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Title: Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

Abstract

Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.

Authors:
 [1];  [2];  [2];  [1]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Howard Hughes Medical Inst.
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Publication Date:
Research Org.:
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1607990
Grant/Contract Number:  
FG02-91ER20021
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Methods in Molecular Biology (Online)
Additional Journal Information:
Journal Volume: 1531; Related Information: Chapter 12; Journal ID: ISSN 1940-6029
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Plant pathogen; Bacterial pathogenesis; Type III secretion; Plant immunity; Tobacco; Arabidopsis thaliana; Agrobacterium; Confocal microscopy

Citation Formats

Aung, Kyaw, Xin, Xiufang, Mecey, Christy, and He, Sheng Yang. Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants. United States: N. p., 2016. Web. doi:10.1007/978-1-4939-6649-3_12.
Aung, Kyaw, Xin, Xiufang, Mecey, Christy, & He, Sheng Yang. Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants. United States. doi:10.1007/978-1-4939-6649-3_12.
Aung, Kyaw, Xin, Xiufang, Mecey, Christy, and He, Sheng Yang. Sat . "Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants". United States. doi:10.1007/978-1-4939-6649-3_12. https://www.osti.gov/servlets/purl/1607990.
@article{osti_1607990,
title = {Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants},
author = {Aung, Kyaw and Xin, Xiufang and Mecey, Christy and He, Sheng Yang},
abstractNote = {Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.},
doi = {10.1007/978-1-4939-6649-3_12},
journal = {Methods in Molecular Biology (Online)},
issn = {1940-6029},
number = ,
volume = 1531,
place = {United States},
year = {2016},
month = {11}
}

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Works referenced in this record:

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