Atmospheric-pressure ionization and fragmentation of molecules for structural elucidation
A solution-cathode glow discharge mass spectrometry (SCGD-MS) apparatus comprises a SCGD source and a mass spectrometer. The SCGD source may comprise conductive rods, a power source, and a capillary. A method for ionizing an analyte comprises flowing an electrically conductive liquid onto a conductive rod, applying an electric potential to a second conductive rod such that a plasma discharge forms between the first conductive rod and the electrically conductive liquid to produce ions, and separating the ions in a mass spectrometer. The analyte may be a polypeptide that may be contacted with trypsin. The analyte may be a solid, liquid, gas, chemical complex, or ion in solution. The method may comprise sequencing the polypeptide.
- Research Organization:
- Indiana Univ., Indianapolis, IN (United States); Kent State Univ., OH (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-98ER14890
- Assignee:
- Indiana University Research and Technology Corporation (Indianapolis, IN); Kent State University (Kent, OH)
- Patent Number(s):
- 11,133,170
- Application Number:
- 16/997,061
- OSTI ID:
- 1840498
- Resource Relation:
- Patent File Date: 08/19/2020
- Country of Publication:
- United States
- Language:
- English
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