U.S. Department of EnergyOffice of Scientific and Technical Information
Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants
Abstract
Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.
- Authors:
-
- Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory and Howard Hughes Medical Inst.
- Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1607990
- Grant/Contract Number:
- FG02-91ER20021
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Methods in Molecular Biology (Online)
- Additional Journal Information:
- Journal Volume: 1531; Related Information: Chapter 12; Journal ID: ISSN 1940-6029
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant pathogen; Bacterial pathogenesis; Type III secretion; Plant immunity; Tobacco; Arabidopsis thaliana; Agrobacterium; Confocal microscopy
Citation Formats
Aung, Kyaw, Xin, Xiufang, Mecey, Christy, and He, Sheng Yang. Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants. United States: N. p., 2016.
Web. doi:10.1007/978-1-4939-6649-3_12.
Aung, Kyaw, Xin, Xiufang, Mecey, Christy, & He, Sheng Yang. Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants. United States. https://doi.org/10.1007/978-1-4939-6649-3_12
Aung, Kyaw, Xin, Xiufang, Mecey, Christy, and He, Sheng Yang. 2016.
"Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants". United States. https://doi.org/10.1007/978-1-4939-6649-3_12. https://www.osti.gov/servlets/purl/1607990.
@article{osti_1607990,
title = {Subcellular Localization of Pseudomonas syringae pv. tomato Effector Proteins in Plants},
author = {Aung, Kyaw and Xin, Xiufang and Mecey, Christy and He, Sheng Yang},
abstractNote = {Animal and plant pathogenic bacteria use type III secretion systems to translocate proteinaceous effectors to subvert innate immunity of their host organisms. Type III secretion/effector system is a crucial pathogenicity factor in many bacterial pathogens of plants and animals. Pseudomonas syringae pv. tomato (Pst) DC3000 injects a total of 36 protein effectors, which target a variety of host proteins. Studies of a subset of Pst DC3000 effectors demonstrated that bacterial effectors, once inside the host cell, are localized to different subcellular compartments, including plasma membrane, cytoplasm, mitochondria, chloroplast, and Trans-Golgi network, to carry out their virulence functions. Identifying the subcellular localization of bacterial effector proteins in host cells could provide substantial clues to understanding the molecular and cellular bases of the virulence activities of effector proteins. In this chapter, we present methods for transient or stable expression of bacterial effector proteins in tobacco and/or Arabidopsis thaliana for live cell imaging as well as confirming the subcellular localization in plants using fluorescent organelle markers or chemical treatment.},
doi = {10.1007/978-1-4939-6649-3_12},
url = {https://www.osti.gov/biblio/1607990},
journal = {Methods in Molecular Biology (Online)},
issn = {1940-6029},
number = ,
volume = 1531,
place = {United States},
year = {Sat Nov 12 00:00:00 EST 2016},
month = {Sat Nov 12 00:00:00 EST 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 11 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean.
journal, January 1991
- Whalen, M. C.; Innes, R. W.; Bent, A. F.
- The Plant Cell, Vol. 3, Issue 1
Rapid, combinatorial analysis of membrane compartments in intact plants with a multicolor marker set
journal, July 2009
- Geldner, Niko; Dénervaud-Tendon, Valérie; Hyman, Derek L.
- The Plant Journal, Vol. 59, Issue 1
Effector-triggered immunity blocks pathogen degradation of an immunity-associated vesicle traffic regulator in Arabidopsis
journal, June 2011
- Nomura, K.; Mecey, C.; Lee, Y. -N.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 26
T-DNA Binary Vectors and Systems
journal, February 2008
- Lee, Lan-Ying; Gelvin, Stanton B.
- Plant Physiology, Vol. 146, Issue 2
A guide to choosing fluorescent proteins
journal, November 2005
- Shaner, Nathan C.; Steinbach, Paul A.; Tsien, Roger Y.
