Diverse Antibody Responses to Conserved Structural Motifs in Plasmodium falciparum Circumsporozoite Protein

Pholcharee, Tossapol; Oyen, David; Torres, Jonathan L.; Flores-Garcia, Yevel; Martin, Gregory M.; González-Páez, Gonzalo E.; Emerling, Daniel; Volkmuth, Wayne; Locke, Emily; King, C. Richter; Zavala, Fidel; Ward, Andrew B.; Wilson, Ian A.

doi:10.1016/j.jmb.2019.12.029

Diverse Antibody Responses to Conserved Structural Motifs in Plasmodium falciparum Circumsporozoite Protein

Journal Article · Fri Dec 27 00:00:00 EST 2019 · Journal of Molecular Biology

DOI:https://doi.org/10.1016/j.jmb.2019.12.029· OSTI ID:1839762

^[1]; ^[1]; Torres, Jonathan L. ^[1]; Flores-Garcia, Yevel ^[2]; Martin, Gregory M. ^[1]; ^[1]; Emerling, Daniel ^[3]; Volkmuth, Wayne ^[3]; Locke, Emily ^[4]; King, C. Richter ^[4]; Zavala, Fidel ^[2]; ^[1]; Wilson, Ian A. ^[5]

The Scripps Research Inst., La Jolla, CA (United States)
Johns Hopkins Univ., Baltimore, MD (United States). Malaria Research Inst.
Atreca Inc., Redwood City, CA (United States)
PATH Center for Vaccine Innovation and Access, Washington, DC (United States)
The Scripps Research Inst., La Jolla, CA (United States); The Scripps Research Inst., La Jolla, CA (United States). Skaggs Inst. for Chemical Biology

Malaria vaccine candidate RTS,S/AS01 is based on the central and C-terminal regions of the circumsporozoite protein (CSP) of P. falciparum. mAb397 was isolated from a volunteer in an RTS,S/AS01 clinical trial, and it protects mice from infection by malaria sporozoites. However, mAb397 originates from the less commonly used VH3-15 germline gene compared to the VH3-30/33 antibodies generally elicited by RTS,S to the central NANP repeat region of CSP. The crystal structure of mAb397 with an NPNA4 peptide shows that the central NPNA forms a type I β-turn and is the main recognition motif. In most anti-NANP antibodies studied to date, a germline-encoded Trp is used to engage the Pro in NPNA β-turns, but here the Trp interacts with the first Asn. This “conserved” Trp, however, can arise from different germline genes and be located in the heavy or the light chain. Variation in the terminal ψ angles of the NPNA β-turns results in different dispositions of the subsequent NPNA and, hence, different stoichiometries and modes of antibody binding to rsCSP. Diverse protective antibodies against NANP repeats are therefore not limited to a single germline gene response or mode of binding.

View Accepted Manuscript (DOE)

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: Bill and Melinda Gates Foundation; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1839762

Alternate ID(s):: OSTI ID: 1834868

Journal Information:: Journal of Molecular Biology, Journal Name: Journal of Molecular Biology Journal Issue: 4 Vol. 432; ISSN 0022-2836

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
NANP repeats
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sporozoite
type I β-turn

Diverse Antibody Responses to Conserved Structural Motifs in Plasmodium falciparum Circumsporozoite Protein

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