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Title: An important role of L–fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity

Abstract

Plants mount coordinated immune responses to defend against pathogens. However, the cellular components required for plant immunity are not fully understood. The jasmonatemimicking coronatine (COR) toxin produced by Pseudomonas syringae pv. tomato (Pst) DC3000 functions to overcome plant immunity. We previously isolated eight Arabidopsis (scord) mutants that exhibit increased susceptibility to a COR-deficient mutant of Pst DC3000. Among them, the scord6 mutant exhibits defects both in stomatal closure response and in restricting bacterial multiplication inside the apoplast. However, the identity of SCORD6 remained elusive. In this study, we aim to identify the SCORD6 gene. Here, we identified SCORD6 via next-generation sequencing and found it to be MURUS1 (MUR1), which is involved in the biosynthesis of GDP-L Discovery of SCORD6 as MUR1 led to a series of experiments that revealed a multifaceted role of -fucose. L-fucose biosynthesis in stomatal and apoplastic defenses as well as in pattern-triggered immunity and effector-triggered immunity, including glycosylation of pattern-recognition receptors. Furthermore, compromised stomatal and/or apoplastic defenses were observed in mutants of several fucosyltransferases with specific substrates (e.g., O-glycan, N-glycan or the DELLA transcriptional repressors). Collectively, these results uncover a novel and broad role of L-fucose and protein fucosylation in plant immuinity.

Authors:
ORCiD logo [1]; ORCiD logo [1];  [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Department of Energy Plant Research Laboratory, East Lansing, MI (United States); Michigan State University East Lansing, MI (United States)
  2. Michigan State University East Lansing, MI (United States)
Publication Date:
Research Org.:
Michigan State University, East Lansing, MI (United States). MSU DOE Plant Research Lab
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); USDA
OSTI Identifier:
1671329
Alternate Identifier(s):
OSTI ID: 1502266
Grant/Contract Number:  
FG02-91ER20021; IOS‐1557437; 2015‐67017‐23360; 2017‐67017‐26180
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Volume: 222; Journal Issue: 2; Journal ID: ISSN 0028-646X
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
Coronatine; fucosylation; jasmonate; L‐fucose; plant immunity; stomata

Citation Formats

Zhang, Li, Paasch, Bradley C., Chen, Jin, Day, Brad, and He, Sheng Yang. An important role of L–fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. United States: N. p., 2018. Web. doi:10.1111/nph.15639.
Zhang, Li, Paasch, Bradley C., Chen, Jin, Day, Brad, & He, Sheng Yang. An important role of L–fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity. United States. https://doi.org/10.1111/nph.15639
Zhang, Li, Paasch, Bradley C., Chen, Jin, Day, Brad, and He, Sheng Yang. Sat . "An important role of L–fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity". United States. https://doi.org/10.1111/nph.15639. https://www.osti.gov/servlets/purl/1671329.
@article{osti_1671329,
title = {An important role of L–fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity},
author = {Zhang, Li and Paasch, Bradley C. and Chen, Jin and Day, Brad and He, Sheng Yang},
abstractNote = {Plants mount coordinated immune responses to defend against pathogens. However, the cellular components required for plant immunity are not fully understood. The jasmonatemimicking coronatine (COR) toxin produced by Pseudomonas syringae pv. tomato (Pst) DC3000 functions to overcome plant immunity. We previously isolated eight Arabidopsis (scord) mutants that exhibit increased susceptibility to a COR-deficient mutant of Pst DC3000. Among them, the scord6 mutant exhibits defects both in stomatal closure response and in restricting bacterial multiplication inside the apoplast. However, the identity of SCORD6 remained elusive. In this study, we aim to identify the SCORD6 gene. Here, we identified SCORD6 via next-generation sequencing and found it to be MURUS1 (MUR1), which is involved in the biosynthesis of GDP-L Discovery of SCORD6 as MUR1 led to a series of experiments that revealed a multifaceted role of -fucose. L-fucose biosynthesis in stomatal and apoplastic defenses as well as in pattern-triggered immunity and effector-triggered immunity, including glycosylation of pattern-recognition receptors. Furthermore, compromised stomatal and/or apoplastic defenses were observed in mutants of several fucosyltransferases with specific substrates (e.g., O-glycan, N-glycan or the DELLA transcriptional repressors). Collectively, these results uncover a novel and broad role of L-fucose and protein fucosylation in plant immuinity.},
doi = {10.1111/nph.15639},
url = {https://www.osti.gov/biblio/1671329}, journal = {New Phytologist},
issn = {0028-646X},
number = 2,
volume = 222,
place = {United States},
year = {2018},
month = {12}
}

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    Works referencing / citing this record:

    MUR1‐mediated cell‐wall fucosylation is required for freezing tolerance in Arabidopsis thaliana
    journal, September 2019