcNAc) transferase (OGT), which O-GlcNAcylates Ser/Thr residues of target proteins. Both AtSPY and AtSEC are tetratricopeptide repeat-domain-containing glycosyltransferases in the GT41 family. The discovery that AtSPY is a POFUT clarified decades of miss-classification of AtSPY as an OGT. SPY and SEC play pleiotropic roles in plant development, and the interactions between SPY and SEC are complex. SPY-like genes are conserved in diverse organisms, except in fungi and metazoans, suggesting that O-fucosylation is a common mechanism in modulating intracellular protein functions.

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Research Organization:: Duke Univ., Durham, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF); USDA

Grant/Contract Number:: SC0019393; 2R01 GM100051-05A1; MCB-1818161; 2018-67013-27395

OSTI ID:: 1853182

Alternate ID(s):: OSTI ID: 1778488

Journal Information:: Current Opinion in Structural Biology, Vol. 68, Issue C; ISSN 0959-440X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
protein O-fucosylation
protein O-GlcNAcylation
nucleocytoplasmic protein O-fucosylation
SPINDLY
POFUT

Title: Novel nucleocytoplasmic protein O-fucosylation by SPINDLY regulates diverse developmental processes in plants

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