Structure and Characterization of Crimean-Congo Hemorrhagic Fever Virus GP38

Mishra, Akaash K.; Moyer, Crystal L.; Abelson, Dafna M.; Deer, Daniel J.; El Omari, Kamel; Duman, Ramona; Lobel, Leslie; Lutwama, Julius J.; Dye, John M.; Wagner, Armin; Chandran, Kartik; Cross, Robert W.; Geisbert, Thomas W.; Zeitlin, Larry; Bornholdt, Zachary A.; McLellan, Jason S.

doi:10.1128/jvi.02005-19

Structure and Characterization of Crimean-Congo Hemorrhagic Fever Virus GP38

Journal Article · Tue Mar 31 00:00:00 EDT 2020 · Journal of Virology

DOI:https://doi.org/10.1128/jvi.02005-19· OSTI ID:1839757

Mishra, Akaash K. ^[1]; Moyer, Crystal L. ^[2]; Abelson, Dafna M. ^[3]; Deer, Daniel J. ^[4]; El Omari, Kamel ^[5]; Duman, Ramona ^[5]; Lobel, Leslie ^[6]; Lutwama, Julius J. ^[7]; Dye, John M. ^[8]; Wagner, Armin ^[5]; ^[9]; Cross, Robert W. ^[4]; Geisbert, Thomas W. ^[4]; Zeitlin, Larry ^[3]; Bornholdt, Zachary A. ^[3]; ^[1]

Univ. of Texas, Austin, TX (United States)
Mapp Biopharmaceutical, San Diego, California, USA
Mapp Biopharmaceutical, San Diego, CA (United States)
Univ. of Texas Medical Branch, Galveston, TX (United States)
Science and Technology Facilities Council (STFC), Oxford (United Kingdom). Diamond Light Source, Ltd.
Ben-Gurion Univ. of the Negev, Beer-Sheva (Israel)
Uganda Virus Research Inst., Entebbe (Uganda)
US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA
Albert Einstein College of Medicine, New York, NY (United States)

Crimean-Congo hemorrhagic fever virus (CCHFV) is a priority pathogen that poses a high risk to public health. Due to the high morbidity and mortality rates associated with CCHFV infection, there is an urgent need to develop medical countermeasures for disease prevention and treatment. CCHFV GP38, a secreted glycoprotein of unknown function unique to the Nairoviridae family, was recently shown to be the target of a protective antibody against CCHFV. Here, we present the crystal structure of GP38, which revealed a novel fold with distant homology to another CCHFV glycoprotein that is suggestive of a gene duplication event. We also demonstrate that antibody 13G8 protects STAT1-knockout mice against heterologous CCHFV challenge using a clinical isolate from regions where CCHFV is endemic. Collectively, these data advance our understanding of GP38 structure and antigenicity and should facilitate future studies investigating its function.

View Accepted Manuscript (DOE)

Research Organization:: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1839757

Alternate ID(s):: OSTI ID: 1834809

Journal Information:: Journal of Virology, Journal Name: Journal of Virology Journal Issue: 8 Vol. 94; ISSN 0022-538X

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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