Reprogramming sphingolipid glycosylation is required for endosymbiont persistence in Medicago truncatula

Moore, William M.; Chan, Candace; Ishikawa, Toshiki; Rennie, Emilie A.; Wipf, Heidi M. -L.; Benites, Veronica; Kawai-Yamada, Maki; Mortimer, Jenny C.; Scheller, Henrik V.

doi:10.1016/j.cub.2021.03.067

Title: Reprogramming sphingolipid glycosylation is required for endosymbiont persistence in Medicago truncatula

Journal Article · Tue Jun 01 00:00:00 EDT 2021 · Current Biology

DOI:https://doi.org/10.1016/j.cub.2021.03.067· OSTI ID:1777615

Moore, William M.; Chan, Candace; Ishikawa, Toshiki; Rennie, Emilie A.; Wipf, Heidi M. -L.; Benites, Veronica; Kawai-Yamada, Maki; Mortimer, Jenny C.; Scheller, Henrik V.

Plant endosymbiosis relies on the development of specialized membranes that encapsulate the endosymbiont and facilitate nutrient exchange. However, the identity and function of lipids within these membrane interfaces is largely unknown. Here, we identify GLUCOSAMINE INOSITOL PHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) as a sphingolipid glycosyltransferase highly expressed in Medicago truncatula root nodules and roots colonized by arbuscular mycorrhizal (AM) fungi and further demonstrate that this enzyme functions in the synthesis of N-acetyl-glucosamine-decorated glycosyl inositol phosphoryl ceramides (GIPCs) in planta. MtGINT1 expression was developmentally regulated in symbiotic tissues associated with the development of symbiosome and periarbuscular membranes. RNAi silencing of MtGINT1 did not affect overall root growth but strongly impaired nodulation and AM symbiosis, resulting in the senescence of symbiosomes and arbuscules. Our results indicate that, although M. truncatula root sphingolipidome predominantly consists of hexose-decorated GIPCs, local reprogramming of GIPC glycosylation by MtGINT1 is required for the persistence of endosymbionts within the plant cell.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); JSPS KAKENHI

Grant/Contract Number:: AC02-05CH11231; DGE 1106400; 17K15411; 26292190

OSTI ID:: 1777615

Alternate ID(s):: OSTI ID: 1783142

Journal Information:: Current Biology, Journal Name: Current Biology Vol. 31 Journal Issue: 11; ISSN 0960-9822

Publisher:: ElsevierCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
GIPC
glycosyltransferase
Medicago truncatula
Sinorhizobium meliloti
Rhizophagus irregularis
sphingolipid
symbiosis

Title: Reprogramming sphingolipid glycosylation is required for endosymbiont persistence in Medicago truncatula

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