Arabidopsis bZIP11 Is a Susceptibility Factor During Pseudomonas syringae Infection
[1];
[3];
[7]
- Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92507, U.S.A.; Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, U.S.A.; Department of Biology, Stanford University, Stanford, CA 94305, U.S.A.
- Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, U.S.A.; Molecular Plant Physiology, Department of Biology, Utrecht University, Utrecht, The Netherlands
- Department of Biology, Stanford University, Stanford, CA 94305, U.S.A.
- Molecular Plant Physiology, Department of Biology, Utrecht University, Utrecht, The Netherlands
- Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, U.S.A.; Department of Molecular Biology, Princeton University, 119 Lewis Thomas Laboratory, Washington Road, Princeton, NJ, U.S.A.
- Molecular Plant Physiology, Department of Biology, Utrecht University, Utrecht, The Netherlands; Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, Umeå, Sweden
- Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, U.S.A.; Department of Biology, Stanford University, Stanford, CA 94305, U.S.A.; Molecular Physiology, Heinrich Heine Universität, 40225 Düsseldorf, Germany
The induction of plant nutrient secretion systems is critical for successful pathogen infection. Some bacterial pathogens (e.g., Xanthomonas spp.) use transcription activator-like (TAL) effectors to induce transcription of SWEET sucrose efflux transporters. Pseudomonas syringae pv. tomato strain DC3000 lacks TAL effectors yet is able to induce multiple SWEETs in Arabidopsis thaliana by unknown mechanisms. Because bacteria require other nutrients in addition to sugars for efficient reproduction, we hypothesized that Pseudomonas spp. may depend on host transcription factors involved in secretory programs to increase access to essential nutrients. Bioinformatic analyses identified the Arabidopsis basic-leucine zipper transcription factor bZIP11 as a potential regulator of nutrient transporters, including SWEETs and UmamiT amino acid transporters. Inducible downregulation of bZIP11 expression in Arabidopsis resulted in reduced growth of P. syringae pv. tomato strain DC3000, whereas inducible overexpression of bZIP11 resulted in increased bacterial growth, supporting the hypothesis that bZIP11-regulated transcription programs are essential for maximal pathogen titer in leaves. Our data are consistent with a model in which a pathogen alters host transcription factor expression upstream of secretory transcription networks to promote nutrient efflux from host cells. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
- Research Organization:
- Carnegie Inst. of Washington, Washington, DC (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC)
- DOE Contract Number:
- FG02-04ER15542
- OSTI ID:
- 1849501
- Journal Information:
- Molecular Plant-Microbe Interactions, Vol. 34, Issue 4; ISSN 0894-0282
- Publisher:
- APS Press - International Society for Molecular Plant-Microbe Interactions
- Country of Publication:
- United States
- Language:
- English
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