KIN3 impacts arbuscular mycorrhizal symbiosis and promotes fungal colonisation in Medicago truncatula

Irving, Thomas B.; Chakraborty, Sanhita; Ivanov, Sergey; Schultze, Michael; Mysore, Kirankumar S.; Harrison, Maria J.; Ané, Jean‐Michel

doi:10.1111/tpj.15685

Title: KIN3 impacts arbuscular mycorrhizal symbiosis and promotes fungal colonisation in Medicago truncatula

Journal Article · Sat Mar 05 00:00:00 EST 2022 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.15685· OSTI ID:1848028

^[1]; Chakraborty, Sanhita ^[2]; Ivanov, Sergey ^[3]; Schultze, Michael ^[4];

^[5]; Harrison, Maria J. ^[3];

^[6]

Crop Science Centre University of Cambridge Cambridge CB3 0LE UK
Department of Bacteriology University of Wisconsin‐Madison Madison WI 53706 USA
Boyce Thompson Institute for Plant Research Cornell University Ithaca NY 14850 USA
Department of Biology (Ret.) University of York York YO10 5DD UK
Oklahoma State University Ardmore OK 74078 USA
Department of Bacteriology University of Wisconsin‐Madison Madison WI 53706 USA, Department of Agronomy University of Wisconsin‐Madison Madison WI 53706 USA

Summary Arbuscular mycorrhizal fungi help their host plant in the acquisition of nutrients, and this association is itself impacted by soil nutrient levels. High phosphorus levels inhibit the symbiosis, whereas high nitrogen levels enhance it. The genetic mechanisms regulating the symbiosis in response to soil nutrients are poorly understood. Here, we characterised the symbiotic phenotypes in four Medicago truncatula Tnt1 ‐insertion mutants affected in arbuscular mycorrhizal colonisation. We located their Tnt1 insertions and identified alleles for two genes known to be involved in mycorrhization, RAM1 and KIN3 . We compared the effects of the kin3‐2 and ram1‐4 mutations on gene expression, revealing that the two genes alter the expression of overlapping but not identical gene sets, suggesting that RAM1 acts upstream of KIN3. Additionally, KIN3 appears to be involved in the suppression of plant defences in response to the fungal symbiont. KIN3 is located on the endoplasmic reticulum of arbuscule‐containing cortical cells, and kin3‐2 mutants plants hosted significantly fewer arbuscules than the wild type. KIN3 plays an essential role in the symbiotic response to soil nitrogen levels, as, contrary to wild‐type plants, the kin3‐2 mutant did not exhibit increased root colonisation under high nitrogen.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: DE‐SC0018247

OSTI ID:: 1848028

Journal Information:: The Plant Journal, Journal Name: The Plant Journal Vol. 110 Journal Issue: 2; ISSN 0960-7412

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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