Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

Aung, Kyaw; Xin, Xiufang; Mecey, Christy; He, Sheng Yang

doi:10.1007/978-1-4939-6649-3_12

Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

Journal Article · Sat Nov 12 00:00:00 EST 2016 · Methods in Molecular Biology (Online)

DOI:https://doi.org/10.1007/978-1-4939-6649-3_12· OSTI ID:1607990

Aung, Kyaw ^[1]; Xin, Xiufang ^[2]; Mecey, Christy ^[2]; He, Sheng Yang ^[3]

Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Howard Hughes Medical Inst.; MSU DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Howard Hughes Medical Inst.

Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1607990

Journal Information:: Methods in Molecular Biology (Online), Journal Name: Methods in Molecular Biology (Online) Vol. 1531; ISSN 1940-6029

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Agrobacterium
Arabidopsis thaliana
Bacterial pathogenesis
Confocal microscopy
Plant immunity
Plant pathogen
Tobacco
Type III secretion

Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants

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