- Nature Methods, Vol. 2, Issue 12
In-Depth Proteome Analysis of Arabidopsis Leaf Peroxisomes Combined with in Vivo Subcellular Targeting Verification Indicates Novel Metabolic and Regulatory Functions of Peroxisomes
journal, March 2009
- Reumann, Sigrun; Quan, Sheng; Aung, Kyaw
- Plant Physiology, Vol. 150, Issue 1
Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation
journal, July 2007
- Nakagawa, Tsuyoshi; Kurose, Takayuki; Hino, Takeshi
- Journal of Bioscience and Bioengineering, Vol. 104, Issue 1
A type III effector ADP-ribosylates RNA-binding proteins and quells plant immunity
journal, April 2007
- Fu, Zheng Qing; Guo, Ming; Jeong, Byeong-ryool
- Nature, Vol. 447, Issue 7142
Gateway-compatible vectors for plant functional genomics and proteomics
journal, February 2006
- Earley, Keith W.; Haag, Jeremy R.; Pontes, Olga
- The Plant Journal, Vol. 45, Issue 4
The Pseudomonas syringae type III effector HopD1 suppresses effector-triggered immunity, localizes to the endoplasmic reticulum, and targets the Arabidopsis transcription factor NTL9
journal, December 2013
- Block, Anna; Toruño, Tania Y.; Elowsky, Christian G.
- New Phytologist, Vol. 201, Issue 4
A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses: P. syringae HopN1 targets photosynthesis
journal, February 2012
- Rodríguez-Herva, José J.; González-Melendi, Pablo; Cuartas-Lanza, Raquel
- Cellular Microbiology, Vol. 14, Issue 5
A J Domain Virulence Effector of Pseudomonas syringae Remodels Host Chloroplasts and Suppresses Defenses
journal, March 2007
- Jelenska, Joanna; Yao, Nan; Vinatzer, Boris A.
- Current Biology, Vol. 17, Issue 6
A multicolored set of in vivo organelle markers for co-localization studies in Arabidopsis and other plants: Fluorescent organelle markers
journal, August 2007
- Nelson, Brook K.; Cai, Xue; Nebenführ, Andreas
- The Plant Journal, Vol. 51, Issue 6
Phosphorylation of HopQ1, a Type III Effector from Pseudomonas syringae , Creates a Binding Site for Host 14-3-3 Proteins
journal, February 2013
- Giska, Fabian; Lichocka, Małgorzata; Piechocki, Marcin
- Plant Physiology, Vol. 161, Issue 4
Distinct Pseudomonas type-III effectors use a cleavable transit peptide to target chloroplasts
journal, December 2013
- Li, Guangyong; Froehlich, John E.; Elowsky, Christian
- The Plant Journal, Vol. 77, Issue 2
The Pseudomonas syringae type III effector HopG1 targets mitochondria, alters plant development and suppresses plant innate immunity
journal, March 2010
- Block, Anna; Guo, Ming; Li, Guangyong
- Cellular Microbiology, Vol. 12, Issue 3
Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Polymutants Reveal an Interplay between HopAD1 and AvrPtoB
journal, June 2015
- Wei, Hai-Lei; Chakravarthy, Suma; Mathieu, Johannes
- Cell Host & Microbe, Vol. 17, Issue 6
Avoiding the Ends: Internal Epitope Tagging of Proteins Using Transposon Tn7
journal, March 2015
- Zordan, R. E.; Beliveau, B. J.; Trow, J. A.
- Genetics, Vol. 200, Issue 1
Pseudomonas syringae Effector Avirulence Protein E Localizes to the Host Plasma Membrane and Down-Regulates the Expression of the NONRACE-SPECIFIC DISEASE RESISTANCE1/HARPIN-INDUCED1-LIKE13 Gene Required for Antibacterial Immunity in Arabidopsis
journal, July 2015
- Xin, Xiu-Fang; Nomura, Kinya; Ding, Xinhua
- Plant Physiology, Vol. 169, Issue 1