Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation

Patent · Tue Sep 28 04:00:00 EDT 2021

OSTI ID:1840498

Shelley, Jacob T.; Williams, Kelsey L.; Hieftje, Gary M.; Schwartz, Andrew J.

A solution-cathode glow discharge mass spectrometry (SCGD-MS) apparatus comprises a SCGD source and a mass spectrometer. The SCGD source may comprise conductive rods, a power source, and a capillary. A method for ionizing an analyte comprises flowing an electrically conductive liquid onto a conductive rod, applying an electric potential to a second conductive rod such that a plasma discharge forms between the first conductive rod and the electrically conductive liquid to produce ions, and separating the ions in a mass spectrometer. The analyte may be a polypeptide that may be contacted with trypsin. The analyte may be a solid, liquid, gas, chemical complex, or ion in solution. The method may comprise sequencing the polypeptide.

• Research Organization: Indiana Univ., Indianapolis, IN (United States); Kent State Univ., OH (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: FG02-98ER14890
• Assignee: Indiana University Research and Technology Corporation (Indianapolis, IN); Kent State University (Kent, OH)
• Patent Number(s): 11,133,170
• Application Number: 16/997,061
• OSTI ID: 1840498
• Country of Publication: United States
• Language: English